7fe2 - Revision history

OCA at 17:14, 29 November 2023

2023-11-29T17:14:43Z

← Older revision		Revision as of 17:14, 29 November 2023
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	<StructureSection load='7fe2' size='340' side='right'caption='[[7fe2]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='7fe2' size='340' side='right'caption='[[7fe2]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7fe2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FE2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7fe2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_ATCC_10100 Enterococcus faecalis ATCC 10100]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FE2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA-D-MANNOSE~~'>~~MAN~~</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fe2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fe2 OCA], [https://pdbe.org/7fe2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fe2 RCSB], [https://www.ebi.ac.uk/pdbsum/7fe2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fe2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fe2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fe2 OCA], [https://pdbe.org/7fe2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fe2 RCSB], [https://www.ebi.ac.uk/pdbsum/7fe2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fe2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q832K9_ENTFA Q832K9_ENTFA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7fe2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7fe2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Mannosidase 3D structures\|Mannosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Enterococcus faecalis ATCC 10100]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Alonso-Gil, S]]		[[Category: Alonso-Gil S]]
	[[Category: Miyazaki, T]]		[[Category: Miyazaki T]]
	~~[[Category: Carbohydrate]]~~
	~~[[Category: Gh92]]~~
	~~[[Category: Glycoside hydrolase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Inhibitor]]~~
	~~[[Category: N-glycan~~]]

OCA at 11:00, 16 February 2022

2022-02-16T11:00:56Z

← Older revision		Revision as of 11:00, 16 February 2022
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	==Crystal structure of the mutant E494Q of GH92 alpha-1,2-mannosidase from Enterococcus faecalis ATCC 10100 in complex with alpha-1,2-mannobiose==		==Crystal structure of the mutant E494Q of GH92 alpha-1,2-mannosidase from Enterococcus faecalis ATCC 10100 in complex with alpha-1,2-mannobiose==
	<StructureSection load='7fe2' size='340' side='right'caption='[[7fe2]]' scene=''>		<StructureSection load='7fe2' size='340' side='right'caption='[[7fe2]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FE2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7fe2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FE2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fe2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fe2 OCA], [https://pdbe.org/7fe2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fe2 RCSB], [https://www.ebi.ac.uk/pdbsum/7fe2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fe2 ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fe2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fe2 OCA], [https://pdbe.org/7fe2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fe2 RCSB], [https://www.ebi.ac.uk/pdbsum/7fe2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fe2 ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The conformational changes in a sugar moiety along the hydrolytic pathway are key to understand the mechanism of glycoside hydrolases (GHs) and to design new inhibitors. The two predominant itineraries for mannosidases go via OS2 --> B2,5 --> 1S5 and 3S1 --> 3H4 --> 1C4. For the CAZy family 92, the conformational itinerary was unknown. Published complexes of Bacteroides thetaiotaomicron GH92 catalyst with a S-glycoside and mannoimidazole indicate a 4C1 --> 4H5/1S5 --> 1S5 mechanism. However, as observed with the GH125 family, S-glycosides may not act always as good mimics of GH's natural substrate. Here we present a cooperative study between computations and experiments where our results predict the E5 --> B2,5/1S5 --> 1S5 pathway for GH92 enzymes. Furthermore, we demonstrate the Michaelis complex mimicry of a new kind of C-disaccharides, whose biochemical applicability was still a chimera.

			Unlocking the hydrolytic mechanism of GH92 alpha-1,2-mannosidases: computation inspires using C-glycosides as Michaelis complex mimics.,Alonso-Gil S, Parkan K, Kaminsky J, Pohl R, Miyazaki T Chemistry. 2022 Jan 20. doi: 10.1002/chem.202200148. PMID:35049087<ref>PMID:35049087</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7fe2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Alonso-Gil S]]		[[Category: Alonso-Gil, S]]
	[[Category: Miyazaki T]]		[[Category: Miyazaki, T]]
			[[Category: Carbohydrate]]
			[[Category: Gh92]]
			[[Category: Glycoside hydrolase]]
			[[Category: Hydrolase]]
			[[Category: Inhibitor]]
			[[Category: N-glycan]]

OCA at 06:59, 2 February 2022

2022-02-02T06:59:04Z

← Older revision		Revision as of 06:59, 2 February 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7fe2 is ~~ON HOLD until Paper Publication~~		==Crystal structure of the mutant E494Q of GH92 alpha-1,2-mannosidase from Enterococcus faecalis ATCC 10100 in complex with alpha-1,2-mannobiose==
			<StructureSection load='7fe2' size='340' side='right'caption='[[7fe2]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FE2 FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fe2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fe2 OCA], [https://pdbe.org/7fe2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fe2 RCSB], [https://www.ebi.ac.uk/pdbsum/7fe2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fe2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Alonso-Gil S]]
			[[Category: Miyazaki T]]

OCA at 07:34, 29 September 2021

2021-09-29T07:34:35Z

← Older revision		Revision as of 07:34, 29 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7fe2 is ON HOLD		The entry 7fe2 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7fe2" [edit=sysop:move=sysop]

2021-07-28T09:20:21Z

Protected "7fe2" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:20, 28 July 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7fe2 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-07-28T09:20:20Z

New page: '''Unreleased structure''' The entry 7fe2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7fe2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]