OCA at 11:21, 8 November 2023

2023-11-08T11:21:00Z

← Older revision		Revision as of 11:21, 8 November 2023
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7ge3 ~~is ON HOLD until Paper Publication~~		==Group deposition SARS-CoV-2 main protease in complex with inhibitors from the COVID Moonshot -- Crystal Structure of SARS-CoV-2 main protease in complex with TRY-UNI-714a760b-3 (Mpro-x11317)==
			<StructureSection load='7ge3' size='340' side='right'caption='[[7ge3]], [[Resolution\|resolution]] 1.27Å' scene=''>
	~~Authors~~: ~~Fearon, D~~.~~, Aimon, A~~.~~, Aschenbrenner, J~~.C.~~, Balcomb, B~~.H.~~, Bertram, F~~.K.R.~~, Brandao~~-~~Neto~~, J., ~~Dias, A~~.~~, Douangamath, A~~., ~~Dunnett, L~~.~~, Godoy, A~~.S., ~~Gorrie-Stone~~, T.J., ~~Koekemoer, L~~.~~, Krojer, T~~., ~~Lithgo, R~~.M.~~, Lukacik, P~~.~~, Marples, P~~.G.~~, Mikolajek, H~~.~~, Nelson, E~~., ~~Owen, C~~.D., ~~Powell, A~~.J., ~~Rangel, V~~.L., ~~Skyner, R~~.~~, Strain~~-~~Damerell~~, C.M.~~, Thompson, W~~.~~, Tomlinson,~~ C.W.E., ~~Wild, C~~., ~~Walsh, M~~.A., ~~von Delft, F~~.		== Structural highlights ==
			<table><tr><td colspan='2'>[[7ge3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7GE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7GE3 FirstGlance]. <br>
	~~Description~~: ~~Group deposition SARS~~-~~CoV~~-2 ~~main protease~~ in ~~complex~~ with ~~inhibitors from~~ the ~~COVID Moonshot~~ --~~Crystal Structure of SARS~~-~~CoV~~-2 ~~main protease in complex with TRY~~-~~UNI~~-~~714a760b~~-~~3 (Mpro~~-~~x11317)~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.27Å</td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=N5L:N-(5-amino-4-methylpyridin-3-yl)-2-(3-chlorophenyl)acetamide'>N5L</scene></td></tr>
	[[Category: Aimon, A]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ge3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ge3 OCA], [https://pdbe.org/7ge3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ge3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ge3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ge3 ProSAT]</span></td></tr>
	[[Category: ~~Rangel, V.L~~]]		</table>
	[[Category: ~~Lukacik, P~~]]		== Function ==
	[[Category: ~~Balcomb, B.H~~]]		[https://www.uniprot.org/uniprot/R1AB_SARS2 R1AB_SARS2] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.[UniProtKB:P0C6X7] Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.[UniProtKB:P0C6X7] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.[UniProtKB:P0C6X7] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN] (PubMed:32198291). Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7]<ref>PMID:32198291</ref> Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7] Responsible for replication and transcription of the viral RNA genome.[UniProtKB:P0C6X7] Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.[UniProtKB:P0C6X7] Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. Acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens.[UniProtKB:P0C6X7] Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.[UniProtKB:P0C6X7] Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.[UniProtKB:P0C6X7]
	[[Category: ~~Strain~~-~~Damerell, C.M~~]]		== References ==
	[[Category: ~~Lithgo, R.M~~]]		<references/>
	[[Category: ~~Dunnett, L~~]]		__TOC__
	[[Category: ~~Brandao-Neto, J~~]]		</StructureSection>
	[[Category: ~~Godoy, A.S~~]]		[[Category: Large Structures]]
	[[Category: ~~Fearon, D~~]]		[[Category: Severe acute respiratory syndrome coronavirus 2]]
	[[Category: ~~Walsh, M.A~~]]		[[Category: Aimon A]]
	[[Category: ~~Nelson, E~~]]		[[Category: Aschenbrenner JC]]
	[[Category: ~~Thompson, W~~]]		[[Category: Balcomb BH]]
	[[Category: ~~Von Delft, F~~]]		[[Category: Bertram FKR]]
	[[Category: ~~Marples,~~ P.G]]		[[Category: Brandao-Neto J]]
	[[Category: ~~Koekemoer, L~~]]		[[Category: Dias A]]
	[[Category: ~~Wild, C~~]]		[[Category: Douangamath A]]
	[[Category: ~~Powell, A.J~~]]		[[Category: Dunnett L]]
	[[Category: ~~Skyner, R~~]]		[[Category: Fearon D]]
	[[Category: ~~Aschenbrenner, J.C~~]]		[[Category: Godoy AS]]
	[[Category: ~~Douangamath, A~~]]		[[Category: Gorrie-Stone TJ]]
	[[Category: ~~Bertram, F.K.~~R]]		[[Category: Koekemoer L]]
	[[Category: ~~Owen, C.D~~]]		[[Category: Krojer T]]
	[[Category: ~~Krojer, T~~]]		[[Category: Lithgo RM]]
	[[Category: Tomlinson~~, C.W.E~~]]		[[Category: Lukacik P]]
	[[Category: ~~Mikolajek, H~~]]		[[Category: Marples PG]]
	[[Category: ~~Gorrie-Stone, T.J~~]]		[[Category: Mikolajek H]]
	[[Category: ~~Dias, A~~]]		[[Category: Nelson E]]
			[[Category: Owen CD]]
			[[Category: Powell AJ]]
			[[Category: Rangel VL]]
			[[Category: Skyner R]]
			[[Category: Strain-Damerell CM]]
			[[Category: Thompson W]]
			[[Category: Tomlinson CWE]]
			[[Category: Walsh MA]]
			[[Category: Wild C]]
			[[Category: Von Delft F]]

