7sim - Revision history

OCA at 14:00, 6 November 2024

2024-11-06T14:00:54Z

← Older revision		Revision as of 14:00, 6 November 2024
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	==Structure of positive allosteric modulator-free active human calcium-sensing receptor==		==Structure of positive allosteric modulator-free active human calcium-sensing receptor==
	<StructureSection load='7sim' size='340' side='right'caption='[[7sim]]' scene=''>		<StructureSection load='7sim' size='340' side='right'caption='[[7sim]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SIM FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SIM FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sim OCA], [https://pdbe.org/7sim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sim RCSB], [https://www.ebi.ac.uk/pdbsum/7sim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sim ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.7Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TCR:CYCLOMETHYLTRYPTOPHAN'>TCR</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sim OCA], [https://pdbe.org/7sim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sim RCSB], [https://www.ebi.ac.uk/pdbsum/7sim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sim ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The human extracellular calcium-sensing (CaS) receptor controls plasma Ca(2+) levels and contributes to nutrient-dependent maintenance and metabolism of diverse organs. Allosteric modulation of the CaS receptor corrects disorders of calcium homeostasis. Here, we report the cryogenic-electron microscopy reconstructions of a near-full-length CaS receptor in the absence and presence of allosteric modulators. Activation of the homodimeric CaS receptor requires a break in the transmembrane 6 (TM6) helix of each subunit, which facilitates the formation of a TM6-mediated homodimer interface and expansion of homodimer interactions. This transformation in TM6 occurs without a positive allosteric modulator. Two modulators with opposite functional roles bind to overlapping sites within the transmembrane domain through common interactions, acting to stabilize distinct rotamer conformations of key residues on the TM6 helix. The positive modulator reinforces TM6 distortion and maximizes subunit contact to enhance receptor activity, while the negative modulator strengthens an intact TM6 to dampen receptor function. In both active and inactive states, the receptor displays symmetrical transmembrane conformations that are consistent with its homodimeric assembly.

			Symmetric activation and modulation of the human calcium-sensing receptor.,Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR Proc Natl Acad Sci U S A. 2021 Dec 21;118(51). pii: 2115849118. doi:, 10.1073/pnas.2115849118. PMID:34916296<ref>PMID:34916296</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7sim" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:31, 19 January 2022

2022-01-19T07:31:38Z

@@ Line 1: / Line 1: @@
-The entry 7sim is ON HOLD  until Paper Publication
+==Structure of positive allosteric modulator-free active human calcium-sensing receptor==
+<StructureSection load='7sim' size='340' side='right'caption='[[7sim]]' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SIM FirstGlance]. <br>
-Description:
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sim OCA], [https://pdbe.org/7sim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sim RCSB], [https://www.ebi.ac.uk/pdbsum/7sim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sim ProSAT]</span></td></tr>
-[[Category: Unreleased Structures]]
+</table>

OCA at 12:51, 3 November 2021

2021-11-03T12:51:10Z

← Older revision		Revision as of 12:51, 3 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7sim is ON HOLD		The entry 7sim is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7sim" [edit=sysop:move=sysop]

2021-10-20T15:10:17Z

Protected "7sim" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:10, 20 October 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7sim is ON HOLD Authors: Description: Category: Unreleased Structures

2021-10-20T15:10:16Z

New page: '''Unreleased structure''' The entry 7sim is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7sim is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]