7unc - Revision history

OCA at 11:37, 23 October 2024

2024-10-23T11:37:39Z

← Older revision		Revision as of 11:37, 23 October 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7unc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[7unc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UNC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7unc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7unc OCA], [https://pdbe.org/7unc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7unc RCSB], [https://www.ebi.ac.uk/pdbsum/7unc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7unc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7unc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7unc OCA], [https://pdbe.org/7unc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7unc RCSB], [https://www.ebi.ac.uk/pdbsum/7unc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7unc ProSAT]</span></td></tr>
	</table>		</table>
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	In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past nucleosomes, often resulting in nucleosome displacement. How Pol II unwraps the DNA from nucleosomes to allow transcription and how DNA rewraps to retain nucleosomes has been unclear. Here, we report the 3.0-angstrom cryo-electron microscopy structure of a mammalian Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex stalled 54 base pairs within the nucleosome. The structure provides a mechanistic basis for nucleosome retention during transcription elongation where upstream DNA emerging from the Pol II cleft has rewrapped the proximal side of the nucleosome. The structure uncovers a direct role for Pol II and transcription elongation factors in nucleosome retention and explains how nucleosomes are retained to prevent the disruption of chromatin structure across actively transcribed genes.		In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past nucleosomes, often resulting in nucleosome displacement. How Pol II unwraps the DNA from nucleosomes to allow transcription and how DNA rewraps to retain nucleosomes has been unclear. Here, we report the 3.0-angstrom cryo-electron microscopy structure of a mammalian Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex stalled 54 base pairs within the nucleosome. The structure provides a mechanistic basis for nucleosome retention during transcription elongation where upstream DNA emerging from the Pol II cleft has rewrapped the proximal side of the nucleosome. The structure uncovers a direct role for Pol II and transcription elongation factors in nucleosome retention and explains how nucleosomes are retained to prevent the disruption of chromatin structure across actively transcribed genes.

	Structural basis of nucleosome retention during transcription elongation.,Filipovski M, Soffers JHM, Vos SM, Farnung L Science. 2022 Jun 17;376(6599):1313-1316. doi: 10.1126/science.abo3851. Epub 2022, Jun 16. PMID:35709268<ref>PMID:35709268</ref>		Structural basis of nucleosome retention during transcription elongation.,Filipovski M, Soffers JHM, Vos SM, Farnung L Science. 2022 Jun 17;376(6599):1313-1316. doi: 10.1126/science.abo3851. Epub 2022 , Jun 16. PMID:35709268<ref>PMID:35709268</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	<div class="pdbe-citations 7unc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7unc" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:20, 19 October 2022

2022-10-19T19:20:32Z

@@ Line 1: / Line 1: @@
-The entry 7unc is ON HOLD
+==Pol II-DSIF-SPT6-PAF1c-TFIIS complex with rewrapped nucleosome==
-Authors:
+Structural basis of nucleosome retention during transcription elongation.,Filipovski M, Soffers JHM, Vos SM, Farnung L Science. 2022 Jun 17;376(6599):1313-1316. doi: 10.1126/science.abo3851. Epub 2022, Jun 16. PMID:35709268<ref>PMID:35709268</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>

OCA: Protected "7unc" [edit=sysop:move=sysop]

2022-04-21T01:35:33Z

Protected "7unc" [edit=sysop:move=sysop]

← Older revision	Revision as of 01:35, 21 April 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7unc is ON HOLD Authors: Description: Category: Unreleased Structures

2022-04-21T01:35:32Z

New page: '''Unreleased structure''' The entry 7unc is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7unc is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]