7x77 - Revision history

OCA at 09:41, 9 October 2024

2024-10-09T09:41:40Z

← Older revision		Revision as of 09:41, 9 October 2024
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	<StructureSection load='7x77' size='340' side='right'caption='[[7x77]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='7x77' size='340' side='right'caption='[[7x77]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7x77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7x77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Autographa_californica_multiple_nucleopolyhedrovirus Autographa californica multiple nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X77 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x77 OCA], [https://pdbe.org/7x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x77 RCSB], [https://www.ebi.ac.uk/pdbsum/7x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x77 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x77 OCA], [https://pdbe.org/7x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x77 RCSB], [https://www.ebi.ac.uk/pdbsum/7x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x77 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PIF5_NPVAC PIF5_NPVAC] Structural protein that plays a role in the first steps of per os larva infection.<ref>PMID:20970820</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Baculoviruses initiate oral infection in the highly alkaline midgut of insects via a group of envelope proteins called per os infectivity factors (PIFs). To date, no high-resolution structural information has been reported for any PIF. Here, we present the crystal structure of the PIF5 ectodomain (PIF5e) from Autographa californica multiple nucleopolyhedrovirus (AcMNPV) at a 2.2-A resolution. It revealed an open cavity between the N-terminal E1 domain and the C-terminal E2 domain and a cysteine-rich region with three pairs of disulfide bonds in the E2 domain. Multiple conserved intramolecular interactions within PIF5 are essential for maintaining its tertiary structure. Two conserved arginines (Arg8 and Arg74) play critical roles in E1-E2 interactions, and mutagenesis analysis supported their crucial role in oral infection. Importantly, the reduction in the oral infectivity of the Arg8, Arg74, or cysteine mutant viruses was related to the proteolytic cleavage of PIF5 by the endogenous protease embedded in occlusion bodies during alkaline treatment. This suggested that the structural stability of PIF5 under physiological conditions in the insect midgut is critical for baculoviral oral infectivity. ~~IMPORTANCE Per~~ os infection mediated by PIFs is the highly complex mechanism by which baculoviruses initiate infection in insects. Previous studies revealed that multiple PIF proteins form a large PIF complex on the envelope of virions, while PIF5 functions independently of the PIF complex. Here, we report the crystal structure of AcMNPV PIF5e, which, to our knowledge, is the first atomic structure reported for a PIF protein. The structure revealed the precise locations of three previously proposed disulfide bonds and other conserved intramolecular interactions, which are important for the structural stability of PIF5 and are also essential for oral infectivity. These findings advance our understanding of the molecular mechanism of baculovirus oral infection under alkaline conditions.		Baculoviruses initiate oral infection in the highly alkaline midgut of insects via a group of envelope proteins called per os infectivity factors (PIFs). To date, no high-resolution structural information has been reported for any PIF. Here, we present the crystal structure of the PIF5 ectodomain (PIF5e) from Autographa californica multiple nucleopolyhedrovirus (AcMNPV) at a 2.2-A resolution. It revealed an open cavity between the N-terminal E1 domain and the C-terminal E2 domain and a cysteine-rich region with three pairs of disulfide bonds in the E2 domain. Multiple conserved intramolecular interactions within PIF5 are essential for maintaining its tertiary structure. Two conserved arginines (Arg8 and Arg74) play critical roles in E1-E2 interactions, and mutagenesis analysis supported their crucial role in oral infection. Importantly, the reduction in the oral infectivity of the Arg8, Arg74, or cysteine mutant viruses was related to the proteolytic cleavage of PIF5 by the endogenous protease embedded in occlusion bodies during alkaline treatment. This suggested that the structural stability of PIF5 under physiological conditions in the insect midgut is critical for baculoviral oral infectivity. IMPORTANCEPer os infection mediated by PIFs is the highly complex mechanism by which baculoviruses initiate infection in insects. Previous studies revealed that multiple PIF proteins form a large PIF complex on the envelope of virions, while PIF5 functions independently of the PIF complex. Here, we report the crystal structure of AcMNPV PIF5e, which, to our knowledge, is the first atomic structure reported for a PIF protein. The structure revealed the precise locations of three previously proposed disulfide bonds and other conserved intramolecular interactions, which are important for the structural stability of PIF5 and are also essential for oral infectivity. These findings advance our understanding of the molecular mechanism of baculovirus oral infection under alkaline conditions.

