8a1e - Revision history

OCA at 14:19, 6 November 2024

2024-11-06T14:19:15Z

← Older revision		Revision as of 14:19, 6 November 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a1e]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rabies_virus_strain_Pasteur_vaccin Rabies virus strain Pasteur vaccin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A1E FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a1e]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rabies_virus_strain_Pasteur_vaccin Rabies virus strain Pasteur vaccin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A1E FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a1e OCA], [https://pdbe.org/8a1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a1e RCSB], [https://www.ebi.ac.uk/pdbsum/8a1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a1e ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.83Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a1e OCA], [https://pdbe.org/8a1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a1e RCSB], [https://www.ebi.ac.uk/pdbsum/8a1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a1e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/GLYCO_RABVP GLYCO_RABVP]] Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity).		[https://www.uniprot.org/uniprot/GLYCO_RABVP GLYCO_RABVP] Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Rabies virus (RABV) causes lethal encephalitis and is responsible for approximately 60,000 deaths per year. As the sole virion-surface protein, the rabies virus glycoprotein (RABV-G) mediates host-cell entry. RABV-G's pre-fusion trimeric conformation displays epitopes bound by protective neutralizing antibodies that can be induced by vaccination or passively administered for post-exposure prophylaxis. We report a 2.8-A structure of a RABV-G trimer in the pre-fusion conformation, in complex with two neutralizing and protective monoclonal antibodies, 17C7 and 1112-1, that recognize distinct epitopes. One of these antibodies is a licensed prophylactic (17C7, Rabishield), which we show locks the protein in pre-fusion conformation. Targeted mutations can similarly stabilize RABV-G in the pre-fusion conformation, a key step toward structure-guided vaccine design. These data reveal the higher-order architecture of a key therapeutic target and the structural basis of neutralization by antibodies binding two key antigenic sites, and this will facilitate the development of improved vaccines and prophylactic antibodies.		Rabies virus (RABV) causes lethal encephalitis and is responsible for approximately 60,000 deaths per year. As the sole virion-surface protein, the rabies virus glycoprotein (RABV-G) mediates host-cell entry. RABV-G's pre-fusion trimeric conformation displays epitopes bound by protective neutralizing antibodies that can be induced by vaccination or passively administered for post-exposure prophylaxis. We report a 2.8-A structure of a RABV-G trimer in the pre-fusion conformation, in complex with two neutralizing and protective monoclonal antibodies, 17C7 and 1112-1, that recognize distinct epitopes. One of these antibodies is a licensed prophylactic (17C7, Rabishield), which we show locks the protein in pre-fusion conformation. Targeted mutations can similarly stabilize RABV-G in the pre-fusion conformation, a key step toward structure-guided vaccine design. These data reveal the higher-order architecture of a key therapeutic target and the structural basis of neutralization by antibodies binding two key antigenic sites, and this will facilitate the development of improved vaccines and prophylactic antibodies.

	Structure of trimeric pre-fusion rabies virus glycoprotein in complex with two protective antibodies.,Ng WM, Fedosyuk S, English S, Augusto G, Berg A, Thorley L, Haselon AS, Segireddy RR, Bowden TA, Douglas AD Cell Host Microbe. 2022 ~~Aug 11. pii: S1931-3128~~(22)~~00360~~-2. doi:, 10.1016/j.chom.2022.07.014. PMID:35985336<ref>PMID:35985336</ref>		Structure of trimeric pre-fusion rabies virus glycoprotein in complex with two protective antibodies.,Ng WM, Fedosyuk S, English S, Augusto G, Berg A, Thorley L, Haselon AS, Segireddy RR, Bowden TA, Douglas AD Cell Host Microbe. 2022 Sep 14;30(9):1219-1230.e7. doi: , 10.1016/j.chom.2022.07.014. Epub 2022 Aug 18. PMID:35985336<ref>PMID:35985336</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	<div class="pdbe-citations 8a1e" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 8a1e" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:54, 7 September 2022

2022-09-07T19:54:49Z

@@ Line 1: / Line 1: @@
-====
+==Rabies virus glycoprotein in complex with Fab fragments of 17C7 and 1112-1 neutralizing antibodies==
-<StructureSection load='8a1e' size='340' side='right'caption='[[8a1e]]' scene=''>
+<StructureSection load='8a1e' size='340' side='right'caption='[[8a1e]], [[Resolution|resolution]] 2.83&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
+<table><tr><td colspan='2'>[[8a1e]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rabies_virus_strain_Pasteur_vaccin Rabies virus strain Pasteur vaccin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A1E FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a1e OCA], [https://pdbe.org/8a1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a1e RCSB], [https://www.ebi.ac.uk/pdbsum/8a1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a1e ProSAT]</span></td></tr>
 </table>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Z-disk]]
+[[Category: Mus musculus]]

OCA at 06:25, 18 August 2022

2022-08-18T06:25:14Z

← Older revision		Revision as of 06:25, 18 August 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8a1e is ~~ON HOLD~~		====
			<StructureSection load='8a1e' size='340' side='right'caption='[[8a1e]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a1e OCA], [https://pdbe.org/8a1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a1e RCSB], [https://www.ebi.ac.uk/pdbsum/8a1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a1e ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA: Protected "8a1e" [edit=sysop:move=sysop]

2022-06-08T04:23:01Z

Protected "8a1e" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:23, 8 June 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8a1e is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-08T04:23:00Z

New page: '''Unreleased structure''' The entry 8a1e is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8a1e is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]