7oby - Revision history

OCA at 13:50, 6 November 2024

2024-11-06T13:50:41Z

← Older revision		Revision as of 13:50, 6 November 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.		[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The natural product family of the fusicoccanes (FCs) has been shown to display anti-cancer activity, especially when combined with established therapeutic agents. FCs stabilize 14-3-3 protein-protein interactions (PPIs). Here, we tested combinations of a small library of FCs with interferon alpha (IFNalpha) on different cancer cell lines and report a proteomics approach to identify the specific 14-3-3 PPIs that are induced by IFNalpha and stabilized by FCs in OVCAR-3 cells. Among the identified 14-3-3 target proteins are THEMIS2, receptor interacting protein kinase 2 (RIPK2), EIF2AK2, and several members of the LDB1 complex. Biophysical and structural biology studies confirm these 14-3-3 PPIs as physical targets of FC stabilization, and transcriptome as well as pathway analyses suggest possible explanations for the observed synergistic effect of IFNalpha/FC treatment on cancer cells. This study elucidates the polypharmacological effects of FCs in cancer cells and identifies potential targets from the vast interactome of 14-3-3s for therapeutic intervention in oncology.

			IFNalpha primes cancer cells for Fusicoccin-induced cell death via 14-3-3 PPI stabilization.,Andlovic B, Heilmann G, Ninck S, Andrei SA, Centorrino F, Higuchi Y, Kato N, Brunsveld L, Arkin M, Menninger S, Choidas A, Wolf A, Klebl B, Kaschani F, Kaiser M, Eickhoff J, Ottmann C Cell Chem Biol. 2023 Jun 15;30(6):573-590.e6. doi: , 10.1016/j.chembiol.2023.04.005. Epub 2023 May 1. PMID:37130519<ref>PMID:37130519</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7oby" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]		*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:52, 7 February 2024

2024-02-07T07:52:59Z

← Older revision		Revision as of 07:52, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7oby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OBY FirstGlance]. <br>		<table><tr><td colspan='2'>[[7oby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OBY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FSC:FUSICOCCIN'>FSC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FSC:FUSICOCCIN'>FSC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oby OCA], [https://pdbe.org/7oby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oby RCSB], [https://www.ebi.ac.uk/pdbsum/7oby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oby ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oby OCA], [https://pdbe.org/7oby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oby RCSB], [https://www.ebi.ac.uk/pdbsum/7oby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oby ProSAT]</span></td></tr>
	</table>		</table>

OCA at 05:45, 7 June 2023

2023-06-07T05:45:22Z

← Older revision		Revision as of 05:45, 7 June 2023
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	====		==Crystal structure of 14-3-3 sigma in complex with SSBP4 phosphopeptide and stabilizer Fusicoccin-A==
	<StructureSection load='7oby' size='340' side='right'caption='[[7oby]]' scene=''>		<StructureSection load='7oby' size='340' side='right'caption='[[7oby]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7oby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OBY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oby OCA], [https://pdbe.org/7oby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oby RCSB], [https://www.ebi.ac.uk/pdbsum/7oby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oby ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FSC:FUSICOCCIN'>FSC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oby OCA], [https://pdbe.org/7oby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oby RCSB], [https://www.ebi.ac.uk/pdbsum/7oby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oby ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.

			==See Also==
			*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Andlovic B]]
			[[Category: Centorrino F]]
			[[Category: Ottmann C]]

OCA at 12:48, 4 May 2022

2022-05-04T12:48:01Z

← Older revision		Revision as of 12:48, 4 May 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7oby ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7oby' size='340' side='right'caption='[[7oby]]' scene=''>
	~~Authors~~: ~~Centorrino, F~~.~~, Andlovic, B~~.~~, Ottmann, C~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Crystal structure of 14-3-3 sigma in complex with SSBP4 phosphopeptide and stabilizer Fusicoccin-A~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oby OCA], [https://pdbe.org/7oby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oby RCSB], [https://www.ebi.ac.uk/pdbsum/7oby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oby ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[~~Category~~: ~~Andlovic~~, B]]		__TOC__
	[[Category: ~~Ottmann, C~~]]		</StructureSection>
	[[Category: ~~Centorrino, F~~]]		[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 08:05, 26 May 2021

2021-05-26T08:05:33Z

← Older revision		Revision as of 08:05, 26 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7oby is ON HOLD		The entry 7oby is ON HOLD until Paper Publication

	Authors: Centorrino, F., Andlovic, B., Ottmann, C.		Authors: Centorrino, F., Andlovic, B., Ottmann, C.

OCA: Protected "7oby" [edit=sysop:move=sysop]

2021-05-05T07:35:50Z

Protected "7oby" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:35, 5 May 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7oby is ON HOLD Authors: Centorrino, F., Andlovic, B., Ottmann, C. Description: Crystal structure of 14-3-3 sigma in complex with SSBP4 phosphopept...

2021-05-05T07:35:49Z

New page: '''Unreleased structure''' The entry 7oby is ON HOLD Authors: Centorrino, F., Andlovic, B., Ottmann, C. Description: Crystal structure of 14-3-3 sigma in complex with SSBP4 phosphopept...

New page

'''Unreleased structure'''

The entry 7oby is ON HOLD

Authors: Centorrino, F., Andlovic, B., Ottmann, C.

Description: Crystal structure of 14-3-3 sigma in complex with SSBP4 phosphopeptide and stabilizer Fusicoccin-A
[[Category: Unreleased Structures]]
[[Category: Andlovic, B]]
[[Category: Ottmann, C]]
[[Category: Centorrino, F]]