7swt - Revision history

OCA at 08:35, 19 March 2026

2026-03-19T08:35:51Z

← Older revision		Revision as of 08:35, 19 March 2026
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	<StructureSection load='7swt' size='340' side='right'caption='[[7swt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='7swt' size='340' side='right'caption='[[7swt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7swt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinopora_forskaliana Echinopora forskaliana].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWT FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWT FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.005Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.005Å</td></tr>
	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swt OCA], [https://pdbe.org/7swt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swt RCSB], [https://www.ebi.ac.uk/pdbsum/7swt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swt OCA], [https://pdbe.org/7swt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swt RCSB], [https://www.ebi.ac.uk/pdbsum/7swt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swt ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	~~[https://www.uniprot.org/uniprot/B6CTZ7_9CNID B6CTZ7_9CNID]~~
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Anthozoan chromoproteins are highly pigmented, diversely coloured and readily produced in recombinant expression systems. While they are a versatile and powerful building block in synthetic biology for applications such as biosensor development, they are not widely used in comparison to the related fluorescent proteins, partly due to a lack of structural characterization to aid protein engineering. Here, high-resolution X-ray crystal structures of four open-source chromoproteins, gfasPurple, amilCP, spisPink and eforRed, are presented. These proteins are dimers in solution, and mutation at the conserved dimer interface leads to loss of visible colour development in gfasPurple. The chromophores are trans and noncoplanar in gfasPurple, amilCP and spisPink, while that in eforRed is cis and noncoplanar, and also emits fluorescence. Like other characterized chromoproteins, gfasPurple, amilCP and eforRed contain an sp(2)-hybridized N-acylimine in the peptide bond preceding the chromophore, while spisPink is unusual and demonstrates a true sp(3)-hybridized trans-peptide bond at this position. It was found that point mutations at the chromophore-binding site in gfasPurple that substitute similar amino acids to those in amilCP and spisPink generate similar colours. These features and observations have implications for the utility of these chromoproteins in protein engineering and synthetic biology applications.

	Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed.,Ahmed FH, Caputo AT, French NG, Peat TS, Whitfield J, Warden AC, Newman J, Scott C Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):599-612. doi:, 10.1107/S2059798322002625. Epub 2022 Apr 8. PMID:35503208<ref>PMID:35503208</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7swt" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Echinopora forskaliana]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ahmed H]]		[[Category: Ahmed H]]

OCA at 16:55, 18 October 2023

2023-10-18T16:55:58Z

← Older revision		Revision as of 16:55, 18 October 2023
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	<StructureSection load='7swt' size='340' side='right'caption='[[7swt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='7swt' size='340' side='right'caption='[[7swt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7swt]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWT FirstGlance]. <br>		<table><tr><td colspan='2'>[[7swt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinopora_forskaliana Echinopora forskaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWT FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.005Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swt OCA], [https://pdbe.org/7swt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swt RCSB], [https://www.ebi.ac.uk/pdbsum/7swt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swt OCA], [https://pdbe.org/7swt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swt RCSB], [https://www.ebi.ac.uk/pdbsum/7swt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swt ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/B6CTZ7_9CNID B6CTZ7_9CNID]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Echinopora forskaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ahmed, H]]		[[Category: Ahmed H]]
	[[Category: Caputo~~, A T~~]]		[[Category: Caputo AT]]
	[[Category: Newman, J]]		[[Category: Newman J]]
	[[Category: Scott, C]]		[[Category: Scott C]]
	~~[[Category: Chromoprotein]]~~
	~~[[Category: Fluorescent protein~~]]

OCA at 10:26, 18 May 2022

2022-05-18T10:26:32Z

← Older revision		Revision as of 10:26, 18 May 2022
Line 1:		Line 1:

