7rep - Revision history

OCA at 11:37, 30 October 2024

2024-10-30T11:37:43Z

← Older revision		Revision as of 11:37, 30 October 2024
Line 3:		Line 3:
	<StructureSection load='7rep' size='340' side='right'caption='[[7rep]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='7rep' size='340' side='right'caption='[[7rep]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7rep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyvera_cryocrescens Kluyvera cryocrescens].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.192Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.192Å</td></tr>
	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	~~[https://www.uniprot.org/uniprot/PAC_KLUCR PAC_KLUCR]~~

	==See Also==		==See Also==
Line 15:		Line 13:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Kluyvera cryocrescens]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Orth P]]		[[Category: Orth P]]

OCA at 16:29, 18 October 2023

2023-10-18T16:29:55Z

← Older revision		Revision as of 16:29, 18 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7rep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyvera_cryocrescens Kluyvera cryocrescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>		<table><tr><td colspan='2'>[[7rep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyvera_cryocrescens Kluyvera cryocrescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.192Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PAC_KLUCR PAC_KLUCR]]		[https://www.uniprot.org/uniprot/PAC_KLUCR PAC_KLUCR]

	==See Also==		==See Also==

OCA at 07:21, 8 September 2022

2022-09-08T07:21:03Z

← Older revision		Revision as of 07:21, 8 September 2022
Line 3:		Line 3:
	<StructureSection load='7rep' size='340' side='right'caption='[[7rep]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='7rep' size='340' side='right'caption='[[7rep]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7rep]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>		<table><tr><td colspan='2'>[[7rep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyvera_cryocrescens Kluyvera cryocrescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7reo\|7reo]]</div></td></tr>~~
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[[https://www.uniprot.org/uniprot/PAC_KLUCR PAC_KLUCR]]
	The emergence of new therapeutic modalities requires complementary tools for their efficient syntheses. Availability of methodologies for site-selective modification of biomolecules remains a long-standing challenge, given the inherent complexity and the presence of repeating residues that bear functional groups with similar reactivity profiles. We describe a bioconjugation strategy for modification of native peptides relying on high site selectivity conveyed by enzymes. We engineered penicillin G acylases to distinguish among free amino moieties of insulin (two at amino termini and an internal lysine) and manipulate cleavable phenylacetamide groups in a programmable manner to form protected insulin derivatives. This enables selective and specific chemical ligation to synthesize homogeneous bioconjugates, improving yield and purity compared to the existing methods, and generally opens avenues in the functionalization of native proteins to access biological probes or drugs.

	A chemoenzymatic strategy for site-selective functionalization of native peptides and proteins.,Fryszkowska A, An C, Alvizo O, Banerjee G, Canada KA, Cao Y, DeMong D, Devine PN, Duan D, Elgart DM, Farasat I, Gauthier DR, Guidry EN, Jia X, Kong J, Kruse N, Lexa KW, Makarov AA, Mann BF, Milczek EM, Mitchell V, Nazor J, Neri C, Orr RK, Orth P, Phillips EM, Riggins JN, Schafer WA, Silverman SM, Strulson CA, Subramanian N, Voladri R, Yang H, Yang J, Yi X, Zhang X, Zhong W Science. 2022 Jun 17;376(6599):~~1321-1327. doi: 10.1126~~/~~science.abn2009. Epub 2022, Jun 16. PMID:35709255<ref>PMID:35709255</ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 7rep" style="background-color:#fffaf0;"><~~/~~div>~~

	==See Also==		==See Also==
	*[[Penicillin acylase\|Penicillin acylase]]		*[[Penicillin acylase\|Penicillin acylase]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Kluyvera cryocrescens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Penicillin amidase]]~~		[[Category: Orth P]]
	[[Category: Orth, P]]
	~~[[Category: Acylase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Penicillin~~]]

