OCA at 08:58, 14 July 2024

2024-07-14T08:58:36Z

← Older revision		Revision as of 08:58, 14 July 2024
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	====		==Rhinovirus-14 ICAM-1 empty particle at pH 6.2==
	<StructureSection load='7num' size='340' side='right'caption='[[7num]]' scene=''>		<StructureSection load='7num' size='340' side='right'caption='[[7num]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7num]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhinovirus_B14 Rhinovirus B14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NUM FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7num OCA], [https://pdbe.org/7num PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7num RCSB], [https://www.ebi.ac.uk/pdbsum/7num PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7num ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.9Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7num OCA], [https://pdbe.org/7num PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7num RCSB], [https://www.ebi.ac.uk/pdbsum/7num PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7num ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/POLG_HRV14 POLG_HRV14] Capsid proteins VP1, VP2, VP3 and VP4 form a closed capsid enclosing the viral positive strand RNA genome. VP4 lies on the inner surface of the protein shell formed by VP1, VP2 and VP3. All the three latter proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, VP2, and VP3, with a diameter of approximately 300 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes (By similarity). The capsid interacts with human ICAM1 to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis. VP0 precursor is a component of immature procapsids (By similarity). Protein 2A is a cysteine protease that is responsible for the cleavage between the P1 and P2 regions. It cleaves the host translation initiation factor EIF4G1, in order to shut down the capped cellular mRNA transcription (By similarity). Protein 2B affects membrane integrity and cause an increase in membrane permeability (By similarity). Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity). Protein 3A, via its hydrophobic domain, serves as membrane anchor (By similarity). Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity). RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Most rhinoviruses, which are the leading cause of the common cold, utilize intercellular adhesion molecule-1 (ICAM-1) as a receptor to infect cells. To release their genomes, rhinoviruses convert to activated particles that contain pores in the capsid, lack minor capsid protein VP4, and have an altered genome organization. The binding of rhinoviruses to ICAM-1 promotes virus activation; however, the molecular details of the process remain unknown. Here, we present the structures of virion of rhinovirus 14 and its complex with ICAM-1 determined to resolutions of 2.6 and 2.4 A, respectively. The cryo-electron microscopy reconstruction of rhinovirus 14 virions contains the resolved density of octanucleotide segments from the RNA genome that interact with VP2 subunits. We show that the binding of ICAM-1 to rhinovirus 14 is required to prime the virus for activation and genome release at acidic pH. Formation of the rhinovirus 14-ICAM-1 complex induces conformational changes to the rhinovirus 14 capsid, including translocation of the C termini of VP4 subunits, which become poised for release through pores that open in the capsids of activated particles. VP4 subunits with altered conformation block the RNA-VP2 interactions and expose patches of positively charged residues. The conformational changes to the capsid induce the redistribution of the virus genome by altering the capsid-RNA interactions. The restructuring of the rhinovirus 14 capsid and genome prepares the virions for conversion to activated particles. The high-resolution structure of rhinovirus 14 in complex with ICAM-1 explains how the binding of uncoating receptors enables enterovirus genome release.

			ICAM-1 induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating.,Hrebik D, Fuzik T, Gondova M, Smerdova L, Adamopoulos A, Sedo O, Zdrahal Z, Plevka P Proc Natl Acad Sci U S A. 2021 May 11;118(19):e2024251118. doi: , 10.1073/pnas.2024251118. PMID:33947819<ref>PMID:33947819</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7num" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Human rhinovirus\|Human rhinovirus]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Rhinovirus B14]]
			[[Category: Hrebik D]]
			[[Category: Plevka P]]

OCA at 10:11, 19 May 2021

2021-05-19T10:11:34Z

← Older revision		Revision as of 10:11, 19 May 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7num ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7num' size='340' side='right'caption='[[7num]]' scene=''>
	~~Authors~~: ~~Hrebik, D~~.~~, Plevka, P~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Rhinovirus~~-~~14 ICAM-1 empty particle at pH 6~~.2		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7num OCA], [https://pdbe.org/7num PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7num RCSB], [https://www.ebi.ac.uk/pdbsum/7num PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7num ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[Category: ~~Plevka, P~~]]		__TOC__
	[[Category: ~~Hrebik, D~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 05:46, 14 April 2021

2021-04-14T05:46:17Z

← Older revision		Revision as of 05:46, 14 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7num is ON HOLD		The entry 7num is ON HOLD until Paper Publication

	Authors: Hrebik, D., Plevka, P.		Authors: Hrebik, D., Plevka, P.

OCA: Protected "7num" [edit=sysop:move=sysop]

2021-03-24T05:38:28Z

Protected "7num" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:38, 24 March 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7num is ON HOLD Authors: Hrebik, D., Plevka, P. Description: Rhinovirus-14 ICAM-1 empty particle at pH 6.2 Category: Unreleased Structures [[Ca...

2021-03-24T05:38:27Z

New page: '''Unreleased structure''' The entry 7num is ON HOLD Authors: Hrebik, D., Plevka, P. Description: Rhinovirus-14 ICAM-1 empty particle at pH 6.2 Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 7num is ON HOLD

Authors: Hrebik, D., Plevka, P.

Description: Rhinovirus-14 ICAM-1 empty particle at pH 6.2
[[Category: Unreleased Structures]]
[[Category: Plevka, P]]
[[Category: Hrebik, D]]

7num - Revision history

OCA at 08:58, 14 July 2024

OCA at 10:11, 19 May 2021

OCA at 05:46, 14 April 2021

OCA: Protected "7num" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7num is ON HOLD Authors: Hrebik, D., Plevka, P. Description: Rhinovirus-14 ICAM-1 empty particle at pH 6.2 Category: Unreleased Structures [[Ca...