7v21 - Revision history

OCA at 05:15, 12 June 2024

2024-06-12T05:15:15Z

← Older revision		Revision as of 05:15, 12 June 2024
Line 3:		Line 3:
	<StructureSection load='7v21' size='340' side='right'caption='[[7v21]], [[Resolution\|resolution]] 3.08Å' scene=''>		<StructureSection load='7v21' size='340' side='right'caption='[[7v21]], [[Resolution\|resolution]] 3.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7v21]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V21 FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V21 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v21 OCA], [https://pdbe.org/7v21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v21 RCSB], [https://www.ebi.ac.uk/pdbsum/7v21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v21 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.08Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v21 OCA], [https://pdbe.org/7v21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v21 RCSB], [https://www.ebi.ac.uk/pdbsum/7v21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v21 ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	[https://www.uniprot.org/uniprot/LACTB_HUMAN LACTB_HUMAN] Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (PubMed:28329758). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (PubMed:28329758). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (PubMed:28329758). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of breast cancer cells: probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed:28329758).<ref>PMID:28329758</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Serine beta-lactamase-like protein (LACTB) is a mammalian mitochondrial serine protease that can specifically hydrolyze peptide bonds adjacent to aspartic acid residues and is structurally related to prokaryotic penicillin-binding proteins. Here, we determined the cryoelectron microscopy structures of human LACTB (hLACTB) filaments from wild-type protein, a middle region deletion mutant, and in complex with the inhibitor Z-AAD-CMK at 3.0-, 3.1-, and 2.8-A resolution, respectively. Structural analysis and activity assays revealed that three interfaces are required for the assembly of hLACTB filaments and that the formation of higher order helical structures facilitates its cleavage activity. Further structural and enzymatic analyses of middle region deletion constructs indicated that, while this region is necessary for substrate hydrolysis, it is not required for filament formation. Moreover, the inhibitor-bound structure showed that hLACTB may cleave peptide bonds adjacent to aspartic acid residues. These findings provide the structural basis underlying hLACTB catalytic activity.

	Structural basis for the catalytic activity of filamentous human serine beta-lactamase-like protein LACTB.,Zhang M, Zhang L, Guo R, Xiao C, Yin J, Zhang S, Yang M Structure. 2022 May 5;30(5):685-696.e5. doi: 10.1016/j.str.2022.02.007. Epub 2022 , Mar 4. PMID:35247327<ref>PMID:35247327</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7v21" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Homo sapiens]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Yang MJ]]		[[Category: Yang MJ]]
	[[Category: Zhang MH]]		[[Category: Zhang MH]]

OCA at 06:12, 2 March 2023

2023-03-02T06:12:23Z

← Older revision		Revision as of 06:12, 2 March 2023
Line 1:		Line 1:

	==human Serine beta-lactamase-like protein LACTB truncation variant==		==human Serine beta-lactamase-like protein LACTB truncation variant==
	<StructureSection load='7v21' size='340' side='right'caption='[[7v21]]' scene=''>		<StructureSection load='7v21' size='340' side='right'caption='[[7v21]], [[Resolution\|resolution]] 3.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V21 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7v21]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V21 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v21 OCA], [https://pdbe.org/7v21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v21 RCSB], [https://www.ebi.ac.uk/pdbsum/7v21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v21 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v21 OCA], [https://pdbe.org/7v21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v21 RCSB], [https://www.ebi.ac.uk/pdbsum/7v21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v21 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/LACTB_HUMAN LACTB_HUMAN] Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (PubMed:28329758). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (PubMed:28329758). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (PubMed:28329758). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of breast cancer cells: probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed:28329758).<ref>PMID:28329758</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Serine beta-lactamase-like protein (LACTB) is a mammalian mitochondrial serine protease that can specifically hydrolyze peptide bonds adjacent to aspartic acid residues and is structurally related to prokaryotic penicillin-binding proteins. Here, we determined the cryoelectron microscopy structures of human LACTB (hLACTB) filaments from wild-type protein, a middle region deletion mutant, and in complex with the inhibitor Z-AAD-CMK at 3.0-, 3.1-, and 2.8-A resolution, respectively. Structural analysis and activity assays revealed that three interfaces are required for the assembly of hLACTB filaments and that the formation of higher order helical structures facilitates its cleavage activity. Further structural and enzymatic analyses of middle region deletion constructs indicated that, while this region is necessary for substrate hydrolysis, it is not required for filament formation. Moreover, the inhibitor-bound structure showed that hLACTB may cleave peptide bonds adjacent to aspartic acid residues. These findings provide the structural basis underlying hLACTB catalytic activity.

			Structural basis for the catalytic activity of filamentous human serine beta-lactamase-like protein LACTB.,Zhang M, Zhang L, Guo R, Xiao C, Yin J, Zhang S, Yang M Structure. 2022 May 5;30(5):685-696.e5. doi: 10.1016/j.str.2022.02.007. Epub 2022 , Mar 4. PMID:35247327<ref>PMID:35247327</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7v21" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Yang MJ]]		[[Category: Yang MJ]]
	[[Category: Zhang MH]]		[[Category: Zhang MH]]

OCA at 10:19, 16 February 2022

2022-02-16T10:19:16Z

← Older revision		Revision as of 10:19, 16 February 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7v21 is ~~ON HOLD~~		==human Serine beta-lactamase-like protein LACTB truncation variant==
			<StructureSection load='7v21' size='340' side='right'caption='[[7v21]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V21 FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v21 OCA], [https://pdbe.org/7v21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v21 RCSB], [https://www.ebi.ac.uk/pdbsum/7v21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v21 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Yang MJ]]
			[[Category: Zhang MH]]

OCA at 08:41, 12 January 2022

2022-01-12T08:41:07Z

← Older revision		Revision as of 08:41, 12 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7v21 is ON HOLD ~~until Paper Publication~~		The entry 7v21 is ON HOLD

	Authors: ~~Zhang, M.H.~~		Authors:

	Description: ~~Mitochondrial intermembrane space protease truncation~~		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	~~[[Category: Zhang, M.H]]~~

OCA at 07:51, 29 September 2021

2021-09-29T07:51:30Z

← Older revision		Revision as of 07:51, 29 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7v21 is ON HOLD		The entry 7v21 is ON HOLD until Paper Publication

	Authors: Zhang, M.H.		Authors: Zhang, M.H.

OCA: Protected "7v21" [edit=sysop:move=sysop]

2021-08-18T05:13:03Z

Protected "7v21" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:13, 18 August 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7v21 is ON HOLD Authors: Zhang, M.H. Description: Mitochondrial intermembrane space protease truncation Category: Unreleased Structures [[Categ...

2021-08-18T05:13:02Z

New page: '''Unreleased structure''' The entry 7v21 is ON HOLD Authors: Zhang, M.H. Description: Mitochondrial intermembrane space protease truncation Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 7v21 is ON HOLD

Authors: Zhang, M.H.

Description: Mitochondrial intermembrane space protease truncation
[[Category: Unreleased Structures]]
[[Category: Zhang, M.H]]