7loo - Revision history

OCA at 15:54, 18 October 2023

2023-10-18T15:54:52Z

← Older revision		Revision as of 15:54, 18 October 2023
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	<StructureSection load='7loo' size='340' side='right'caption='[[7loo]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='7loo' size='340' side='right'caption='[[7loo]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7loo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOO FirstGlance]. <br>		<table><tr><td colspan='2'>[[7loo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_908573 Escherichia coli 908573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7loo OCA], [https://pdbe.org/7loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7loo RCSB], [https://www.ebi.ac.uk/pdbsum/7loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7loo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7loo OCA], [https://pdbe.org/7loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7loo RCSB], [https://www.ebi.ac.uk/pdbsum/7loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7loo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX]] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]		[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7loo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7loo" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[S-adenosylmethionine synthetase 3D structures\|S-adenosylmethionine synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Escherichia coli 908573]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Methionine adenosyltransferase]]~~		[[Category: Jackson CJ]]
	[[Category: Jackson~~, C J~~]]		[[Category: Laurino P]]
	[[Category: Laurino, P]]		[[Category: Tan LL]]
	[[Category: Tan~~, L L]]~~
	~~[[Category: S-adenosyl methionine transferase]]~~
	~~[[Category: Transferase~~]]

OCA at 07:28, 2 March 2022

2022-03-02T07:28:09Z

← Older revision		Revision as of 07:28, 2 March 2022
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	==S-adenosyl methionine transferase cocrystallized with ATP==		==S-adenosyl methionine transferase cocrystallized with ATP==
	<StructureSection load='7loo' size='340' side='right'caption='[[7loo]]' scene=''>		<StructureSection load='7loo' size='340' side='right'caption='[[7loo]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOO FirstGlance]. <br>		<table><tr><td colspan='2'>[[7loo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7loo OCA], [https://pdbe.org/7loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7loo RCSB], [https://www.ebi.ac.uk/pdbsum/7loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7loo ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7loo OCA], [https://pdbe.org/7loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7loo RCSB], [https://www.ebi.ac.uk/pdbsum/7loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7loo ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX]] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Protein conformational changes can facilitate the binding of noncognate substrates and underlying promiscuous activities. However, the contribution of substrate conformational dynamics to this process is comparatively poorly understood. Here, we analyze human (hMAT2A) and Escherichia coli (eMAT) methionine adenosyltransferases that have identical active sites but different substrate specificity. In the promiscuous hMAT2A, noncognate substrates bind in a stable conformation to allow catalysis. In contrast, noncognate substrates sample stable productive binding modes less frequently in eMAT owing to altered mobility in the enzyme active site. Different cellular concentrations of substrates likely drove the evolutionary divergence of substrate specificity in these orthologues. The observation of catalytic promiscuity in hMAT2A led to the detection of a new human metabolite, methyl thioguanosine, that is produced at elevated levels in a cancer cell line. This work establishes that identical active sites can result in different substrate specificity owing to the effects of substrate and enzyme dynamics.

			Substrate Dynamics Contribute to Enzymatic Specificity in Human and Bacterial Methionine Adenosyltransferases.,Gade M, Tan LL, Damry AM, Sandhu M, Brock JS, Delaney A, Villar-Briones A, Jackson CJ, Laurino P JACS Au. 2021 Nov 19;1(12):2349-2360. doi: 10.1021/jacsau.1c00464. eCollection, 2021 Dec 27. PMID:34977903<ref>PMID:34977903</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7loo" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jackson CJ]]		[[Category: Methionine adenosyltransferase]]
	[[Category: Laurino P]]		[[Category: Jackson, C J]]
	[[Category: Tan LL]]		[[Category: Laurino, P]]
			[[Category: Tan, L L]]
			[[Category: S-adenosyl methionine transferase]]
			[[Category: Transferase]]

OCA at 09:26, 15 September 2021

2021-09-15T09:26:40Z

← Older revision		Revision as of 09:26, 15 September 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7loo is ~~ON HOLD until Feb 10 2023~~		==S-adenosyl methionine transferase cocrystallized with ATP==
			<StructureSection load='7loo' size='340' side='right'caption='[[7loo]]' scene=''>
	~~Authors~~: ~~Jackson~~, C.J., ~~Tan~~, L.L., ~~Laurino~~, P.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LOO FirstGlance]. <br>
	~~Description: S-adenosyl methionine transferase cocrystallized with ATP~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7loo OCA], [https://pdbe.org/7loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7loo RCSB], [https://www.ebi.ac.uk/pdbsum/7loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7loo ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Jackson~~, C.J~~]]		__TOC__
	[[Category: Laurino, P]]		</StructureSection>
	[[Category: Tan~~, L.L~~]]		[[Category: Large Structures]]
			[[Category: Jackson CJ]]
			[[Category: Laurino P]]
			[[Category: Tan LL]]

OCA at 05:22, 17 March 2021

2021-03-17T05:22:37Z

← Older revision		Revision as of 05:22, 17 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7loo is ON HOLD		The entry 7loo is ON HOLD until Feb 10 2023

	Authors: Jackson, C.J., Tan, L.L., Laurino, P.		Authors: Jackson, C.J., Tan, L.L., Laurino, P.

OCA: Protected "7loo" [edit=sysop:move=sysop]

2021-02-17T08:49:20Z

Protected "7loo" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:49, 17 February 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7loo is ON HOLD Authors: Jackson, C.J., Tan, L.L., Laurino, P. Description: S-adenosyl methionine transferase cocrystallized with ATP [[Category: U...

2021-02-17T08:49:19Z

New page: '''Unreleased structure''' The entry 7loo is ON HOLD Authors: Jackson, C.J., Tan, L.L., Laurino, P. Description: S-adenosyl methionine transferase cocrystallized with ATP [[Category: U...

New page

'''Unreleased structure'''

The entry 7loo is ON HOLD

Authors: Jackson, C.J., Tan, L.L., Laurino, P.

Description: S-adenosyl methionine transferase cocrystallized with ATP
[[Category: Unreleased Structures]]
[[Category: Jackson, C.J]]
[[Category: Laurino, P]]
[[Category: Tan, L.L]]