OCA at 19:40, 29 May 2024

2024-05-29T19:40:57Z

← Older revision		Revision as of 19:40, 29 May 2024
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	====		==Mtb 70S initiation complex==
	<StructureSection load='7mt2' size='340' side='right'caption='[[7mt2]]' scene=''>		<StructureSection load='7mt2' size='340' side='right'caption='[[7mt2]], [[Resolution\|resolution]] 2.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7mt2]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MT2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mt2 OCA], [https://pdbe.org/7mt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mt2 RCSB], [https://www.ebi.ac.uk/pdbsum/7mt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mt2 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.76Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=4OC:4N,O2-METHYLCYTIDINE-5-MONOPHOSPHATE'>4OC</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mt2 OCA], [https://pdbe.org/7mt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mt2 RCSB], [https://www.ebi.ac.uk/pdbsum/7mt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mt2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/RL32_MYCTU RL32_MYCTU]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			EttA, energy-dependent translational throttle A, is a ribosomal factor that gates ribosome entry into the translation elongation cycle. A detailed understanding of its mechanism of action is limited due to the lack of high-resolution structures along its ATPase cycle. Here we present the cryo-electron microscopy (cryo-EM) structures of EttA from Mycobacterium tuberculosis (Mtb), referred to as MtbEttA, in complex with the Mtb 70S ribosome initiation complex (70SIC) at the pre-hydrolysis (ADPNP) and transition (ADP-VO4) states, and the crystal structure of MtbEttA alone in the post-hydrolysis (ADP) state. We observe that MtbEttA binds the E-site of the Mtb 70SIC, remodeling the P-site tRNA and the ribosomal intersubunit bridge B7a during the ribosomal ratcheting. In return, the rotation of the 30S causes conformational changes in MtbEttA, forcing the two nucleotide-binding sites (NBSs) to alternate to engage each ADPNP in the pre-hydrolysis states, followed by complete engagements of both ADP-VO4 molecules in the ATP-hydrolysis transition states. In the post-hydrolysis state, the conserved ATP-hydrolysis motifs of MtbEttA dissociate from both ADP molecules, leaving two nucleotide-binding domains (NBDs) in an open conformation. These structures reveal a dynamic interplay between MtbEttA and the Mtb ribosome, providing insights into the mechanism of translational regulation by EttA-like proteins.

			Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis.,Cui Z, Li X, Shin J, Gamper H, Hou YM, Sacchettini JC, Zhang J Nat Commun. 2022 Jan 21;13(1):432. doi: 10.1038/s41467-022-28078-1. PMID:35064151<ref>PMID:35064151</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7mt2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Ribosome 3D structures\|Ribosome 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Z~~-disk~~]]		[[Category: Mycobacterium tuberculosis H37Rv]]
			[[Category: Cui Z]]
			[[Category: Zhang J]]

OCA at 07:00, 2 February 2022

2022-02-02T07:00:07Z

← Older revision		Revision as of 07:00, 2 February 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7mt2 is ~~ON HOLD~~		====
			<StructureSection load='7mt2' size='340' side='right'caption='[[7mt2]]' scene=''>
	~~Authors~~: ~~Cui~~, Z., ~~Zhang~~, J.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description: Mtb 70S initiation complex~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mt2 OCA], [https://pdbe.org/7mt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mt2 RCSB], [https://www.ebi.ac.uk/pdbsum/7mt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mt2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Cui,~~ Z]]		__TOC__
	~~[[Category: Zhang, J~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA: Protected "7mt2" [edit=sysop:move=sysop]

2021-05-19T08:58:50Z

Protected "7mt2" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:58, 19 May 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7mt2 is ON HOLD Authors: Cui, Z., Zhang, J. Description: Mtb 70S initiation complex Category: Unreleased Structures Category: Cui, Z [[Cate...

2021-05-19T08:58:48Z

New page: '''Unreleased structure''' The entry 7mt2 is ON HOLD Authors: Cui, Z., Zhang, J. Description: Mtb 70S initiation complex Category: Unreleased Structures Category: Cui, Z [[Cate...

New page

'''Unreleased structure'''

The entry 7mt2 is ON HOLD

Authors: Cui, Z., Zhang, J.

Description: Mtb 70S initiation complex
[[Category: Unreleased Structures]]
[[Category: Cui, Z]]
[[Category: Zhang, J]]

7mt2 - Revision history

OCA at 19:40, 29 May 2024

OCA at 07:00, 2 February 2022

OCA: Protected "7mt2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7mt2 is ON HOLD Authors: Cui, Z., Zhang, J. Description: Mtb 70S initiation complex Category: Unreleased Structures Category: Cui, Z [[Cate...