7t4e - Revision history

OCA at 13:06, 1 March 2024

2024-03-01T13:06:52Z

← Older revision		Revision as of 13:06, 1 March 2024
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	<StructureSection load='7t4e' size='340' side='right'caption='[[7t4e]], [[Resolution\|resolution]] 2.87Å' scene=''>		<StructureSection load='7t4e' size='340' side='right'caption='[[7t4e]], [[Resolution\|resolution]] 2.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7t4e]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[7t4e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4E FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4e OCA], [https://pdbe.org/7t4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4e RCSB], [https://www.ebi.ac.uk/pdbsum/7t4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4e ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.87Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4e OCA], [https://pdbe.org/7t4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4e RCSB], [https://www.ebi.ac.uk/pdbsum/7t4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4e ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Enterococci are a part of human microbiota and a leading cause of multidrug resistant infections. Here, we identify a family of Enterococcus pore-forming toxins (Epxs) in E. faecalis, E. faecium, and E. hirae strains isolated across the globe. Structural studies reveal that Epxs form a branch of beta-barrel pore-forming toxins with a beta-barrel protrusion (designated the top domain) sitting atop the cap domain. Through a genome-wide CRISPR-Cas9 screen, we identify human leukocyte antigen class I (HLA-I) complex as a receptor for two members (Epx2 and Epx3), which preferentially recognize human HLA-I and homologous MHC-I of equine, bovine, and porcine, but not murine, origin. Interferon exposure, which stimulates MHC-I expression, sensitizes human cells and intestinal organoids to Epx2 and Epx3 toxicity. Co-culture with Epx2-harboring E. faecium damages human peripheral blood mononuclear cells and intestinal organoids, and this toxicity is neutralized by an Epx2 antibody, demonstrating the toxin-mediated virulence of Epx-carrying Enterococcus.

	Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors.,Xiong X, Tian S, Yang P, Lebreton F, Bao H, Sheng K, Yin L, Chen P, Zhang J, Qi W, Ruan J, Wu H, Chen H, Breault DT, Wu H, Earl AM, Gilmore MS, Abraham J, Dong M Cell. 2022 Mar 2. pii: S0092-8674(22)00142-8. doi: 10.1016/j.cell.2022.02.002. PMID:35259335<ref>PMID:35259335</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7t4e" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Enterococcus faecalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abraham, J]]		[[Category: Abraham J]]
	[[Category: Dong, M]]		[[Category: Dong M]]
	[[Category: Xiong~~, X Z~~]]		[[Category: Xiong XZ]]
	[[Category: Yang, P]]		[[Category: Yang P]]
	~~[[Category: Pore-forming toxin]]~~
	~~[[Category: Toxin~~]]

OCA at 12:01, 23 March 2022

2022-03-23T12:01:34Z

← Older revision		Revision as of 12:01, 23 March 2022
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	==Prepore structure of pore-forming toxin Epx1==		==Prepore structure of pore-forming toxin Epx1==
	<StructureSection load='7t4e' size='340' side='right'caption='[[7t4e]]' scene=''>		<StructureSection load='7t4e' size='340' side='right'caption='[[7t4e]], [[Resolution\|resolution]] 2.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[7t4e]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4E FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4e OCA], [https://pdbe.org/7t4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4e RCSB], [https://www.ebi.ac.uk/pdbsum/7t4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4e ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4e OCA], [https://pdbe.org/7t4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4e RCSB], [https://www.ebi.ac.uk/pdbsum/7t4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4e ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Enterococci are a part of human microbiota and a leading cause of multidrug resistant infections. Here, we identify a family of Enterococcus pore-forming toxins (Epxs) in E. faecalis, E. faecium, and E. hirae strains isolated across the globe. Structural studies reveal that Epxs form a branch of beta-barrel pore-forming toxins with a beta-barrel protrusion (designated the top domain) sitting atop the cap domain. Through a genome-wide CRISPR-Cas9 screen, we identify human leukocyte antigen class I (HLA-I) complex as a receptor for two members (Epx2 and Epx3), which preferentially recognize human HLA-I and homologous MHC-I of equine, bovine, and porcine, but not murine, origin. Interferon exposure, which stimulates MHC-I expression, sensitizes human cells and intestinal organoids to Epx2 and Epx3 toxicity. Co-culture with Epx2-harboring E. faecium damages human peripheral blood mononuclear cells and intestinal organoids, and this toxicity is neutralized by an Epx2 antibody, demonstrating the toxin-mediated virulence of Epx-carrying Enterococcus.

			Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors.,Xiong X, Tian S, Yang P, Lebreton F, Bao H, Sheng K, Yin L, Chen P, Zhang J, Qi W, Ruan J, Wu H, Chen H, Breault DT, Wu H, Earl AM, Gilmore MS, Abraham J, Dong M Cell. 2022 Mar 2. pii: S0092-8674(22)00142-8. doi: 10.1016/j.cell.2022.02.002. PMID:35259335<ref>PMID:35259335</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7t4e" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abraham J]]		[[Category: Abraham, J]]
	[[Category: Dong M]]		[[Category: Dong, M]]
	[[Category: Xiong XZ]]		[[Category: Xiong, X Z]]
	[[Category: Yang P]]		[[Category: Yang, P]]
			[[Category: Pore-forming toxin]]
			[[Category: Toxin]]

OCA at 06:17, 16 March 2022

2022-03-16T06:17:38Z

← Older revision		Revision as of 06:17, 16 March 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7t4e is ~~ON HOLD~~		==Prepore structure of pore-forming toxin Epx1==
			<StructureSection load='7t4e' size='340' side='right'caption='[[7t4e]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4E FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4e OCA], [https://pdbe.org/7t4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4e RCSB], [https://www.ebi.ac.uk/pdbsum/7t4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4e ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Abraham J]]
			[[Category: Dong M]]
			[[Category: Xiong XZ]]
			[[Category: Yang P]]

OCA: Protected "7t4e" [edit=sysop:move=sysop]

2021-12-15T15:45:37Z

Protected "7t4e" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:45, 15 December 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7t4e is ON HOLD Authors: Description: Category: Unreleased Structures

2021-12-15T15:45:36Z

New page: '''Unreleased structure''' The entry 7t4e is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7t4e is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]