OCA at 11:40, 18 October 2023

2023-10-18T11:40:55Z

← Older revision		Revision as of 11:40, 18 October 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7ge3 is ON HOLD		The entry 7ge3 is ON HOLD until Paper Publication

	Authors: Fearon, D., Aimon, A., Aschenbrenner, J.C., Balcomb, B.H., Bertram, F.K.R., Brandao-Neto, J., Dias, A., Douangamath, A., Dunnett, L., Godoy, A.S., Gorrie-Stone, T.J., Koekemoer, L., Krojer, T., Lithgo, R.M., Lukacik, P., Marples, P.G., Mikolajek, H., Nelson, E., Owen, C.D., Powell, A.J., Rangel, V.L., Skyner, R., Strain-Damerell, C.M., Thompson, W., Tomlinson, C.W.E., Wild, C., Walsh, M.A., von Delft, F.		Authors: Fearon, D., Aimon, A., Aschenbrenner, J.C., Balcomb, B.H., Bertram, F.K.R., Brandao-Neto, J., Dias, A., Douangamath, A., Dunnett, L., Godoy, A.S., Gorrie-Stone, T.J., Koekemoer, L., Krojer, T., Lithgo, R.M., Lukacik, P., Marples, P.G., Mikolajek, H., Nelson, E., Owen, C.D., Powell, A.J., Rangel, V.L., Skyner, R., Strain-Damerell, C.M., Thompson, W., Tomlinson, C.W.E., Wild, C., Walsh, M.A., von Delft, F.

OCA: Protected "7ge3" [edit=sysop:move=sysop]

2023-08-23T04:16:34Z

Protected "7ge3" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:16, 23 August 2023
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ge3 is ON HOLD Authors: Fearon, D., Aimon, A., Aschenbrenner, J.C., Balcomb, B.H., Bertram, F.K.R., Brandao-Neto, J., Dias, A., Douangamath, A., Du...

2023-08-23T04:16:33Z

New page: '''Unreleased structure''' The entry 7ge3 is ON HOLD Authors: Fearon, D., Aimon, A., Aschenbrenner, J.C., Balcomb, B.H., Bertram, F.K.R., Brandao-Neto, J., Dias, A., Douangamath, A., Du...

New page

'''Unreleased structure'''

The entry 7ge3 is ON HOLD

Authors: Fearon, D., Aimon, A., Aschenbrenner, J.C., Balcomb, B.H., Bertram, F.K.R., Brandao-Neto, J., Dias, A., Douangamath, A., Dunnett, L., Godoy, A.S., Gorrie-Stone, T.J., Koekemoer, L., Krojer, T., Lithgo, R.M., Lukacik, P., Marples, P.G., Mikolajek, H., Nelson, E., Owen, C.D., Powell, A.J., Rangel, V.L., Skyner, R., Strain-Damerell, C.M., Thompson, W., Tomlinson, C.W.E., Wild, C., Walsh, M.A., von Delft, F.

Description: Group deposition SARS-CoV-2 main protease in complex with inhibitors from the COVID Moonshot --Crystal Structure of SARS-CoV-2 main protease in complex with TRY-UNI-714a760b-3 (Mpro-x11317)
[[Category: Unreleased Structures]]
[[Category: Aimon, A]]
[[Category: Rangel, V.L]]
[[Category: Lukacik, P]]
[[Category: Balcomb, B.H]]
[[Category: Strain-Damerell, C.M]]
[[Category: Lithgo, R.M]]
[[Category: Dunnett, L]]
[[Category: Brandao-Neto, J]]
[[Category: Godoy, A.S]]
[[Category: Fearon, D]]
[[Category: Walsh, M.A]]
[[Category: Nelson, E]]
[[Category: Thompson, W]]
[[Category: Von Delft, F]]
[[Category: Marples, P.G]]
[[Category: Koekemoer, L]]
[[Category: Wild, C]]
[[Category: Powell, A.J]]
[[Category: Skyner, R]]
[[Category: Aschenbrenner, J.C]]
[[Category: Douangamath, A]]
[[Category: Bertram, F.K.R]]
[[Category: Owen, C.D]]
[[Category: Krojer, T]]
[[Category: Tomlinson, C.W.E]]
[[Category: Mikolajek, H]]
[[Category: Gorrie-Stone, T.J]]
[[Category: Dias, A]]

7ge3 - Revision history

OCA at 11:21, 8 November 2023

OCA at 11:40, 18 October 2023

OCA: Protected "7ge3" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ge3 is ON HOLD Authors: Fearon, D., Aimon, A., Aschenbrenner, J.C., Balcomb, B.H., Bertram, F.K.R., Brandao-Neto, J., Dias, A., Douangamath, A., Du...