OCA at 06:35, 21 November 2024

2024-11-21T06:35:11Z

← Older revision		Revision as of 06:35, 21 November 2024
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	====		==Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin==
	<StructureSection load='7mt8' size='340' side='right'caption='[[7mt8]]' scene=''>		<StructureSection load='7mt8' size='340' side='right'caption='[[7mt8]], [[Resolution\|resolution]] 5.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7mt8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MT8 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mt8 OCA], [https://pdbe.org/7mt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7mt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mt8 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 5.8Å, 6 models</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene>, <scene name='pdbligand=SGV:SANGIVAMYCIN'>SGV</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mt8 OCA], [https://pdbe.org/7mt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7mt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mt8 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/GRK1_BOVIN GRK1_BOVIN] Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:12686556, PubMed:16675451, PubMed:21299498). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).[UniProtKB:Q9WVL4]<ref>PMID:12686556</ref> <ref>PMID:16675451</ref> <ref>PMID:21299498</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated GPCRs, thereby priming them for desensitization(1). Although it is unclear how GRKs recognize these receptors(2-4), a conserved region at the GRK N terminus is essential for this process(5-8). Here we report a series of cryo-electron microscopy single-particle reconstructions of light-activated rhodopsin (Rho) bound to rhodopsin kinase (GRK1), wherein the N terminus of GRK1 forms a helix that docks into the open cytoplasmic cleft of Rho. The helix also packs against the GRK1 kinase domain and stabilizes it in an active configuration. The complex is further stabilized by electrostatic interactions between basic residues that are conserved in most GPCRs and acidic residues that are conserved in GRKs. We did not observe any density for the regulator of G-protein signalling homology domain of GRK1 or the C terminus of rhodopsin. Crosslinking with mass spectrometry analysis confirmed these results and revealed dynamic behaviour in receptor-bound GRK1 that would allow the phosphorylation of multiple sites in the receptor tail. We have identified GRK1 residues whose mutation augments kinase activity and crosslinking with Rho*, as well as residues that are involved in activation by acidic phospholipids. From these data, we present a general model for how a small family of protein kinases can recognize and be activated by hundreds of different GPCRs.

			Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.,Chen Q, Plasencia M, Li Z, Mukherjee S, Patra D, Chen CL, Klose T, Yao XQ, Kossiakoff AA, Chang L, Andrews PC, Tesmer JJG Nature. 2021 Jul;595(7868):600-605. doi: 10.1038/s41586-021-03721-x. Epub 2021 , Jul 14. PMID:34262173<ref>PMID:34262173</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7mt8" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Rhodopsin kinase\|Rhodopsin kinase]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Z-~~disk~~]]		[[Category: Chen C-L]]
			[[Category: Chen Q]]
			[[Category: Tesmer JJG]]

OCA at 09:57, 7 July 2021

2021-07-07T09:57:56Z

← Older revision		Revision as of 09:57, 7 July 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7mt8 ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7mt8' size='340' side='right'caption='[[7mt8]]' scene=''>
	~~Authors~~: ~~Chen, Q~~.~~, Chen, C~~.-L.~~, Tesmer, J~~.J.G.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Rhodopsin kinase (GRK1)-S5E~~/~~S488E~~/~~T489E in complex with rhodopsin~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mt8 OCA], [https://pdbe.org/7mt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7mt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mt8 ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[~~Category~~: ~~Tesmer~~, J.J.G]]		__TOC__
	[[Category: ~~Chen, C.-L~~]]		</StructureSection>
	[[Category: ~~Chen, Q~~]]		[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 11:05, 9 June 2021

2021-06-09T11:05:51Z

← Older revision		Revision as of 11:05, 9 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7mt8 is ON HOLD		The entry 7mt8 is ON HOLD until Paper Publication

	Authors: Chen, Q., Chen, C.-L., Tesmer, J.J.G.		Authors: Chen, Q., Chen, C.-L., Tesmer, J.J.G.

OCA: Protected "7mt8" [edit=sysop:move=sysop]

2021-05-19T08:59:19Z

Protected "7mt8" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:59, 19 May 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7mt8 is ON HOLD Authors: Chen, Q., Chen, C.-L., Tesmer, J.J.G. Description: Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin [[Cat...

2021-05-19T08:59:18Z

New page: '''Unreleased structure''' The entry 7mt8 is ON HOLD Authors: Chen, Q., Chen, C.-L., Tesmer, J.J.G. Description: Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin [[Cat...

New page

'''Unreleased structure'''

The entry 7mt8 is ON HOLD

Authors: Chen, Q., Chen, C.-L., Tesmer, J.J.G.

Description: Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin
[[Category: Unreleased Structures]]
[[Category: Tesmer, J.J.G]]
[[Category: Chen, C.-L]]
[[Category: Chen, Q]]

7mt8 - Revision history

OCA at 06:35, 21 November 2024

OCA at 09:57, 7 July 2021

OCA at 11:05, 9 June 2021

OCA: Protected "7mt8" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7mt8 is ON HOLD Authors: Chen, Q., Chen, C.-L., Tesmer, J.J.G. Description: Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin [[Cat...