7usr - Revision history

OCA at 06:50, 21 November 2024

2024-11-21T06:50:52Z

← Older revision		Revision as of 06:50, 21 November 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/P230_PLAF7 P230_PLAF7] Gametocyte surface protein required for male/female gamete fusion. Also required for male gamete exflagellation and interaction with erythrocytes.<ref>PMID:16879650</ref>		[https://www.uniprot.org/uniprot/P230_PLAF7 P230_PLAF7] Gametocyte surface protein required for male/female gamete fusion. Also required for male gamete exflagellation and interaction with erythrocytes.<ref>PMID:16879650</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Transmission blocking interventions can stop malaria parasite transmission from mosquito to human by inhibiting parasite infection in mosquitos. One of the most advanced candidates for a malaria transmission blocking vaccine is Pfs230. Pfs230 is the largest member of the 6-cysteine protein family with 14 consecutive 6-cysteine domains and is expressed on the surface of gametocytes and gametes. Here, we present the crystal structure of the first two 6-cysteine domains of Pfs230. We identified high affinity Pfs230-specific nanobodies that recognized gametocytes and bind to distinct sites on Pfs230, which were isolated from immunized alpacas. Using two non-overlapping Pfs230 nanobodies, we show that these nanobodies significantly blocked P. falciparum transmission and reduced the formation of exflagellation centers. Crystal structures of the transmission blocking nanobodies with the first 6-cysteine domain of Pfs230 confirm that they bind to different epitopes. In addition, these nanobodies bind to Pfs230 in the absence of the prodomain, in contrast with the binding of known Pfs230 transmission blocking antibodies. These results provide additional structural insight into Pfs230 domains and elucidate a mechanism of action of transmission blocking Pfs230 nanobodies.

			Nanobodies against Pfs230 block Plasmodium falciparum transmission.,Dietrich MH, Gabriela M, Reaksudsan K, Dixon MWA, Chan LJ, Adair A, Trickey S, O'Neill MT, Tan LL, Lopaticki S, Healer J, Keremane S, Cowman AF, Tham WH Biochem J. 2022 Dec 22;479(24):2529-2546. doi: 10.1042/BCJ20220554. PMID:36520108<ref>PMID:36520108</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7usr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:31, 12 July 2023

2023-07-12T07:31:23Z

← Older revision		Revision as of 07:31, 12 July 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7usr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7USR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7USR FirstGlance]. <br>		<table><tr><td colspan='2'>[[7usr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7USR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7USR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7usr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7usr OCA], [https://pdbe.org/7usr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7usr RCSB], [https://www.ebi.ac.uk/pdbsum/7usr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7usr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7usr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7usr OCA], [https://pdbe.org/7usr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7usr RCSB], [https://www.ebi.ac.uk/pdbsum/7usr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7usr ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:52, 28 December 2022

2022-12-28T08:52:48Z

← Older revision		Revision as of 08:52, 28 December 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7usr is ~~ON HOLD until Paper Publication~~		==Plasmodium falciparum protein Pfs230 D1D2 - Structure of the first two 6-cysteine domains==
			<StructureSection load='7usr' size='340' side='right'caption='[[7usr]], [[Resolution\|resolution]] 1.93Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[7usr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7USR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7USR FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7usr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7usr OCA], [https://pdbe.org/7usr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7usr RCSB], [https://www.ebi.ac.uk/pdbsum/7usr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7usr ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/P230_PLAF7 P230_PLAF7] Gametocyte surface protein required for male/female gamete fusion. Also required for male gamete exflagellation and interaction with erythrocytes.<ref>PMID:16879650</ref>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Plasmodium falciparum 3D7]]
			[[Category: Dietrich MH]]
			[[Category: Tham WH]]

OCA at 06:41, 25 May 2022

2022-05-25T06:41:13Z

← Older revision		Revision as of 06:41, 25 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7usr is ON HOLD		The entry 7usr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7usr" [edit=sysop:move=sysop]

2022-05-04T11:33:20Z

Protected "7usr" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:33, 4 May 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7usr is ON HOLD Authors: Description: Category: Unreleased Structures

2022-05-04T11:33:19Z

New page: '''Unreleased structure''' The entry 7usr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7usr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]