7ref - Revision history

OCA at 13:58, 6 November 2024

2024-11-06T13:58:04Z

← Older revision		Revision as of 13:58, 6 November 2024
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	<StructureSection load='7ref' size='340' side='right'caption='[[7ref]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='7ref' size='340' side='right'caption='[[7ref]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7ref]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_smegmatis"_trevisan_1889 "bacillus smegmatis" trevisan 1889].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REF FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REF FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ERS451418_03468 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1772~~ "~~Bacillus smegmatis~~" ~~Trevisan 1889])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ref OCA], [https://pdbe.org/7ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ref RCSB], [https://www.ebi.ac.uk/pdbsum/7ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ref ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ref OCA], [https://pdbe.org/7ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ref RCSB], [https://www.ebi.ac.uk/pdbsum/7ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ref ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Iron is essential for growth of Mycobacterium tuberculosis, the causative agent of tuberculosis. To acquire iron from the host, M. tuberculosis uses the siderophores called mycobactins and carboxymycobactins. Here, we show that the rv0455c gene is essential for M. tuberculosis to grow in low-iron medium and that secretion of both mycobactins and carboxymycobactins is drastically reduced in the rv0455c deletion mutant. Both water-soluble and membrane-anchored Rv0455c are functional in siderophore secretion, supporting an intracellular role. Lack of Rv0455c results in siderophore toxicity, a phenotype observed for other siderophore secretion mutants, and severely impairs replication of M. tuberculosis in mice, demonstrating the importance of Rv0455c and siderophore secretion during disease. The crystal structure of a Rv0455c homolog reveals a novel protein fold consisting of a helical bundle with a 'cinch' formed by an essential intramolecular disulfide bond. These findings advance our understanding of the distinct M. tuberculosis siderophore secretion system.

	A periplasmic cinched protein is required for siderophore secretion and virulence of Mycobacterium tuberculosis.,Zhang L, Kent JE, Whitaker M, Young DC, Herrmann D, Aleshin AE, Ko YH, Cingolani G, Saad JS, Moody DB, Marassi FM, Ehrt S, Niederweis M Nat Commun. 2022 Apr 26;13(1):2255. doi: 10.1038/s41467-022-29873-6. PMID:35474308<ref>PMID:35474308</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7ref" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Bacillus smegmatis trevisan 1889]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Aleshin~~, A E~~]]		[[Category: Aleshin AE]]
	[[Category: Kent~~, J E~~]]		[[Category: Kent JE]]
	[[Category: Marassi~~, F M~~]]		[[Category: Marassi FM]]
	[[Category: Niederweis, M]]		[[Category: Niederweis M]]
	[[Category: Zhang, L]]		[[Category: Zhang L]]
	~~[[Category: Mycobacterium smegmatis siderophore secretion]]~~
	~~[[Category: Unknown function~~]]

OCA at 05:36, 8 June 2022

2022-06-08T05:36:09Z

← Older revision		Revision as of 05:36, 8 June 2022
Line 1:		Line 1:

	==Structure of MS3494 from Mycobacterium smegmatis==		==Structure of MS3494 from Mycobacterium smegmatis==
	<StructureSection load='7ref' size='340' side='right'caption='[[7ref]]' scene=''>		<StructureSection load='7ref' size='340' side='right'caption='[[7ref]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REF FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ref]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_smegmatis"_trevisan_1889 "bacillus smegmatis" trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ref OCA], [https://pdbe.org/7ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ref RCSB], [https://www.ebi.ac.uk/pdbsum/7ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ref ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ERS451418_03468 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 "Bacillus smegmatis" Trevisan 1889])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ref OCA], [https://pdbe.org/7ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ref RCSB], [https://www.ebi.ac.uk/pdbsum/7ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ref ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Iron is essential for growth of Mycobacterium tuberculosis, the causative agent of tuberculosis. To acquire iron from the host, M. tuberculosis uses the siderophores called mycobactins and carboxymycobactins. Here, we show that the rv0455c gene is essential for M. tuberculosis to grow in low-iron medium and that secretion of both mycobactins and carboxymycobactins is drastically reduced in the rv0455c deletion mutant. Both water-soluble and membrane-anchored Rv0455c are functional in siderophore secretion, supporting an intracellular role. Lack of Rv0455c results in siderophore toxicity, a phenotype observed for other siderophore secretion mutants, and severely impairs replication of M. tuberculosis in mice, demonstrating the importance of Rv0455c and siderophore secretion during disease. The crystal structure of a Rv0455c homolog reveals a novel protein fold consisting of a helical bundle with a 'cinch' formed by an essential intramolecular disulfide bond. These findings advance our understanding of the distinct M. tuberculosis siderophore secretion system.

			A periplasmic cinched protein is required for siderophore secretion and virulence of Mycobacterium tuberculosis.,Zhang L, Kent JE, Whitaker M, Young DC, Herrmann D, Aleshin AE, Ko YH, Cingolani G, Saad JS, Moody DB, Marassi FM, Ehrt S, Niederweis M Nat Commun. 2022 Apr 26;13(1):2255. doi: 10.1038/s41467-022-29873-6. PMID:35474308<ref>PMID:35474308</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ref" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacillus smegmatis trevisan 1889]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Aleshin AE]]		[[Category: Aleshin, A E]]
	[[Category: Kent JE]]		[[Category: Kent, J E]]
	[[Category: Marassi FM]]		[[Category: Marassi, F M]]
	[[Category: Niederweis M]]		[[Category: Niederweis, M]]
	[[Category: Zhang L]]		[[Category: Zhang, L]]
			[[Category: Mycobacterium smegmatis siderophore secretion]]
			[[Category: Unknown function]]

OCA at 06:09, 18 August 2021

2021-08-18T06:09:20Z

← Older revision		Revision as of 06:09, 18 August 2021
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7ref is ~~ON HOLD~~		==Structure of MS3494 from Mycobacterium smegmatis==
			<StructureSection load='7ref' size='340' side='right'caption='[[7ref]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REF FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ref OCA], [https://pdbe.org/7ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ref RCSB], [https://www.ebi.ac.uk/pdbsum/7ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ref ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Aleshin AE]]
			[[Category: Kent JE]]
			[[Category: Marassi FM]]
			[[Category: Niederweis M]]
			[[Category: Zhang L]]

OCA: Protected "7ref" [edit=sysop:move=sysop]

2021-07-21T08:01:40Z

Protected "7ref" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:01, 21 July 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ref is ON HOLD Authors: Description: Category: Unreleased Structures

2021-07-21T08:01:39Z

New page: '''Unreleased structure''' The entry 7ref is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ref is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]