8a0f - Revision history

OCA at 07:00, 21 November 2024

2024-11-21T07:00:32Z

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 == Function ==
 [https://www.uniprot.org/uniprot/DHYS_HUMAN DHYS_HUMAN] Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
 __TOC__
 </StructureSection>

OCA at 08:07, 7 February 2024

2024-02-07T08:07:54Z

← Older revision		Revision as of 08:07, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0F FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.64Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0f OCA], [https://pdbe.org/8a0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0f RCSB], [https://www.ebi.ac.uk/pdbsum/8a0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0f OCA], [https://pdbe.org/8a0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0f RCSB], [https://www.ebi.ac.uk/pdbsum/8a0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/DHYS_HUMAN DHYS_HUMAN] Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.		[https://www.uniprot.org/uniprot/DHYS_HUMAN DHYS_HUMAN] Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Hypusination is a unique post-translational modification of the eukaryotic translation factor 5A (eIF5A) that is essential for overcoming ribosome stalling at polyproline sequence stretches. The initial step of hypusination, the formation of deoxyhypusine, is catalyzed by deoxyhypusine synthase (DHS), however, the molecular details of the DHS-mediated reaction remained elusive. Recently, patient-derived variants of DHS and eIF5A have been linked to rare neurodevelopmental disorders. Here, we present the cryo-EM structure of the human eIF5A-DHS complex at 2.8 A resolution and a crystal structure of DHS trapped in the key reaction transition state. Furthermore, we show that disease-associated DHS variants influence the complex formation and hypusination efficiency. Hence, our work dissects the molecular details of the deoxyhypusine synthesis reaction and reveals how clinically-relevant mutations affect this crucial cellular process.

	Cryo-EM structure of human eIF5A-DHS complex reveals the molecular basis of hypusination-associated neurodegenerative disorders.,Wator E, Wilk P, Biela A, Rawski M, Zak KM, Steinchen W, Bange G, Glatt S, Grudnik P Nat Commun. 2023 Mar 27;14(1):1698. doi: 10.1038/s41467-023-37305-2. PMID:36973244<ref>PMID:36973244</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 8a0f" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:26, 7 April 2023

2023-04-07T06:26:01Z

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-The entry 8a0f is ON HOLD  until Paper Publication
+==Crystal structure of human deoxyhypusine synthase variant K329A in complex with NAD and SPD==
-Authors:
+Cryo-EM structure of human eIF5A-DHS complex reveals the molecular basis of hypusination-associated neurodegenerative disorders.,Wator E, Wilk P, Biela A, Rawski M, Zak KM, Steinchen W, Bange G, Glatt S, Grudnik P Nat Commun. 2023 Mar 27;14(1):1698. doi: 10.1038/s41467-023-37305-2. PMID:36973244<ref>PMID:36973244</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>

OCA at 04:10, 20 July 2022

2022-07-20T04:10:08Z

← Older revision		Revision as of 04:10, 20 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a0f is ON HOLD		The entry 8a0f is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8a0f" [edit=sysop:move=sysop]

2022-06-08T04:21:17Z

Protected "8a0f" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:21, 8 June 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8a0f is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-08T04:21:16Z

New page: '''Unreleased structure''' The entry 8a0f is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8a0f is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]