7tty - Revision history

OCA at 09:13, 17 October 2024

2024-10-17T09:13:19Z

← Older revision		Revision as of 09:13, 17 October 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tty OCA], [https://pdbe.org/7tty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tty RCSB], [https://www.ebi.ac.uk/pdbsum/7tty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tty ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tty OCA], [https://pdbe.org/7tty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tty RCSB], [https://www.ebi.ac.uk/pdbsum/7tty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tty ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	[https://www.uniprot.org/uniprot/U5WI05_SARS U5WI05_SARS] Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.[HAMAP-Rule:MF_04099] Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099] Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
			*[[Spike protein 3D structures\|Spike protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:07, 18 October 2023

2023-10-18T17:07:51Z

← Older revision		Revision as of 17:07, 18 October 2023
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	====		==Crystal structure of potent neutralizing antibody 10-40 in complex with bat WIV1 receptor-binding domain==
	<StructureSection load='7tty' size='340' side='right'caption='[[7tty]]' scene=''>		<StructureSection load='7tty' size='340' side='right'caption='[[7tty]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7tty]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bat_SARS-like_coronavirus_WIV1 Bat SARS-like coronavirus WIV1] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TTY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tty OCA], [https://pdbe.org/7tty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tty RCSB], [https://www.ebi.ac.uk/pdbsum/7tty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tty ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.11Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tty OCA], [https://pdbe.org/7tty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tty RCSB], [https://www.ebi.ac.uk/pdbsum/7tty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tty ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/U5WI05_SARS U5WI05_SARS] Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.[HAMAP-Rule:MF_04099] Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099] Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The devastation caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made clear the importance of pandemic preparedness. To address future zoonotic outbreaks due to related viruses in the sarbecovirus subgenus, we identified a human monoclonal antibody, 10-40, that neutralized or bound all sarbecoviruses tested in vitro and protected against SARS-CoV-2 and SARS-CoV in vivo. Comparative studies with other receptor-binding domain (RBD)-directed antibodies showed 10-40 to have the greatest breadth against sarbecoviruses, suggesting that 10-40 is a promising agent for pandemic preparedness. Moreover, structural analyses on 10-40 and similar antibodies not only defined an epitope cluster in the inner face of the RBD that is well conserved among sarbecoviruses but also uncovered a distinct antibody class with a common CDRH3 motif. Our analyses also suggested that elicitation of this class of antibodies may not be overly difficult, an observation that bodes well for the development of a pan-sarbecovirus vaccine.

			An antibody class with a common CDRH3 motif broadly neutralizes sarbecoviruses.,Liu L, Iketani S, Guo Y, Reddem ER, Casner RG, Nair MS, Yu J, Chan JF, Wang M, Cerutti G, Li Z, Morano NC, Castagna CD, Corredor L, Chu H, Yuan S, Poon VK, Chan CC, Chen Z, Luo Y, Cunningham M, Chavez A, Yin MT, Perlin DS, Tsuji M, Yuen KY, Kwong PD, Sheng Z, Huang Y, Shapiro L, Ho DD Sci Transl Med. 2022 May 25;14(646):eabn6859. doi: 10.1126/scitranslmed.abn6859. , Epub 2022 May 25. PMID:35438546<ref>PMID:35438546</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7tty" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Antibody 3D structures\|Antibody 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bat SARS-like coronavirus WIV1]]
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Reddem ER]]
			[[Category: Shapiro L]]

OCA at 12:53, 4 May 2022

2022-05-04T12:53:01Z

← Older revision		Revision as of 12:53, 4 May 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7tty ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7tty' size='340' side='right'caption='[[7tty]]' scene=''>
	~~Authors~~: ~~Reddem, E~~.R.~~, Shapiro, L~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Crystal structure of potent neutralizing antibody 10~~-~~40 in complex with bat WIV1 receptor-binding domain~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tty OCA], [https://pdbe.org/7tty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tty RCSB], [https://www.ebi.ac.uk/pdbsum/7tty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tty ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[Category: ~~Shapiro, L~~]]		__TOC__
	[[Category: ~~Reddem, E.R~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 09:09, 16 February 2022

2022-02-16T09:09:26Z

← Older revision		Revision as of 09:09, 16 February 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7tty is ON HOLD		The entry 7tty is ON HOLD until Paper Publication

	Authors: Reddem, E.R., Shapiro, L.		Authors: Reddem, E.R., Shapiro, L.

	Description: Crystal structure of potent neutralizing antibody 10-40 in complex with bat ~~WIVI~~ receptor-binding domain		Description: Crystal structure of potent neutralizing antibody 10-40 in complex with bat WIV1 receptor-binding domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Shapiro, L]]		[[Category: Shapiro, L]]
	[[Category: Reddem, E.R]]		[[Category: Reddem, E.R]]

OCA: Protected "7tty" [edit=sysop:move=sysop]

2022-02-09T06:21:50Z

Protected "7tty" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:21, 9 February 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7tty is ON HOLD Authors: Reddem, E.R., Shapiro, L. Description: Crystal structure of potent neutralizing antibody 10-40 in complex with bat WIVI re...

2022-02-09T06:21:49Z

New page: '''Unreleased structure''' The entry 7tty is ON HOLD Authors: Reddem, E.R., Shapiro, L. Description: Crystal structure of potent neutralizing antibody 10-40 in complex with bat WIVI re...

New page

'''Unreleased structure'''

The entry 7tty is ON HOLD

Authors: Reddem, E.R., Shapiro, L.

Description: Crystal structure of potent neutralizing antibody 10-40 in complex with bat WIVI receptor-binding domain
[[Category: Unreleased Structures]]
[[Category: Shapiro, L]]
[[Category: Reddem, E.R]]