7ync - Revision history

OCA at 07:33, 3 July 2024

2024-07-03T07:33:42Z

← Older revision		Revision as of 07:33, 3 July 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ync]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ync]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YNC FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ync OCA], [https://pdbe.org/7ync PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ync RCSB], [https://www.ebi.ac.uk/pdbsum/7ync PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ync ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.14Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ync OCA], [https://pdbe.org/7ync PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ync RCSB], [https://www.ebi.ac.uk/pdbsum/7ync PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ync ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~A0A401FT36_9DELT A0A401FT36_9DELT~~]		[https://www.uniprot.org/uniprot/A0A401FT36_9BACT A0A401FT36_9BACT]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The recently discovered type III-E CRISPR-Cas effector Cas7-11 shows promise when used as an RNA manipulation tool, but its structure and the mechanisms underlying its function remain unclear. Here we present four cryo-EM structures of Desulfonema ishimotonii Cas7-11-crRNA complex in pre-target and target RNA-bound states, and the cryo-EM structure of DiCas7-11-crRNA bound to its accessory protein DiCsx29. These data reveal structural elements for pre-crRNA processing, target RNA cleavage and regulation. Moreover, a 3' seed region of crRNA is involved in regulating RNA cleavage activity of DiCas7-11-crRNA-Csx29. Our analysis also shows that both the minimal mismatch of 4 nt to the 5' handle of crRNA and the minimal matching of the first 12 nt of the spacer by the target RNA are essential for triggering the protease activity of DiCas7-11-crRNA-Csx29 towards DiCsx30. Taken together, we propose that target RNA recognition and cleavage regulate and fine-tune the protease activity of DiCas7-11-crRNA-Csx29, thus preventing auto-immune responses.

			Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector.,Huo Y, Zhao H, Dong Q, Jiang T Nat Microbiol. 2023 Mar;8(3):522-532. doi: 10.1038/s41564-022-01316-4. Epub 2023 , Jan 26. PMID:36702942<ref>PMID:36702942</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ync" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:18, 1 February 2023

2023-02-01T11:18:27Z

← Older revision		Revision as of 11:18, 1 February 2023
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	~~'''Unreleased structure'''~~

	~~The entry 7ync is ON HOLD until Paper Publication~~		==Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3==
			<StructureSection load='7ync' size='340' side='right'caption='[[7ync]], [[Resolution\|resolution]] 3.14Å' scene=''>
	~~Authors: Huo, Y., Dong, Q., Zhao, H., Jiang, T.~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[7ync]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YNC FirstGlance]. <br>
	~~Description:~~ Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ync OCA], [https://pdbe.org/7ync PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ync RCSB], [https://www.ebi.ac.uk/pdbsum/7ync PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ync ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[Category: ~~Jiang, T~~]]		== Function ==
	[[Category: ~~Zhao, H~~]]		[https://www.uniprot.org/uniprot/A0A401FT36_9DELT A0A401FT36_9DELT]
	[[Category: Dong, Q]]		__TOC__
	[[Category: Huo, Y]]		</StructureSection>
			[[Category: Desulfonema ishimotonii]]
			[[Category: Large Structures]]
			[[Category: Dong Q]]
			[[Category: Huo Y]]
			[[Category: Jiang T]]
			[[Category: Zhao H]]

OCA at 03:34, 25 August 2022

2022-08-25T03:34:39Z

← Older revision		Revision as of 03:34, 25 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7ync is ON HOLD		The entry 7ync is ON HOLD until Paper Publication

	Authors: ~~Yangao~~, H., ~~Qinghua~~, D., ~~Hongshen~~, Z., ~~Tao~~, J.		Authors: Huo, Y., Dong, Q., Zhao, H., Jiang, T.

	Description: Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3		Description: Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: ~~Qinghua~~, D]]		[[Category: Jiang, T]]
	[[Category: ~~Tao~~, J]]		[[Category: Zhao, H]]
	[[Category: ~~Hongshen~~, Z]]		[[Category: Dong, Q]]
	[[Category: ~~Yangao~~, H]]		[[Category: Huo, Y]]

OCA: Protected "7ync" [edit=sysop:move=sysop]

2022-08-10T03:45:32Z

Protected "7ync" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:45, 10 August 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ync is ON HOLD Authors: Yangao, H., Qinghua, D., Hongshen, Z., Tao, J. Description: Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3 [[Cat...

2022-08-10T03:45:31Z

New page: '''Unreleased structure''' The entry 7ync is ON HOLD Authors: Yangao, H., Qinghua, D., Hongshen, Z., Tao, J. Description: Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3 [[Cat...

New page

'''Unreleased structure'''

The entry 7ync is ON HOLD

Authors: Yangao, H., Qinghua, D., Hongshen, Z., Tao, J.

Description: Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3
[[Category: Unreleased Structures]]
[[Category: Qinghua, D]]
[[Category: Tao, J]]
[[Category: Hongshen, Z]]
[[Category: Yangao, H]]