8a0d - Revision history

OCA at 11:51, 23 October 2024

2024-10-23T11:51:30Z

← Older revision		Revision as of 11:51, 23 October 2024
Line 13:		Line 13:
	Allergens from furry animals frequently cause sensitization and respiratory allergic diseases. Most relevant mammalian respiratory allergens belong either to the protein family of lipocalins or secretoglobins. Their mechanism of sensitization remains largely unresolved. Mammalian lipocalin and secretoglobin allergens are associated with a function in chemical communication that involves abundant secretion into the environment, high stability and the ability to transport small volatile compounds. These properties are likely to contribute concomitantly to their allergenic potential. In this study, we aim to further elucidate the physiological function of lipocalin and secretoglobin allergens and link it to their sensitizing capacity, by analyzing their ligand-binding characteristics. We produced eight major mammalian respiratory allergens from four pet species in E.coli and compared their ligand-binding affinities to forty-nine ligands of different chemical classes by using a fluorescence-quenching assay. Furthermore, we solved the crystal-structure of the major guinea pig allergen Cav p 1, a typical lipocalin. Recombinant lipocalin and secretoglobin allergens are of high thermal stability with melting temperatures ranging from 65 to 90 degrees C and strongly bind ligands with dissociation constants in the low micromolar range, particularly fatty acids, fatty alcohols and the terpene alcohol farnesol, that are associated with potential semiochemical and/or immune-modulating functions. Through the systematic screening of respiratory mammalian lipocalin and secretoglobin allergens with a large panel of potential ligands, we observed that total amino acid composition, as well as cavity shape and volume direct affinities to ligands of different chemical classes. Therefore, we were able to categorize lipocalin allergens over their ligand-binding profile into three sub-groups of a lipocalin clade that is associated with functions in chemical communication, thus strengthening the function of major mammalian respiratory allergens as semiochemical carriers. The promiscuous binding capability of hydrophobic ligands from environmental sources warrants further investigation regarding their impact on a molecule's allergenicity.		Allergens from furry animals frequently cause sensitization and respiratory allergic diseases. Most relevant mammalian respiratory allergens belong either to the protein family of lipocalins or secretoglobins. Their mechanism of sensitization remains largely unresolved. Mammalian lipocalin and secretoglobin allergens are associated with a function in chemical communication that involves abundant secretion into the environment, high stability and the ability to transport small volatile compounds. These properties are likely to contribute concomitantly to their allergenic potential. In this study, we aim to further elucidate the physiological function of lipocalin and secretoglobin allergens and link it to their sensitizing capacity, by analyzing their ligand-binding characteristics. We produced eight major mammalian respiratory allergens from four pet species in E.coli and compared their ligand-binding affinities to forty-nine ligands of different chemical classes by using a fluorescence-quenching assay. Furthermore, we solved the crystal-structure of the major guinea pig allergen Cav p 1, a typical lipocalin. Recombinant lipocalin and secretoglobin allergens are of high thermal stability with melting temperatures ranging from 65 to 90 degrees C and strongly bind ligands with dissociation constants in the low micromolar range, particularly fatty acids, fatty alcohols and the terpene alcohol farnesol, that are associated with potential semiochemical and/or immune-modulating functions. Through the systematic screening of respiratory mammalian lipocalin and secretoglobin allergens with a large panel of potential ligands, we observed that total amino acid composition, as well as cavity shape and volume direct affinities to ligands of different chemical classes. Therefore, we were able to categorize lipocalin allergens over their ligand-binding profile into three sub-groups of a lipocalin clade that is associated with functions in chemical communication, thus strengthening the function of major mammalian respiratory allergens as semiochemical carriers. The promiscuous binding capability of hydrophobic ligands from environmental sources warrants further investigation regarding their impact on a molecule's allergenicity.

	Mammalian derived lipocalin and secretoglobin respiratory allergens strongly bind ligands with potentially immune modulating properties.,Janssen-Weets B, Kerff F, Swiontek K, Kler S, Czolk R, Revets D, Kuehn A, Bindslev-Jensen C, Ollert M, Hilger C Front Allergy. 2022 Aug 4;3:958711. doi: 10.3389/falgy.2022.958711. eCollection, 2022. PMID:35991307<ref>PMID:35991307</ref>		Mammalian derived lipocalin and secretoglobin respiratory allergens strongly bind ligands with potentially immune modulating properties.,Janssen-Weets B, Kerff F, Swiontek K, Kler S, Czolk R, Revets D, Kuehn A, Bindslev-Jensen C, Ollert M, Hilger C Front Allergy. 2022 Aug 4;3:958711. doi: 10.3389/falgy.2022.958711. eCollection , 2022. PMID:35991307<ref>PMID:35991307</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 13:36, 1 February 2024

2024-02-01T13:36:01Z

← Older revision		Revision as of 13:36, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a0d]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0D FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a0d]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0D FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.685Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A0A484HM70_CAVPO A0A484HM70_CAVPO]]		[https://www.uniprot.org/uniprot/A0A484HM70_CAVPO A0A484HM70_CAVPO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:54, 7 September 2022