	Structural Characterization of Per Os Infectivity Factor 5 (PIF5) Reveals the Essential Role of Intramolecular Interactions in Baculoviral Oral Infectivity.,Li Z, Zhang H, Li Z, Fu Y, Wang X, Li J, Wang K, Wang Z, Zhang T, Wang M, Hu Z, Cao S J Virol. 2022 Jul 27;96(14):e0080622. doi: 10.1128/jvi.00806-22. Epub 2022 Jul 7. PMID:35862697<ref>PMID:35862697</ref>		Structural Characterization of Per Os Infectivity Factor 5 (PIF5) Reveals the Essential Role of Intramolecular Interactions in Baculoviral Oral Infectivity.,Li Z, Zhang H, Li Z, Fu Y, Wang X, Li J, Wang K, Wang Z, Zhang T, Wang M, Hu Z, Cao S J Virol. 2022 Jul 27;96(14):e0080622. doi: 10.1128/jvi.00806-22. Epub 2022 Jul 7. PMID:35862697<ref>PMID:35862697</ref>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Autographa californica multiple nucleopolyhedrovirus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cao, S]]		[[Category: Cao S]]
	[[Category: Fu, Y]]		[[Category: Fu Y]]
	[[Category: Li, Z]]		[[Category: Li Z]]
	~~[[Category: Baculovirus]]~~
	~~[[Category: Cell entry]]~~
	~~[[Category: Membrane protein]]~~
	~~[[Category: Per os infectivity factor~~]]

OCA at 05:14, 10 August 2022

2022-08-10T05:14:47Z

← Older revision		Revision as of 05:14, 10 August 2022
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	==Ectodomain structure of per os infectivity factor 5==		==Ectodomain structure of per os infectivity factor 5==
	<StructureSection load='7x77' size='340' side='right'caption='[[7x77]]' scene=''>		<StructureSection load='7x77' size='340' side='right'caption='[[7x77]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7x77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X77 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x77 OCA], [https://pdbe.org/7x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x77 RCSB], [https://www.ebi.ac.uk/pdbsum/7x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x77 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x77 OCA], [https://pdbe.org/7x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x77 RCSB], [https://www.ebi.ac.uk/pdbsum/7x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x77 ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Baculoviruses initiate oral infection in the highly alkaline midgut of insects via a group of envelope proteins called per os infectivity factors (PIFs). To date, no high-resolution structural information has been reported for any PIF. Here, we present the crystal structure of the PIF5 ectodomain (PIF5e) from Autographa californica multiple nucleopolyhedrovirus (AcMNPV) at a 2.2-A resolution. It revealed an open cavity between the N-terminal E1 domain and the C-terminal E2 domain and a cysteine-rich region with three pairs of disulfide bonds in the E2 domain. Multiple conserved intramolecular interactions within PIF5 are essential for maintaining its tertiary structure. Two conserved arginines (Arg8 and Arg74) play critical roles in E1-E2 interactions, and mutagenesis analysis supported their crucial role in oral infection. Importantly, the reduction in the oral infectivity of the Arg8, Arg74, or cysteine mutant viruses was related to the proteolytic cleavage of PIF5 by the endogenous protease embedded in occlusion bodies during alkaline treatment. This suggested that the structural stability of PIF5 under physiological conditions in the insect midgut is critical for baculoviral oral infectivity. IMPORTANCE Per os infection mediated by PIFs is the highly complex mechanism by which baculoviruses initiate infection in insects. Previous studies revealed that multiple PIF proteins form a large PIF complex on the envelope of virions, while PIF5 functions independently of the PIF complex. Here, we report the crystal structure of AcMNPV PIF5e, which, to our knowledge, is the first atomic structure reported for a PIF protein. The structure revealed the precise locations of three previously proposed disulfide bonds and other conserved intramolecular interactions, which are important for the structural stability of PIF5 and are also essential for oral infectivity. These findings advance our understanding of the molecular mechanism of baculovirus oral infection under alkaline conditions.

			Structural Characterization of Per Os Infectivity Factor 5 (PIF5) Reveals the Essential Role of Intramolecular Interactions in Baculoviral Oral Infectivity.,Li Z, Zhang H, Li Z, Fu Y, Wang X, Li J, Wang K, Wang Z, Zhang T, Wang M, Hu Z, Cao S J Virol. 2022 Jul 27;96(14):e0080622. doi: 10.1128/jvi.00806-22. Epub 2022 Jul 7. PMID:35862697<ref>PMID:35862697</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7x77" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cao S]]		[[Category: Cao, S]]
	[[Category: Fu Y]]		[[Category: Fu, Y]]
	[[Category: Li Z]]		[[Category: Li, Z]]
			[[Category: Baculovirus]]
			[[Category: Cell entry]]
			[[Category: Membrane protein]]
			[[Category: Per os infectivity factor]]

OCA at 09:47, 22 June 2022

2022-06-22T09:47:44Z

← Older revision		Revision as of 09:47, 22 June 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7x77 is ~~ON HOLD until Paper Publication~~		==Ectodomain structure of per os infectivity factor 5==
			<StructureSection load='7x77' size='340' side='right'caption='[[7x77]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X77 FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x77 OCA], [https://pdbe.org/7x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x77 RCSB], [https://www.ebi.ac.uk/pdbsum/7x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x77 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Cao S]]
			[[Category: Fu Y]]
			[[Category: Li Z]]

OCA at 09:59, 23 March 2022

2022-03-23T09:59:30Z

← Older revision		Revision as of 09:59, 23 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7x77 is ON HOLD		The entry 7x77 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7x77" [edit=sysop:move=sysop]

2022-03-16T05:13:43Z

Protected "7x77" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:13, 16 March 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7x77 is ON HOLD Authors: Description: Category: Unreleased Structures

2022-03-16T05:13:42Z

New page: '''Unreleased structure''' The entry 7x77 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7x77 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]