7sci - Revision history

OCA at 16:49, 18 October 2023

2023-10-18T16:49:28Z

← Older revision		Revision as of 16:49, 18 October 2023
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	<StructureSection load='7sci' size='340' side='right'caption='[[7sci]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='7sci' size='340' side='right'caption='[[7sci]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7sci]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SCI FirstGlance]. <br>		<table><tr><td colspan='2'>[[7sci]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Akkermansia_muciniphila_ATCC_BAA-835 Akkermansia muciniphila ATCC BAA-835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SCI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sci OCA], [https://pdbe.org/7sci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sci RCSB], [https://www.ebi.ac.uk/pdbsum/7sci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sci ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sci OCA], [https://pdbe.org/7sci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sci RCSB], [https://www.ebi.ac.uk/pdbsum/7sci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sci ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/B2UPI7_AKKM8 B2UPI7_AKKM8]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Akkermansia muciniphila ATCC BAA-835]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bertozzi~~, C R~~]]		[[Category: Bertozzi CR]]
	[[Category: Fernandez, D]]		[[Category: Fernandez D]]
	[[Category: Shon~~, D J]]~~		[[Category: Shon DJ]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Metalloenzyme]]~~
	~~[[Category: Mucinase]]~~
	~~[[Category: O-glycoprotease~~]]

OCA at 08:16, 25 May 2022

2022-05-25T08:16:10Z

← Older revision		Revision as of 08:16, 25 May 2022
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	==AM0627 metallopeptidase from Akkermansia muciniphila==		==AM0627 metallopeptidase from Akkermansia muciniphila==
	<StructureSection load='7sci' size='340' side='right'caption='[[7sci]]' scene=''>		<StructureSection load='7sci' size='340' side='right'caption='[[7sci]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SCI FirstGlance]. <br>		<table><tr><td colspan='2'>[[7sci]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SCI FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sci OCA], [https://pdbe.org/7sci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sci RCSB], [https://www.ebi.ac.uk/pdbsum/7sci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sci ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sci OCA], [https://pdbe.org/7sci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sci RCSB], [https://www.ebi.ac.uk/pdbsum/7sci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sci ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Akkermansia muciniphila, a mucin-degrading microbe found in the human gut, is often associated with positive health outcomes. The abundance of Akkermansia muciniphila is modulated by the presence and accessibility of nutrients, which can be derived from diet or host glycoproteins. In particular, the ability to degrade host mucins, a class of proteins carrying densely O-glycosylated domains, provides a competitive advantage in the sustained colonization of niche mucosal environments. Although Akkermansia muciniphila is known to rely on mucins as a carbon and nitrogen source, the enzymatic machinery used by this microbe to process mucins in the gut is not yet fully characterized. Here, we focus on the mucin-selective metalloprotease, Amuc_0627 (AM0627), which is known to cleave between adjacent residues carrying truncated core 1 O-glycans. We showed that this enzyme is capable of degrading purified mucin 2 (MUC2), the major protein component of mucus in the gut. An X-ray crystal structure of AM0627 (1.9 A resolution) revealed O-glycan binding residues that are conserved between structurally characterized enzymes from the same family. We further rationalized the substrate cleavage motif using molecular modeling to identify nonconserved glycan-interacting residues. We conclude that mutagenesis of these residues resulted in altered substrate preferences down to the glycan level, providing insight into the structural determinants of O-glycan recognition.

			Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences.,Shon DJ, Fernandez D, Riley NM, Ferracane MJ, Bertozzi CR J Biol Chem. 2022 Apr 8:101917. doi: 10.1016/j.jbc.2022.101917. PMID:35405095<ref>PMID:35405095</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7sci" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bertozzi CR]]		[[Category: Bertozzi, C R]]
	[[Category: Fernandez D]]		[[Category: Fernandez, D]]
	[[Category: Shon DJ]]		[[Category: Shon, D J]]
			[[Category: Hydrolase]]
			[[Category: Metalloenzyme]]
			[[Category: Mucinase]]
			[[Category: O-glycoprotease]]

OCA at 11:39, 27 April 2022

2022-04-27T11:39:27Z

← Older revision		Revision as of 11:39, 27 April 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7sci is ~~ON HOLD until Paper Publication~~		==AM0627 metallopeptidase from Akkermansia muciniphila==
			<StructureSection load='7sci' size='340' side='right'caption='[[7sci]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SCI FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sci OCA], [https://pdbe.org/7sci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sci RCSB], [https://www.ebi.ac.uk/pdbsum/7sci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sci ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Bertozzi CR]]
			[[Category: Fernandez D]]
			[[Category: Shon DJ]]

OCA at 12:49, 3 November 2021

2021-11-03T12:49:35Z

← Older revision		Revision as of 12:49, 3 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7sci is ON HOLD		The entry 7sci is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7sci" [edit=sysop:move=sysop]

2021-10-06T04:16:15Z

Protected "7sci" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:16, 6 October 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7sci is ON HOLD Authors: Description: Category: Unreleased Structures

2021-10-06T04:16:14Z

New page: '''Unreleased structure''' The entry 7sci is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7sci is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]