7sov - Revision history

OCA at 16:53, 18 October 2023

2023-10-18T16:53:50Z

← Older revision		Revision as of 16:53, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7sov]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SOV FirstGlance]. <br>		<table><tr><td colspan='2'>[[7sov]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SOV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sov OCA], [https://pdbe.org/7sov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sov RCSB], [https://www.ebi.ac.uk/pdbsum/7sov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sov ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sov OCA], [https://pdbe.org/7sov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sov RCSB], [https://www.ebi.ac.uk/pdbsum/7sov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sov ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:25, 24 November 2022

2022-11-24T10:25:16Z

← Older revision		Revision as of 10:25, 24 November 2022
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	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/LARP1_HUMAN LARP1_HUMAN]] RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. Associates with the mRNA 5'cap in an MTOR-dependent manner and associates with mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding for ribosomal proteins and several components of the translation machinery. Associates with actively translating ribosomes via interaction with PABPC1/PABP and stimulates translation of mRNAs containing a 5'TOP, thereby regulating cell growth and proliferation.<ref>PMID:20430826</ref> <ref>PMID:23711370</ref> <ref>PMID:24532714</ref>		[https://www.uniprot.org/uniprot/LARP1_HUMAN LARP1_HUMAN] RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. Associates with the mRNA 5'cap in an MTOR-dependent manner and associates with mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding for ribosomal proteins and several components of the translation machinery. Associates with actively translating ribosomes via interaction with PABPC1/PABP and stimulates translation of mRNAs containing a 5'TOP, thereby regulating cell growth and proliferation.<ref>PMID:20430826</ref> <ref>PMID:23711370</ref> <ref>PMID:24532714</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:48, 7 September 2022

2022-09-07T19:48:47Z

← Older revision		Revision as of 19:48, 7 September 2022
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	====		==LaM domain of human LARP1 in complex with AAAAAAAAAAA RNA polynucleotide==
	<StructureSection load='7sov' size='340' side='right'caption='[[7sov]]' scene=''>		<StructureSection load='7sov' size='340' side='right'caption='[[7sov]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7sov]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SOV FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sov OCA], [https://pdbe.org/7sov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sov RCSB], [https://www.ebi.ac.uk/pdbsum/7sov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sov ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sov OCA], [https://pdbe.org/7sov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sov RCSB], [https://www.ebi.ac.uk/pdbsum/7sov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sov ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/LARP1_HUMAN LARP1_HUMAN]] RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. Associates with the mRNA 5'cap in an MTOR-dependent manner and associates with mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding for ribosomal proteins and several components of the translation machinery. Associates with actively translating ribosomes via interaction with PABPC1/PABP and stimulates translation of mRNAs containing a 5'TOP, thereby regulating cell growth and proliferation.<ref>PMID:20430826</ref> <ref>PMID:23711370</ref> <ref>PMID:24532714</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			La-related proteins (LARPs) comprise a family of RNA-binding proteins involved in a wide range of posttranscriptional regulatory activities. LARPs share a unique tandem of two RNA-binding domains, La motif (LaM) and RNA recognition motif (RRM), together referred to as a La-module, but vary in member-specific regions. Prior structural studies of La-modules reveal they are pliable platforms for RNA recognition in diverse contexts. Here, we characterize the La-module of LARP1, which plays an important role in regulating synthesis of ribosomal proteins in response to mTOR signaling and mRNA stabilization. LARP1 has been well characterized functionally but no structural information exists for its La-module. We show that unlike other LARPs, the La-module in LARP1 does not contain an RRM domain. The LaM alone is sufficient for binding poly(A) RNA with submicromolar affinity and specificity. Multiple high-resolution crystal structures of the LARP1 LaM domain in complex with poly(A) show that it is highly specific for the RNA 3'-end, and identify LaM residues Q333, Y336 and F348 as the most critical for binding. Use of a quantitative mRNA stabilization assay and poly(A) tail-sequencing demonstrate functional relevance of LARP1 RNA binding in cells and provide novel insight into its poly(A) 3' protection activity.

			Structural basis of 3'-end poly(A) RNA recognition by LARP1.,Kozlov G, Mattijssen S, Jiang J, Nyandwi S, Sprules T, Iben JR, Coon SL, Gaidamakov S, Noronha AM, Wilds CJ, Maraia RJ, Gehring K Nucleic Acids Res. 2022 Aug 18. pii: 6671114. doi: 10.1093/nar/gkac696. PMID:35979957<ref>PMID:35979957</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7sov" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Synthetic construct]]
			[[Category: Gehring K]]
			[[Category: Kozlov G]]

OCA at 04:53, 3 August 2022

2022-08-03T04:53:20Z

← Older revision		Revision as of 04:53, 3 August 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7sov is ~~ON HOLD until Paper Publication~~		====
			<StructureSection load='7sov' size='340' side='right'caption='[[7sov]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sov OCA], [https://pdbe.org/7sov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sov RCSB], [https://www.ebi.ac.uk/pdbsum/7sov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sov ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 10:56, 24 November 2021

2021-11-24T10:56:37Z

← Older revision		Revision as of 10:56, 24 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7sov is ON HOLD		The entry 7sov is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7sov" [edit=sysop:move=sysop]

2021-11-10T05:17:24Z

Protected "7sov" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:17, 10 November 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7sov is ON HOLD Authors: Description: Category: Unreleased Structures

2021-11-10T05:17:23Z

New page: '''Unreleased structure''' The entry 7sov is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7sov is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]