OCA at 09:00, 14 July 2024

2024-07-14T09:00:06Z

← Older revision		Revision as of 09:00, 14 July 2024
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	==Hepatitis B core protein -low secretion phenotype L60V==		==Hepatitis B core protein -low secretion phenotype L60V==
	<StructureSection load='7oco' size='340' side='right'caption='[[7oco]]' scene=''>		<StructureSection load='7oco' size='340' side='right'caption='[[7oco]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCO FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oco OCA], [https://pdbe.org/7oco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oco RCSB], [https://www.ebi.ac.uk/pdbsum/7oco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oco ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.2Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oco OCA], [https://pdbe.org/7oco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oco RCSB], [https://www.ebi.ac.uk/pdbsum/7oco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oco ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Hepatitis B virus is a major human pathogen, which forms enveloped virus particles. During viral maturation, membrane-bound hepatitis B surface proteins package hepatitis B core protein capsids. This process is intercepted by certain peptides with an "LLGRMKG" motif that binds to the capsids at the tips of dimeric spikes. With microcalorimetry, electron cryo microscopy and peptide microarray-based screens, we have characterized the structural and thermodynamic properties of peptide binding to hepatitis B core protein capsids with different secretion phenotypes. The peptide "GSLLGRMKGA" binds weakly to hepatitis B core protein capsids and mutant capsids with a premature (F97L) or low-secretion phenotype (L60V and P5T). With electron cryo microscopy, we provide novel structures for L60V and P5T and demonstrate that binding occurs at the tips of the spikes at the dimer interface, splaying the helices apart independent of the secretion phenotype. Peptide array screening identifies "SLLGRM" as the core binding motif. This shortened motif binds only to one of the two spikes in the asymmetric unit of the capsid and induces a much smaller conformational change. Altogether, these comprehensive studies suggest that the tips of the spikes act as an autonomous binding platform that is unaffected by mutations that affect secretion phenotypes.

			Conformational Plasticity of Hepatitis B Core Protein Spikes Promotes Peptide Binding Independent of the Secretion Phenotype.,Makbul C, Khayenko V, Maric HM, Bottcher B Microorganisms. 2021 Apr 29;9(5). pii: microorganisms9050956. doi:, 10.3390/microorganisms9050956. PMID:33946808<ref>PMID:33946808</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7oco" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:21, 26 May 2021

2021-05-26T09:21:39Z

← Older revision		Revision as of 09:21, 26 May 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7oco is ~~ON HOLD~~		==Hepatitis B core protein -low secretion phenotype L60V==
			<StructureSection load='7oco' size='340' side='right'caption='[[7oco]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCO FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oco OCA], [https://pdbe.org/7oco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oco RCSB], [https://www.ebi.ac.uk/pdbsum/7oco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oco ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Bottcher B]]
			[[Category: Makbul C]]

OCA: Protected "7oco" [edit=sysop:move=sysop]

2021-05-05T07:37:27Z

Protected "7oco" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:37, 5 May 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7oco is ON HOLD Authors: Description: Category: Unreleased Structures

2021-05-05T07:37:26Z

New page: '''Unreleased structure''' The entry 7oco is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7oco is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7oco - Revision history

OCA at 09:00, 14 July 2024

OCA at 09:21, 26 May 2021

OCA: Protected "7oco" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7oco is ON HOLD Authors: Description: Category: Unreleased Structures