7pp2 - Revision history

OCA at 13:07, 1 February 2024

2024-02-01T13:07:06Z

← Older revision		Revision as of 13:07, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7pp2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] and [https://en.wikipedia.org/wiki/Pyricularia_oryzae Pyricularia oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7pp2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] and [https://en.wikipedia.org/wiki/Pyricularia_oryzae Pyricularia oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PP2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.69Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pp2 OCA], [https://pdbe.org/7pp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pp2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pp2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pp2 OCA], [https://pdbe.org/7pp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pp2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pp2 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:45, 11 January 2023

2023-01-11T07:45:44Z

← Older revision		Revision as of 07:45, 11 January 2023
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	==~~n/a~~==		==Complex of rice blast (Magnaporthe oryzae) effector protein AVR-Pii with the host target Exo70F2 from Rice (Oryza sativa)==
	<StructureSection load='7pp2' size='340' side='right'caption='[[7pp2]]' scene=''>		<StructureSection load='7pp2' size='340' side='right'caption='[[7pp2]], [[Resolution\|resolution]] 2.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7pp2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] and [https://en.wikipedia.org/wiki/Pyricularia_oryzae Pyricularia oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PP2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pp2 OCA], [https://pdbe.org/7pp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pp2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pp2 ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pp2 OCA], [https://pdbe.org/7pp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pp2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pp2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q6K647_ORYSJ Q6K647_ORYSJ] Component of the exocyst complex.[RuleBase:RU365026]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Exocytosis plays an important role in plant-microbe interactions, in both pathogenesis and symbiosis. Exo70 proteins are integral components of the exocyst, an octameric complex that mediates tethering of vesicles to membranes in eukaryotes. Although plant Exo70s are known to be targeted by pathogen effectors, the underpinning molecular mechanisms and the impact of this interaction on infection are poorly understood. Here, we show the molecular basis of the association between the effector AVR-Pii of the blast fungus Maganaporthe oryzae and rice Exo70 alleles OsExo70F2 and OsExo70F3, which is sensed by the immune receptor pair Pii via an integrated RIN4/NOI domain. The crystal structure of AVR-Pii in complex with OsExo70F2 reveals that the effector binds to a conserved hydrophobic pocket in Exo70, defining an effector/target binding interface. Structure-guided and random mutagenesis validates the importance of AVR-Pii residues at the Exo70 binding interface to sustain protein association and disease resistance in rice when challenged with fungal strains expressing effector mutants. Furthermore, the structure of AVR-Pii defines a zinc-finger effector fold (ZiF) distinct from the MAX (Magnaporthe Avrs and ToxB-like) fold previously described for a majority of characterized M. oryzae effectors. Our data suggest that blast fungus ZiF effectors bind a conserved Exo70 interface to manipulate plant exocytosis and that these effectors are also baited by plant immune receptors, pointing to new opportunities for engineering disease resistance.

			A blast fungus zinc-finger fold effector binds to a hydrophobic pocket in host Exo70 proteins to modulate immune recognition in rice.,De la Concepcion JC, Fujisaki K, Bentham AR, Cruz Mireles N, Sanchez de Medina Hernandez V, Shimizu M, Lawson DM, Kamoun S, Terauchi R, Banfield MJ Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2210559119. doi: , 10.1073/pnas.2210559119. Epub 2022 Oct 17. PMID:36252011<ref>PMID:36252011</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7pp2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~N/a~~]]		[[Category: Oryza sativa]]
			[[Category: Pyricularia oryzae]]
			[[Category: Banfield MJ]]
			[[Category: Bentham AR]]
			[[Category: De la Concepcion JC]]
			[[Category: Lawson D]]

OCA at 07:26, 29 June 2022

2022-06-29T07:26:24Z

← Older revision		Revision as of 07:26, 29 June 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7pp2 is ~~ON HOLD until Paper Publication~~		==n/a==
			<StructureSection load='7pp2' size='340' side='right'caption='[[7pp2]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PP2 FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pp2 OCA], [https://pdbe.org/7pp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pp2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pp2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: N/a]]

OCA at 10:13, 13 October 2021

2021-10-13T10:13:25Z

← Older revision		Revision as of 10:13, 13 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7pp2 is ON HOLD		The entry 7pp2 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7pp2" [edit=sysop:move=sysop]

2021-09-22T05:30:44Z

Protected "7pp2" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:30, 22 September 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7pp2 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-09-22T05:30:43Z

New page: '''Unreleased structure''' The entry 7pp2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pp2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]