7zos - Revision history

OCA at 08:05, 7 February 2024

2024-02-07T08:05:33Z

← Older revision		Revision as of 08:05, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7zos]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beta_vulgaris Beta vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZOS FirstGlance]. <br>		<table><tr><td colspan='2'>[[7zos]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beta_vulgaris Beta vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZOS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=FC6:HEXACYANOFERRATE(3-)'>FC6</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=FC6:HEXACYANOFERRATE(3-)'>FC6</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zos OCA], [https://pdbe.org/7zos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zos RCSB], [https://www.ebi.ac.uk/pdbsum/7zos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zos ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zos OCA], [https://pdbe.org/7zos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zos RCSB], [https://www.ebi.ac.uk/pdbsum/7zos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zos ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV]		[https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Phytoglobins (Pgbs) are plant-originating heme proteins of the globin superfamily with varying degrees of hexacoordination. Pgbs have a conserved cysteine residue, the role of which is poorly understood. In this paper, we investigated the functional and structural role of cysteine in BvPgb1.2, a Class 1 Pgb from sugar beet (Beta vulgaris), by constructing an alanine-substituted mutant (Cys86Ala). The substitution had little impact on structure, dimerization, and heme loss as determined by X-ray crystallography, size-exclusion chromatography, and an apomyoglobin-based heme-loss assay, respectively. The substitution significantly affected other important biochemical properties. The autoxidation rate increased 16.7- and 14.4-fold for the mutant versus the native protein at 25 degrees C and 37 degrees C, respectively. Thermal stability similarly increased for the mutant by ~2.5 degrees C as measured by nano-differential scanning fluorimetry. Monitoring peroxidase activity over 7 days showed a 60% activity decrease in the native protein, from 33.7 to 20.2 U/mg protein. When comparing the two proteins, the mutant displayed a remarkable enzymatic stability as activity remained relatively constant throughout, albeit at a lower level, ~12 U/mg protein. This suggests that cysteine plays an important role in BvPgb1.2 function and stability, despite having seemingly little effect on its tertiary and quaternary structure.

	Oxidative Implications of Substituting a Conserved Cysteine Residue in Sugar Beet Phytoglobin BvPgb 1.2.,Christensen S, Groth L, Leiva-Eriksson N, Nyblom M, Bulow L Antioxidants (Basel). 2022 Aug 20;11(8). pii: antiox11081615. doi:, 10.3390/antiox11081615. PMID:36009334<ref>PMID:36009334</ref>		==See Also==
			*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]
	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7zos" style="background-color:#fffaf0;"></div>~~
	== ~~References~~ ==
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:14, 2 March 2023

2023-03-02T06:14:00Z

← Older revision		Revision as of 06:14, 2 March 2023
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	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV]]		[https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:46, 7 September 2022

2022-09-07T18:46:29Z

@@ Line 9: / Line 9: @@
 == Function ==
 [[https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV]]
 __TOC__
 </StructureSection>

OCA at 16:41, 7 September 2022

2022-09-07T16:41:11Z

← Older revision		Revision as of 16:41, 7 September 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7zos is ~~ON HOLD until Paper Publication~~		==Class 1 Phytoglobin from Sugar beet (BvPgb1.2)==
			<StructureSection load='7zos' size='340' side='right'caption='[[7zos]], [[Resolution\|resolution]] 1.90Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[7zos]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beta_vulgaris Beta vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZOS FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=FC6:HEXACYANOFERRATE(3-)'>FC6</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zos OCA], [https://pdbe.org/7zos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zos RCSB], [https://www.ebi.ac.uk/pdbsum/7zos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zos ProSAT]</span></td></tr>
			</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV]]
			__TOC__
			</StructureSection>
			[[Category: Beta vulgaris]]
			[[Category: Large Structures]]
			[[Category: Bulow L]]
			[[Category: Christensen S]]
			[[Category: Eriksson N]]
			[[Category: Nyblom M]]

OCA at 04:11, 8 June 2022

2022-06-08T04:11:27Z

← Older revision		Revision as of 04:11, 8 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7zos is ON HOLD		The entry 7zos is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7zos" [edit=sysop:move=sysop]

2022-05-04T11:48:52Z

Protected "7zos" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:48, 4 May 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7zos is ON HOLD Authors: Description: Category: Unreleased Structures

2022-05-04T11:48:51Z

New page: '''Unreleased structure''' The entry 7zos is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7zos is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]