2022-09-07T19:54:42Z

← Older revision		Revision as of 19:54, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='8a0d' size='340' side='right'caption='[[8a0d]], [[Resolution\|resolution]] 3.69Å' scene=''>		<StructureSection load='8a0d' size='340' side='right'caption='[[8a0d]], [[Resolution\|resolution]] 3.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a0d]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0D FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a0d]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0D FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/A0A484HM70_CAVPO A0A484HM70_CAVPO]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Cavia porcellus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Charlier, P]]		[[Category: Charlier P]]
	[[Category: Herman, R]]		[[Category: Herman R]]
	[[Category: Hilger, C]]		[[Category: Hilger C]]
	[[Category: Janssen-Weets, B]]		[[Category: Janssen-Weets B]]
	[[Category: Swiontek, K]]		[[Category: Swiontek K]]
	~~[[Category: Allergen]]~~
	~~[[Category: Mammalian respiratory allergen~~]]

OCA at 06:45, 31 August 2022

2022-08-31T06:45:56Z

@@ Line 6: / Line 6: @@
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>
 </table>
 __TOC__
 </StructureSection>

OCA at 06:48, 18 August 2022

2022-08-18T06:48:29Z

← Older revision		Revision as of 06:48, 18 August 2022
Line 1:		Line 1:

	==Crystal structure of the major guinea pig allergen Cav p 1.0101 part of the lipocalin family==		==Crystal structure of the major guinea pig allergen Cav p 1.0101 part of the lipocalin family==
	<StructureSection load='8a0d' size='340' side='right'caption='[[8a0d]]' scene=''>		<StructureSection load='8a0d' size='340' side='right'caption='[[8a0d]], [[Resolution\|resolution]] 3.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0D FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a0d]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0D FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>
	</table>		</table>
Line 9:		Line 9:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Charlier P]]		[[Category: Charlier, P]]
	[[Category: Herman R]]		[[Category: Herman, R]]
	[[Category: Hilger C]]		[[Category: Hilger, C]]
	[[Category: Janssen-Weets B]]		[[Category: Janssen-Weets, B]]
	[[Category: Swiontek K]]		[[Category: Swiontek, K]]
			[[Category: Allergen]]
			[[Category: Mammalian respiratory allergen]]

OCA at 04:55, 3 August 2022

2022-08-03T04:55:47Z

← Older revision		Revision as of 04:55, 3 August 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry 8a0d is ON HOLD until Paper Publication~~		==Crystal structure of the major guinea pig allergen Cav p 1.0101 part of the lipocalin family==
			<StructureSection load='8a0d' size='340' side='right'caption='[[8a0d]]' scene=''>
	~~Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0D FirstGlance]. <br>
	~~Description:~~ Crystal structure of the major guinea pig allergen Cav p 1.0101 part of the lipocalin family		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0d OCA], [https://pdbe.org/8a0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0d RCSB], [https://www.ebi.ac.uk/pdbsum/8a0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0d ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Swiontek, K~~]]		__TOC__
	[[Category: ~~Charlier, P~~]]		</StructureSection>
	[[Category: Hilger, C]]		[[Category: Large Structures]]
	[[Category: Janssen-Weets, B]]		[[Category: Charlier P]]
	[[Category: ~~Herman, R~~]]		[[Category: Herman R]]
			[[Category: Hilger C]]
			[[Category: Janssen-Weets B]]
			[[Category: Swiontek K]]

OCA at 06:19, 29 June 2022

2022-06-29T06:19:23Z

← Older revision		Revision as of 06:19, 29 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a0d is ON HOLD		The entry 8a0d is ON HOLD until Paper Publication

	Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K.		Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K.

OCA at 04:04, 15 June 2022

2022-06-15T04:04:52Z

← Older revision		Revision as of 04:04, 15 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a0d is ON HOLD ~~until sometime in the future~~		The entry 8a0d is ON HOLD

	Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K.		Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K.

OCA: Protected "8a0d" [edit=sysop:move=sysop]

2022-06-08T04:21:07Z

Protected "8a0d" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:21, 8 June 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8a0d is ON HOLD until sometime in the future Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K. Description: Crystal st...

2022-06-08T04:21:06Z

New page: '''Unreleased structure''' The entry 8a0d is ON HOLD until sometime in the future Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K. Description: Crystal st...

New page

'''Unreleased structure'''

The entry 8a0d is ON HOLD until sometime in the future

Authors: Herman, R., Charlier, P., Janssen-Weets, B., Hilger, C., Swiontek, K.

Description: Crystal structure of the major guinea pig allergen Cav p 1.0101 part of the lipocalin family
[[Category: Unreleased Structures]]
[[Category: Swiontek, K]]
[[Category: Charlier, P]]
[[Category: Hilger, C]]
[[Category: Janssen-Weets, B]]
[[Category: Herman, R]]