7ssl - Revision history

OCA at 09:11, 17 October 2024

2024-10-17T09:11:19Z

← Older revision		Revision as of 09:11, 17 October 2024
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	====		==Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III)==
	<StructureSection load='7ssl' size='340' side='right'caption='[[7ssl]]' scene=''>		<StructureSection load='7ssl' size='340' side='right'caption='[[7ssl]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ssl]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SSL FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ssl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ssl OCA], [https://pdbe.org/7ssl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ssl RCSB], [https://www.ebi.ac.uk/pdbsum/7ssl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ssl ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ssl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ssl OCA], [https://pdbe.org/7ssl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ssl RCSB], [https://www.ebi.ac.uk/pdbsum/7ssl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ssl ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			During translation, a conserved GTPase elongation factor-EF-G in bacteria or eEF2 in eukaryotes-translocates tRNA and mRNA through the ribosome. EF-G has been proposed to act as a flexible motor that propels tRNA and mRNA movement, as a rigid pawl that biases unidirectional translocation resulting from ribosome rearrangements, or by various combinations of motor- and pawl-like mechanisms. Using time-resolved cryo-EM, we visualized GTP-catalyzed translocation without inhibitors, capturing elusive structures of ribosome*EF-G intermediates at near-atomic resolution. Prior to translocation, EF-G binds near peptidyl-tRNA, while the rotated 30S subunit stabilizes the EF-G GTPase center. Reverse 30S rotation releases Pi and translocates peptidyl-tRNA and EF-G by ~20 A. An additional 4-A translocation initiates EF-G dissociation from a transient ribosome state with highly swiveled 30S head. The structures visualize how nearly rigid EF-G rectifies inherent and spontaneous ribosomal dynamics into tRNA-mRNA translocation, whereas GTP hydrolysis and Pi release drive EF-G dissociation.

			Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP.,Carbone CE, Loveland AB, Gamper HB Jr, Hou YM, Demo G, Korostelev AA Nat Commun. 2021 Dec 13;12(1):7236. doi: 10.1038/s41467-021-27415-0. PMID:34903725<ref>PMID:34903725</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ssl" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Carbone CE]]
			[[Category: Korostelev AA]]

OCA at 07:44, 23 February 2022

2022-02-23T07:44:15Z

← Older revision		Revision as of 07:44, 23 February 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7ssl ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7ssl' size='340' side='right'caption='[[7ssl]]' scene=''>
	~~Authors~~: ~~Carbone, C~~.E.~~, Korostelev, A~~.A.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Pre translocation intermediate with EF~~-~~G bound to GDP and Pi (Structure III)~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ssl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ssl OCA], [https://pdbe.org/7ssl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ssl RCSB], [https://www.ebi.ac.uk/pdbsum/7ssl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ssl ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[Category: ~~Carbone, C.E~~]]		__TOC__
	[[Category: ~~Korostelev, A.A~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 06:14, 9 February 2022

2022-02-09T06:14:58Z

← Older revision		Revision as of 06:14, 9 February 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7ssl is ON HOLD		The entry 7ssl is ON HOLD until Paper Publication

	Authors: Carbone, C.E., Korostelev, A.A.		Authors: Carbone, C.E., Korostelev, A.A.

OCA: Protected "7ssl" [edit=sysop:move=sysop]

2021-11-17T12:16:55Z

Protected "7ssl" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:16, 17 November 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ssl is ON HOLD Authors: Carbone, C.E., Korostelev, A.A. Description: Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III) ...

2021-11-17T12:16:54Z

New page: '''Unreleased structure''' The entry 7ssl is ON HOLD Authors: Carbone, C.E., Korostelev, A.A. Description: Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III) ...

New page

'''Unreleased structure'''

The entry 7ssl is ON HOLD

Authors: Carbone, C.E., Korostelev, A.A.

Description: Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III)
[[Category: Unreleased Structures]]
[[Category: Carbone, C.E]]
[[Category: Korostelev, A.A]]