7jsd - Revision history

OCA at 15:10, 18 October 2023

2023-10-18T15:10:48Z

← Older revision		Revision as of 15:10, 18 October 2023
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	<StructureSection load='7jsd' size='340' side='right'caption='[[7jsd]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='7jsd' size='340' side='right'caption='[[7jsd]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7jsd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"streptomyces_roseofaciens"_van_der_aart_et_al._2018 "streptomyces roseofaciens" van der aart et al. 2018~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JSD FirstGlance]. <br>		<table><tr><td colspan='2'>[[7jsd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_roseifaciens Streptomyces roseifaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JSD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsd OCA], [https://pdbe.org/7jsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsd RCSB], [https://www.ebi.ac.uk/pdbsum/7jsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsd OCA], [https://pdbe.org/7jsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsd RCSB], [https://www.ebi.ac.uk/pdbsum/7jsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsd ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Streptomyces roseofaciens van der aart et al. 2018]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Chang, M C.Y~~]]		[[Category: Streptomyces roseifaciens]]
	[[Category: ~~Kissman, E N~~]]		[[Category: Chang MCY]]
	[[Category: ~~Neugebauer, M E~~]]		[[Category: Kissman EN]]
	[[Category: ~~Biosynthetic protein]]~~		[[Category: Neugebauer ME]]
	~~[[Category: Hydroxylase]]~~
	~~[[Category: Non-heme iron~~]]

OCA at 07:18, 9 March 2022

2022-03-09T07:18:49Z

← Older revision		Revision as of 07:18, 9 March 2022
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	==Hydroxylase homolog of BesD with Fe(II), alpha-ketoglutarate, and lysine==		==Hydroxylase homolog of BesD with Fe(II), alpha-ketoglutarate, and lysine==
	<StructureSection load='7jsd' size='340' side='right'caption='[[7jsd]]' scene=''>		<StructureSection load='7jsd' size='340' side='right'caption='[[7jsd]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JSD FirstGlance]. <br>		<table><tr><td colspan='2'>[[7jsd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_roseofaciens"_van_der_aart_et_al._2018 "streptomyces roseofaciens" van der aart et al. 2018]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JSD FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsd OCA], [https://pdbe.org/7jsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsd RCSB], [https://www.ebi.ac.uk/pdbsum/7jsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsd ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsd OCA], [https://pdbe.org/7jsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsd RCSB], [https://www.ebi.ac.uk/pdbsum/7jsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsd ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Fe(II)/alpha-ketoglutarate (Fe(II)/alphaKG)-dependent enzymes offer a promising biocatalytic platform for halogenation chemistry owing to their ability to functionalize unactivated C-H bonds. However, relatively few radical halogenases have been identified to date, limiting their synthetic utility. Here, we report a strategy to expand the palette of enzymatic halogenation by engineering a reaction pathway rather than substrate selectivity. This approach could allow us to tap the broader class of Fe(II)/alphaKG-dependent hydroxylases as catalysts by their conversion to halogenases. Toward this goal, we discovered active halogenases from a DNA shuffle library generated from a halogenase-hydroxylase pair using a high-throughput in vivo fluorescent screen coupled to an alkyne-producing biosynthetic pathway. Insights from sequencing halogenation-active variants along with the crystal structure of the hydroxylase enabled engineering of a hydroxylase to perform halogenation with comparable activity and higher selectivity than the wild-type halogenase, showcasing the potential of harnessing hydroxylases for biocatalytic halogenation.

			Reaction pathway engineering converts a radical hydroxylase into a halogenase.,Neugebauer ME, Kissman EN, Marchand JA, Pelton JG, Sambold NA, Millar DC, Chang MCY Nat Chem Biol. 2022 Feb;18(2):171-179. doi: 10.1038/s41589-021-00944-x. Epub 2021, Dec 22. PMID:34937913<ref>PMID:34937913</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7jsd" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Streptomyces roseofaciens van der aart et al. 2018]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chang ~~MCY~~]]		[[Category: Chang, M C.Y]]
	[[Category: Kissman EN]]		[[Category: Kissman, E N]]
	[[Category: Neugebauer ME]]		[[Category: Neugebauer, M E]]
			[[Category: Biosynthetic protein]]
			[[Category: Hydroxylase]]
			[[Category: Non-heme iron]]

OCA at 06:19, 25 August 2021

2021-08-25T06:19:01Z

← Older revision		Revision as of 06:19, 25 August 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7jsd is ~~ON HOLD until Aug 14 2022~~		==Hydroxylase homolog of BesD with Fe(II), alpha-ketoglutarate, and lysine==
			<StructureSection load='7jsd' size='340' side='right'caption='[[7jsd]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JSD FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsd OCA], [https://pdbe.org/7jsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsd RCSB], [https://www.ebi.ac.uk/pdbsum/7jsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsd ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Chang MCY]]
			[[Category: Kissman EN]]
			[[Category: Neugebauer ME]]

OCA at 05:00, 11 August 2021

2021-08-11T05:00:13Z

← Older revision		Revision as of 05:00, 11 August 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7jsd is ON HOLD		The entry 7jsd is ON HOLD until Aug 14 2022

	Authors:		Authors:

OCA at 13:34, 17 June 2021

2021-06-17T13:34:53Z

← Older revision		Revision as of 13:34, 17 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7jsd is ON HOLD ~~until Aug 14 2022~~		The entry 7jsd is ON HOLD

	Authors:		Authors:

OCA at 09:03, 23 September 2020

2020-09-23T09:03:28Z

← Older revision		Revision as of 09:03, 23 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7jsd is ON HOLD		The entry 7jsd is ON HOLD until Aug 14 2022

	Authors:		Authors:

OCA: Protected "7jsd" [edit=sysop:move=sysop]

2020-08-26T09:34:50Z

Protected "7jsd" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:34, 26 August 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7jsd is ON HOLD Authors: Description: Category: Unreleased Structures

2020-08-26T09:34:49Z

New page: '''Unreleased structure''' The entry 7jsd is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7jsd is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]