7ocr - Revision history

OCA at 12:49, 1 February 2024

2024-02-01T12:49:02Z

← Older revision		Revision as of 12:49, 1 February 2024
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	==NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD from Acinetobacter baumannii==		==NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD from Acinetobacter baumannii==
	<StructureSection load='7ocr' size='340' side='right'caption='[[7ocr]]' scene=''>		<StructureSection load='7ocr' size='340' side='right'caption='[[7ocr]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCR FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ocr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii_ATCC_19606_=_CIP_70.34_=_JCM_6841 Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCR FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ocr OCA], [https://pdbe.org/7ocr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ocr RCSB], [https://www.ebi.ac.uk/pdbsum/7ocr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ocr ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ocr OCA], [https://pdbe.org/7ocr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ocr RCSB], [https://www.ebi.ac.uk/pdbsum/7ocr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ocr ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/D0C7J2_ACIB2 D0C7J2_ACIB2]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			SignificanceMannitol biosynthesis is essential for Acinetobacter baumannii to cope with osmotic stress. Currently, only Pseudomonas putida, Acinetobacter baylyi, and A. baumannii are able to de novo synthesize mannitol by a structurally unique bifunctional mannitol-1-phosphate dehydrogenase/phosphatase (AbMtlD). The molecular mechanism of reduction and dephosphorylation of fructose-6-phosphate to mannitol is highly dependent on the substrate shuffling from one protomer to the other protomer by a unique helix-loop-helix domain-mediated dimer formation, thus ensuring unidirectional and efficient biosynthesis of mannitol. These observations support an evolutionary adaptation of AbMtlD by fusion of dehydrogenase and phosphatase domains to facilitate efficient unidirectional enzymatic production of mannitol, unifying regulatory control and minimizing the intracellular concentration of toxic mannitol-1-phosphate during salt stress.

			Unidirectional mannitol synthesis of Acinetobacter baumannii MtlD is facilitated by the helix-loop-helix-mediated dimer formation.,Tam HK, Konig P, Himpich S, Ngu ND, Abele R, Muller V, Pos KM Proc Natl Acad Sci U S A. 2022 Apr 5;119(14):e2107994119. doi:, 10.1073/pnas.2107994119. Epub 2022 Apr 1. PMID:35363566<ref>PMID:35363566</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ocr" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Acinetobacter baumannii ATCC 19606 = CIP 70 34 = JCM 6841]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mueller V]]		[[Category: Mueller V]]
	[[Category: Pos KM]]		[[Category: Pos KM]]
	[[Category: Tam HK]]		[[Category: Tam HK]]

OCA at 02:54, 21 April 2022

2022-04-21T02:54:29Z

← Older revision		Revision as of 02:54, 21 April 2022
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	~~'''Unreleased structure'''~~

	~~The entry 7ocr is ON HOLD until Paper Publication~~		==NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD from Acinetobacter baumannii==
			<StructureSection load='7ocr' size='340' side='right'caption='[[7ocr]]' scene=''>
	~~Authors: Tam, H.K., Mueller, V., Pos, K.M.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCR FirstGlance]. <br>
	~~Description:~~ NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD from Acinetobacter baumannii		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ocr OCA], [https://pdbe.org/7ocr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ocr RCSB], [https://www.ebi.ac.uk/pdbsum/7ocr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ocr ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Mueller, V]]		__TOC__
	[[Category: ~~Tam, H.K~~]]		</StructureSection>
	[[Category: ~~Pos, K.M~~]]		[[Category: Large Structures]]
			[[Category: Mueller V]]
			[[Category: Pos KM]]
			[[Category: Tam HK]]

OCA at 08:22, 12 May 2021

2021-05-12T08:22:01Z

← Older revision		Revision as of 08:22, 12 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7ocr is ON HOLD		The entry 7ocr is ON HOLD until Paper Publication

	Authors: Tam, H.K., Mueller, V., Pos, K.M.		Authors: Tam, H.K., Mueller, V., Pos, K.M.

OCA: Protected "7ocr" [edit=sysop:move=sysop]

2021-05-05T07:37:35Z

Protected "7ocr" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:37, 5 May 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ocr is ON HOLD Authors: Tam, H.K., Mueller, V., Pos, K.M. Description: NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bif...

2021-05-05T07:37:34Z

New page: '''Unreleased structure''' The entry 7ocr is ON HOLD Authors: Tam, H.K., Mueller, V., Pos, K.M. Description: NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bif...

New page

'''Unreleased structure'''

The entry 7ocr is ON HOLD

Authors: Tam, H.K., Mueller, V., Pos, K.M.

Description: NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD from Acinetobacter baumannii
[[Category: Unreleased Structures]]
[[Category: Mueller, V]]
[[Category: Tam, H.K]]
[[Category: Pos, K.M]]