OCA at 16:24, 6 July 2022

2022-07-06T16:24:20Z

← Older revision		Revision as of 16:24, 6 July 2022
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The emergence of new therapeutic modalities requires complementary tools for their efficient syntheses. Availability of methodologies for site-selective modification of biomolecules remains a long-standing challenge, given the inherent complexity and the presence of repeating residues that bear functional groups with similar reactivity profiles. We describe a bioconjugation strategy for modification of native peptides relying on high site selectivity conveyed by enzymes. We engineered penicillin G acylases to distinguish among free amino moieties of insulin (two at amino termini and an internal lysine) and manipulate cleavable phenylacetamide groups in a programmable manner to form protected insulin derivatives. This enables selective and specific chemical ligation to synthesize homogeneous bioconjugates, improving yield and purity compared to the existing methods, and generally opens avenues in the functionalization of native proteins to access biological probes or drugs.

			A chemoenzymatic strategy for site-selective functionalization of native peptides and proteins.,Fryszkowska A, An C, Alvizo O, Banerjee G, Canada KA, Cao Y, DeMong D, Devine PN, Duan D, Elgart DM, Farasat I, Gauthier DR, Guidry EN, Jia X, Kong J, Kruse N, Lexa KW, Makarov AA, Mann BF, Milczek EM, Mitchell V, Nazor J, Neri C, Orr RK, Orth P, Phillips EM, Riggins JN, Schafer WA, Silverman SM, Strulson CA, Subramanian N, Voladri R, Yang H, Yang J, Yi X, Zhang X, Zhong W Science. 2022 Jun 17;376(6599):1321-1327. doi: 10.1126/science.abn2009. Epub 2022, Jun 16. PMID:35709255<ref>PMID:35709255</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7rep" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Penicillin acylase\|Penicillin acylase]]		*[[Penicillin acylase\|Penicillin acylase]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:36, 8 June 2022

2022-06-08T05:36:03Z

← Older revision		Revision as of 05:36, 8 June 2022
Line 1:		Line 1:

	==Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocrescens (A1-Ac Rd3CHis)==		==Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocrescens (A1-Ac Rd3CHis)==
	<StructureSection load='7rep' size='340' side='right'caption='[[7rep]]' scene=''>		<StructureSection load='7rep' size='340' side='right'caption='[[7rep]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>		<table><tr><td colspan='2'>[[7rep]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7reo\|7reo]]</div></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>
	</table>		</table>

			==See Also==
			*[[Penicillin acylase\|Penicillin acylase]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Orth P]]		[[Category: Penicillin amidase]]
			[[Category: Orth, P]]
			[[Category: Acylase]]
			[[Category: Hydrolase]]
			[[Category: Penicillin]]

OCA at 13:39, 17 November 2021

2021-11-17T13:39:26Z

← Older revision		Revision as of 13:39, 17 November 2021
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry 7rep is ON HOLD until Paper Publication~~		==Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocrescens (A1-Ac Rd3CHis)==
			<StructureSection load='7rep' size='340' side='right'caption='[[7rep]]' scene=''>
	~~Authors: Orth, P.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7REP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7REP FirstGlance]. <br>
	~~Description:~~ Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocrescens (A1-Ac Rd3CHis)		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rep OCA], [https://pdbe.org/7rep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rep RCSB], [https://www.ebi.ac.uk/pdbsum/7rep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rep ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Orth, P]]		__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Orth P]]

OCA at 05:06, 18 August 2021

2021-08-18T05:06:05Z

← Older revision		Revision as of 05:06, 18 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7rep is ON HOLD		The entry 7rep is ON HOLD until Paper Publication

	Authors: Orth, P.		Authors: Orth, P.

OCA: Protected "7rep" [edit=sysop:move=sysop]

2021-07-21T08:02:04Z

Protected "7rep" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:02, 21 July 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7rep is ON HOLD Authors: Orth, P. Description: Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocr...

2021-07-21T08:02:03Z

New page: '''Unreleased structure''' The entry 7rep is ON HOLD Authors: Orth, P. Description: Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocr...

New page

'''Unreleased structure'''

The entry 7rep is ON HOLD

Authors: Orth, P.

Description: Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocrescens (A1-Ac Rd3CHis)
[[Category: Unreleased Structures]]
[[Category: Orth, P]]