	==Crystal structure of the chromoprotein eforRED==		==Crystal structure of the chromoprotein eforRED==
	<StructureSection load='7swt' size='340' side='right'caption='[[7swt]]' scene=''>		<StructureSection load='7swt' size='340' side='right'caption='[[7swt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWT FirstGlance]. <br>		<table><tr><td colspan='2'>[[7swt]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWT FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swt OCA], [https://pdbe.org/7swt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swt RCSB], [https://www.ebi.ac.uk/pdbsum/7swt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swt ProSAT]</span></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swt OCA], [https://pdbe.org/7swt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swt RCSB], [https://www.ebi.ac.uk/pdbsum/7swt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swt ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Anthozoan chromoproteins are highly pigmented, diversely coloured and readily produced in recombinant expression systems. While they are a versatile and powerful building block in synthetic biology for applications such as biosensor development, they are not widely used in comparison to the related fluorescent proteins, partly due to a lack of structural characterization to aid protein engineering. Here, high-resolution X-ray crystal structures of four open-source chromoproteins, gfasPurple, amilCP, spisPink and eforRed, are presented. These proteins are dimers in solution, and mutation at the conserved dimer interface leads to loss of visible colour development in gfasPurple. The chromophores are trans and noncoplanar in gfasPurple, amilCP and spisPink, while that in eforRed is cis and noncoplanar, and also emits fluorescence. Like other characterized chromoproteins, gfasPurple, amilCP and eforRed contain an sp(2)-hybridized N-acylimine in the peptide bond preceding the chromophore, while spisPink is unusual and demonstrates a true sp(3)-hybridized trans-peptide bond at this position. It was found that point mutations at the chromophore-binding site in gfasPurple that substitute similar amino acids to those in amilCP and spisPink generate similar colours. These features and observations have implications for the utility of these chromoproteins in protein engineering and synthetic biology applications.

			Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed.,Ahmed FH, Caputo AT, French NG, Peat TS, Whitfield J, Warden AC, Newman J, Scott C Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):599-612. doi:, 10.1107/S2059798322002625. Epub 2022 Apr 8. PMID:35503208<ref>PMID:35503208</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7swt" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ahmed H]]		[[Category: Ahmed, H]]
	[[Category: Caputo AT]]		[[Category: Caputo, A T]]
	[[Category: Newman J]]		[[Category: Newman, J]]
	[[Category: Scott C]]		[[Category: Scott, C]]
			[[Category: Chromoprotein]]
			[[Category: Fluorescent protein]]

OCA at 02:56, 21 April 2022

2022-04-21T02:56:19Z

← Older revision		Revision as of 02:56, 21 April 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7swt is ~~ON HOLD until Paper Publication~~		==Crystal structure of the chromoprotein eforRED==
			<StructureSection load='7swt' size='340' side='right'caption='[[7swt]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWT FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swt OCA], [https://pdbe.org/7swt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swt RCSB], [https://www.ebi.ac.uk/pdbsum/7swt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swt ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Ahmed H]]
			[[Category: Caputo AT]]
			[[Category: Newman J]]
			[[Category: Scott C]]

OCA at 05:54, 19 January 2022

2022-01-19T05:54:32Z

← Older revision		Revision as of 05:54, 19 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7swt is ON HOLD		The entry 7swt is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 12:01, 29 December 2021

2021-12-29T12:01:07Z

← Older revision		Revision as of 12:01, 29 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7swt is ON HOLD ~~until Paper Publication~~		The entry 7swt is ON HOLD

	Authors:		Authors:

OCA at 15:40, 15 December 2021

2021-12-15T15:40:36Z

← Older revision		Revision as of 15:40, 15 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7swt is ON HOLD		The entry 7swt is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7swt" [edit=sysop:move=sysop]

2021-12-01T05:13:11Z

Protected "7swt" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:13, 1 December 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7swt is ON HOLD Authors: Description: Category: Unreleased Structures

2021-12-01T05:13:10Z

New page: '''Unreleased structure''' The entry 7swt is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7swt is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]