OCA at 16:49, 18 October 2023

2023-10-18T16:49:40Z

← Older revision		Revision as of 16:49, 18 October 2023
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	====		==Murine Fab that recognizes Hev b 8 (profilin for Hevea brasiliensis)==
	<StructureSection load='7sd2' size='340' side='right'caption='[[7sd2]]' scene=''>		<StructureSection load='7sd2' size='340' side='right'caption='[[7sd2]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7sd2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SD2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sd2 OCA], [https://pdbe.org/7sd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sd2 RCSB], [https://www.ebi.ac.uk/pdbsum/7sd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sd2 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.75Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sd2 OCA], [https://pdbe.org/7sd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sd2 RCSB], [https://www.ebi.ac.uk/pdbsum/7sd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sd2 ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Allergies have become a rising health problem, where plentiful substances can trigger IgE-mediated allergies in humans. While profilins are considered minor allergens, these ubiquitous proteins are primary molecules involved in cross-reactivity and pollen-food allergy syndrome. Here we report the first crystal structures of murine Fab/IgE, with its chains naturally paired, in complex with the allergen profilin from Hevea brasiliensis (Hev b 8). The crystallographic models revealed that the IgE's six complementarity-determining regions (CDRs) interact with the allergen, comprising a rigid paratope-epitope surface of 926 A(2), which includes an extensive network of interactions. Interestingly, we also observed previously unreported flexibility at Fab/IgE's elbow angle, which did not influence the shape of the paratope. The Fab/IgE exhibits a high affinity for Hev b 8, even when using 1 M NaCl in BLI experiments. Finally, based on the encouraging cross-reactivity assays using two mutants of the maize profilin (Zea m 12), this antibody could be a promising tool in IgE engineering for diagnosis and research applications.

			A native IgE in complex with profilin provides insights into allergen recognition and cross-reactivity.,Garcia-Ramirez B, Mares-Mejia I, Rodriguez-Hernandez A, Cano-Sanchez P, Torres-Larios A, Ortega E, Rodriguez-Romero A Commun Biol. 2022 Jul 27;5(1):748. doi: 10.1038/s42003-022-03718-w. PMID:35902770<ref>PMID:35902770</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7sd2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Antibody 3D structures\|Antibody 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Z-~~disk~~]]		[[Category: Mus musculus]]
			[[Category: Garcia-Ramirez B]]
			[[Category: Rodriguez-Romero A]]

OCA at 05:00, 10 August 2022

2022-08-10T05:00:09Z

← Older revision		Revision as of 05:00, 10 August 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7sd2 is ~~ON HOLD until Paper Publication~~		====
			<StructureSection load='7sd2' size='340' side='right'caption='[[7sd2]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sd2 OCA], [https://pdbe.org/7sd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sd2 RCSB], [https://www.ebi.ac.uk/pdbsum/7sd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sd2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 12:49, 3 November 2021

2021-11-03T12:49:56Z

← Older revision		Revision as of 12:49, 3 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7sd2 is ON HOLD		The entry 7sd2 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7sd2" [edit=sysop:move=sysop]

2021-10-06T04:17:10Z

Protected "7sd2" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:17, 6 October 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7sd2 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-10-06T04:17:09Z

New page: '''Unreleased structure''' The entry 7sd2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7sd2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7sd2 - Revision history

OCA at 16:49, 18 October 2023

OCA at 05:00, 10 August 2022

OCA at 12:49, 3 November 2021

OCA: Protected "7sd2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7sd2 is ON HOLD Authors: Description: Category: Unreleased Structures