7vb2 - Revision history

OCA at 11:21, 14 June 2023

2023-06-14T11:21:36Z

← Older revision		Revision as of 11:21, 14 June 2023
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	==Solution structure of human ribosomal protein uL11==		==Solution structure of human ribosomal protein uL11==
	<StructureSection load='7vb2' size='340' side='right'caption='[[7vb2~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>		<StructureSection load='7vb2' size='340' side='right'caption='[[7vb2]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7vb2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VB2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7vb2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VB2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vb2 OCA], [https://pdbe.org/7vb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vb2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vb2 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vb2 OCA], [https://pdbe.org/7vb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vb2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vb2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RL12_HUMAN RL12_HUMAN]] Binds directly to 26S ribosomal RNA (By similarity).		[https://www.uniprot.org/uniprot/RL12_HUMAN RL12_HUMAN] Binds directly to 26S ribosomal RNA (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lee~~, K M~~]]		[[Category: Lee KM]]
	[[Category: Wong~~, K B]]~~		[[Category: Wong KB]]
	~~[[Category: Elongation factor]]~~
	~~[[Category: Ribosomal protein]]~~
	~~[[Category: Translation~~]]

OCA at 08:18, 25 May 2022

2022-05-25T08:18:36Z

← Older revision		Revision as of 08:18, 25 May 2022
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	==Solution structure of human ribosomal protein uL11==		==Solution structure of human ribosomal protein uL11==
	<StructureSection load='7vb2' size='340' side='right'caption='[[7vb2]]' scene=''>		<StructureSection load='7vb2' size='340' side='right'caption='[[7vb2]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VB2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7vb2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VB2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vb2 OCA], [https://pdbe.org/7vb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vb2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vb2 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vb2 OCA], [https://pdbe.org/7vb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vb2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vb2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/RL12_HUMAN RL12_HUMAN]] Binds directly to 26S ribosomal RNA (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Eukaryotic uL11 contains a conserved MPPKFDP motif at the N-terminus that is not found in archaeal and bacterial homologs. Here, we determined the solution structure of human uL11 by NMR spectroscopy and characterized its backbone dynamics by 15N-1H relaxation experiments. We showed that these N-terminal residues are unstructured and flexible. Structural comparison with ribosome-bound uL11 suggests that the linker region between the N-terminal domain and C-terminal domain of human uL11 is intrinsically disordered and only becomes structured when bound to the ribosomes. Mutagenesis studies show that the N-terminal conserved MPPKFDP motif is involved in interacting with the P-complex and its extended protuberant domain of uL10 in vitro. Truncation of the MPPKFDP motif also reduced the poly-phenylalanine synthesis in both hybrid ribosome and yeast mutagenesis studies. In addition, G-->A/P substitutions to the conserved GPLG motif of helix-1 reduced poly-phenylalanine synthesis to 9-32% in yeast ribosomes. We propose that the flexible N-terminal residues of uL11, which could extend up to approximately 25 A from the N-terminal domain of uL11, can form transient interactions with the uL10 that help to fetch and fix it into a position ready for recruiting the incoming translation factors and facilitate protein synthesis.

			The flexible N-terminal motif of uL11 unique to eukaryotic ribosomes interacts with P-complex and facilitates protein translation.,Yang L, Lee KM, Yu CW, Imai H, Choi AK, Banfield DK, Ito K, Uchiumi T, Wong KB Nucleic Acids Res. 2022 May 20;50(9):5335-5348. doi: 10.1093/nar/gkac292. PMID:35544198<ref>PMID:35544198</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7vb2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lee KM]]		[[Category: Lee, K M]]
	[[Category: Wong KB]]		[[Category: Wong, K B]]
			[[Category: Elongation factor]]
			[[Category: Ribosomal protein]]
			[[Category: Translation]]

OCA at 02:57, 21 April 2022

2022-04-21T02:57:53Z

← Older revision		Revision as of 02:57, 21 April 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7vb2 is ~~ON HOLD until Paper Publication~~		==Solution structure of human ribosomal protein uL11==
			<StructureSection load='7vb2' size='340' side='right'caption='[[7vb2]]' scene=''>
	~~Authors~~: ~~Lee~~, K.M., ~~Wong~~, K.B.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VB2 FirstGlance]. <br>
	~~Description: Solution structure of human ribosomal protein uL11~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vb2 OCA], [https://pdbe.org/7vb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vb2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vb2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Lee~~, K.M~~]]		__TOC__
	[[Category: Wong~~, K.B~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Lee KM]]
			[[Category: Wong KB]]

OCA at 12:17, 29 December 2021

2021-12-29T12:17:37Z

← Older revision		Revision as of 12:17, 29 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7vb2 is ON HOLD		The entry 7vb2 is ON HOLD until Paper Publication

	Authors: Lee, K.M., Wong, K.B.		Authors: Lee, K.M., Wong, K.B.

OCA: Protected "7vb2" [edit=sysop:move=sysop]

2021-09-08T08:02:17Z

Protected "7vb2" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:02, 8 September 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7vb2 is ON HOLD Authors: Lee, K.M., Wong, K.B. Description: Solution structure of human ribosomal protein uL11 Category: Unreleased Structures ...

2021-09-08T08:02:16Z

New page: '''Unreleased structure''' The entry 7vb2 is ON HOLD Authors: Lee, K.M., Wong, K.B. Description: Solution structure of human ribosomal protein uL11 Category: Unreleased Structures ...

New page

'''Unreleased structure'''

The entry 7vb2 is ON HOLD

Authors: Lee, K.M., Wong, K.B.

Description: Solution structure of human ribosomal protein uL11
[[Category: Unreleased Structures]]
[[Category: Lee, K.M]]
[[Category: Wong, K.B]]