OCA at 09:01, 14 July 2024

2024-07-14T09:01:05Z

← Older revision		Revision as of 09:01, 14 July 2024
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	====		==nucleosome with TBP and TFIIA bound at SHL +2==
	<StructureSection load='7oha' size='340' side='right'caption='[[7oha]]' scene=''>		<StructureSection load='7oha' size='340' side='right'caption='[[7oha]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7oha]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae], [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oha OCA], [https://pdbe.org/7oha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oha RCSB], [https://www.ebi.ac.uk/pdbsum/7oha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oha ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.9Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oha OCA], [https://pdbe.org/7oha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oha RCSB], [https://www.ebi.ac.uk/pdbsum/7oha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oha ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/H4_XENLA H4_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The TATA box-binding protein (TBP) is highly conserved throughout eukaryotes and plays a central role in the assembly of the transcription preinitiation complex (PIC) at gene promoters. TBP binds and bends DNA, and directs adjacent binding of the transcription factors TFIIA and TFIIB for PIC assembly. Here, we show that yeast TBP can bind to a nucleosome containing the Widom-601 sequence and that TBP-nucleosome binding is stabilized by TFIIA. We determine three cryo-electron microscopy (cryo-EM) structures of TBP-nucleosome complexes, two of them containing also TFIIA. TBP can bind to superhelical location (SHL) -6, which contains a TATA-like sequence, but also to SHL +2, which is GC-rich. Whereas binding to SHL -6 can occur in the absence of TFIIA, binding to SHL +2 is only observed in the presence of TFIIA and goes along with detachment of upstream terminal DNA from the histone octamer. TBP-nucleosome complexes are sterically incompatible with PIC assembly, explaining why a promoter nucleosome generally impairs transcription and must be moved before initiation can occur.

			Structures and implications of TBP-nucleosome complexes.,Wang H, Xiong L, Cramer P Proc Natl Acad Sci U S A. 2021 Jul 27;118(30):e2108859118. doi: , 10.1073/pnas.2108859118. PMID:34301908<ref>PMID:34301908</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7oha" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Histone 3D structures\|Histone 3D structures]]
			*[[TATA-binding protein 3D structures\|TATA-binding protein 3D structures]]
			*[[Transcription initiation factors 3D structures\|Transcription initiation factors 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Saccharomyces cerevisiae]]
			[[Category: Synthetic construct]]
			[[Category: Xenopus laevis]]
			[[Category: Cramer P]]
			[[Category: Wang H]]

OCA at 10:36, 28 July 2021

2021-07-28T10:36:00Z

← Older revision		Revision as of 10:36, 28 July 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7oha is ~~ON HOLD until Paper Publication~~		====
			<StructureSection load='7oha' size='340' side='right'caption='[[7oha]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oha OCA], [https://pdbe.org/7oha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oha RCSB], [https://www.ebi.ac.uk/pdbsum/7oha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oha ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 08:06, 26 May 2021

2021-05-26T08:06:55Z

← Older revision		Revision as of 08:06, 26 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7oha is ON HOLD		The entry 7oha is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7oha" [edit=sysop:move=sysop]

2021-05-19T09:05:57Z

Protected "7oha" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:05, 19 May 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7oha is ON HOLD Authors: Description: Category: Unreleased Structures

2021-05-19T09:05:56Z

New page: '''Unreleased structure''' The entry 7oha is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7oha is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7oha - Revision history

OCA at 09:01, 14 July 2024

OCA at 10:36, 28 July 2021

OCA at 08:06, 26 May 2021

OCA: Protected "7oha" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7oha is ON HOLD Authors: Description: Category: Unreleased Structures