OCA at 16:33, 18 October 2023

2023-10-18T16:33:02Z

← Older revision		Revision as of 16:33, 18 October 2023
Line 1:		Line 1:

	====		==Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGDRAAGQPA==
	<StructureSection load='7rlw' size='340' side='right'caption='[[7rlw]]' scene=''>		<StructureSection load='7rlw' size='340' side='right'caption='[[7rlw]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7rlw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RLW FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rlw OCA], [https://pdbe.org/7rlw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rlw RCSB], [https://www.ebi.ac.uk/pdbsum/7rlw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rlw ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.54Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rlw OCA], [https://pdbe.org/7rlw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rlw RCSB], [https://www.ebi.ac.uk/pdbsum/7rlw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rlw ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/CSP_PLAVS CSP_PLAVS] Essential sporozoite protein (By similarity). In the mosquito vector, required for sporozoite development in the oocyst, migration through the vector hemolymph and entry into the vector salivary glands (By similarity). In the vertebrate host, required for sporozoite migration through the host dermis and infection of host hepatocytes (By similarity). Binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes (By similarity).[UniProtKB:P02893][UniProtKB:P23093] In the vertebrate host, binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes and is required for sporozoite invasion of the host hepatocytes.[UniProtKB:P23093]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Malaria is a global health burden, with Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) responsible for the majority of infections worldwide. Circumsporozoite protein (CSP) is the most abundant protein on the surface of Plasmodium sporozoites, and antibodies targeting the central repeat region of CSP can prevent parasite infection. Although much has been uncovered about the molecular basis of antibody recognition of the PfCSP repeats, data remains scarce for PvCSP. Here, we performed molecular dynamics simulations for peptides comprising the PvCSP repeats from strains VK210 and VK247 to reveal how the PvCSP central repeats are highly disordered, with minor propensities to adopt turn conformations. Next, we solved eight crystal structures to unveil the interactions of two inhibitory monoclonal antibodies (mAbs), 2F2 and 2E10.E9, with PvCSP repeats. Both antibodies can accommodate subtle sequence variances in the repeat motifs and recognize largely coiled peptide conformations that also contain isolated turns. Our structural studies uncover various degrees of Fab-Fab homotypic interactions upon recognition of the PvCSP central repeats by these two inhibitory mAbs, similar to potent mAbs against PfCSP. These findings augment our understanding of host-Plasmodium interactions and contribute molecular details of Pv inhibition by mAbs to unlock structure-based engineering of PvCSP-based vaccines.

			Structural basis of Plasmodium vivax inhibition by antibodies binding to the circumsporozoite protein repeats.,Kucharska I, Hossain L, Ivanochko D, Yang Q, Rubinstein JL, Pomes R, Julien JP Elife. 2022 Jan 13;11:e72908. doi: 10.7554/eLife.72908. PMID:35023832<ref>PMID:35023832</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7rlw" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Mus musculus]]
			[[Category: Plasmodium vivax]]
			[[Category: Julien JP]]
			[[Category: Kucharska I]]

OCA at 07:03, 27 January 2022

2022-01-27T07:03:38Z

← Older revision		Revision as of 07:03, 27 January 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7rlw is ~~ON HOLD until Paper Publication~~		====
			<StructureSection load='7rlw' size='340' side='right'caption='[[7rlw]]' scene=''>
	~~Authors~~: ~~Kucharska~~, I., ~~Julien~~, J.P.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Antibody 2F2 in complex with P~~. ~~vivax CSP peptide GDRAAGQPAGDRAAGQPA~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rlw OCA], [https://pdbe.org/7rlw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rlw RCSB], [https://www.ebi.ac.uk/pdbsum/7rlw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rlw ProSAT]</span></td></tr>
	[~~[Category~~: ~~Unreleased Structures]~~]		</table>
	[[Category: ~~Kucharska, I~~]]		__TOC__
	[[Category: ~~Julien, J.P~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 12:48, 3 November 2021

2021-11-03T12:48:23Z

← Older revision		Revision as of 12:48, 3 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7rlw is ON HOLD		The entry 7rlw is ON HOLD until Paper Publication

	Authors: Kucharska, I., Julien, J.P.		Authors: Kucharska, I., Julien, J.P.

OCA: Protected "7rlw" [edit=sysop:move=sysop]

2021-08-04T09:21:42Z

Protected "7rlw" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:21, 4 August 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7rlw is ON HOLD Authors: Kucharska, I., Julien, J.P. Description: Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGDRAAGQPA [[Category: ...

2021-08-04T09:21:41Z

New page: '''Unreleased structure''' The entry 7rlw is ON HOLD Authors: Kucharska, I., Julien, J.P. Description: Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGDRAAGQPA [[Category: ...

New page

'''Unreleased structure'''

The entry 7rlw is ON HOLD

Authors: Kucharska, I., Julien, J.P.

Description: Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGDRAAGQPA
[[Category: Unreleased Structures]]
[[Category: Kucharska, I]]
[[Category: Julien, J.P]]

7rlw - Revision history

OCA at 16:33, 18 October 2023

OCA at 07:03, 27 January 2022

OCA at 12:48, 3 November 2021

OCA: Protected "7rlw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7rlw is ON HOLD Authors: Kucharska, I., Julien, J.P. Description: Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGDRAAGQPA [[Category: ...