7rtf - Revision history

OCA at 05:48, 5 June 2024

2024-06-05T05:48:38Z

← Older revision		Revision as of 05:48, 5 June 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7rtf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Spirochaeta_thermophila Spirochaeta thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTF FirstGlance]. <br>		<table><tr><td colspan='2'>[[7rtf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Spirochaeta_thermophila Spirochaeta thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=PGW:(1R)-2-{[(S)-{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(HEXADECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>PGW</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.9Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=PGW:(1R)-2-{[(S)-{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(HEXADECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>PGW</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtf OCA], [https://pdbe.org/7rtf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtf RCSB], [https://www.ebi.ac.uk/pdbsum/7rtf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtf OCA], [https://pdbe.org/7rtf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtf RCSB], [https://www.ebi.ac.uk/pdbsum/7rtf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtf ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/G0GA88_SPITZ G0GA88_SPITZ]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Understanding how ion channels gate is important for elucidating their physiological roles and targeting them in pathophysiological states. Here, we used SthK, a cyclic nucleotide-modulated channel from Spirochaeta thermophila, to define a ligand-gating trajectory that includes multiple on-pathway intermediates. cAMP is a poor partial agonist for SthK and depolarization increases SthK activity. Tuning the energy landscape by gain-of-function mutations in the voltage sensor domain (VSD) allowed us to capture multiple intermediates along the ligand-activation pathway, highlighting the allosteric linkage between VSD, cyclic nucleotide-binding (CNBD) and pore domains. Small, lateral displacements of the VSD S4 segment were necessary to open the intracellular gate, pointing to an inhibitory VSD at rest. We propose that in wild-type SthK, depolarization leads to such VSD displacements resulting in release of inhibition. In summary, we report conformational transitions along the activation pathway that reveal allosteric couplings between key sites integrating to open the intracellular gate.

			Gating intermediates reveal inhibitory role of the voltage sensor in a cyclic nucleotide-modulated ion channel.,Gao X, Schmidpeter PAM, Berka V, Durham RJ, Fan C, Jayaraman V, Nimigean CM Nat Commun. 2022 Nov 14;13(1):6919. doi: 10.1038/s41467-022-34673-z. PMID:36376326<ref>PMID:36376326</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7rtf" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:03, 24 November 2022

2022-11-24T10:03:16Z

← Older revision		Revision as of 10:03, 24 November 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7rtf is ~~ON HOLD until Paper Publication~~		==SthK R120A Closed State==
			<StructureSection load='7rtf' size='340' side='right'caption='[[7rtf]], [[Resolution\|resolution]] 2.90Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[7rtf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Spirochaeta_thermophila Spirochaeta thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTF FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=PGW:(1R)-2-{[(S)-{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(HEXADECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>PGW</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtf OCA], [https://pdbe.org/7rtf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtf RCSB], [https://www.ebi.ac.uk/pdbsum/7rtf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtf ProSAT]</span></td></tr>
			</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Spirochaeta thermophila]]
			[[Category: Gao X]]
			[[Category: Nimigean C]]

OCA at 03:40, 26 October 2022

2022-10-26T03:40:41Z

← Older revision		Revision as of 03:40, 26 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7rtf is ON HOLD		The entry 7rtf is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:23, 28 September 2022

2022-09-28T04:23:26Z

← Older revision		Revision as of 04:23, 28 September 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7rtf is ON HOLD ~~until Paper Publication~~		The entry 7rtf is ON HOLD

	Authors:		Authors:

OCA: Protected "7rtf" [edit=sysop:move=sysop]

2021-08-25T05:17:25Z

Protected "7rtf" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:17, 25 August 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7rtf is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2021-08-25T05:17:24Z

New page: '''Unreleased structure''' The entry 7rtf is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7rtf is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]