8a63 - Revision history

OCA at 07:00, 21 November 2024

2024-11-21T07:00:51Z

← Older revision		Revision as of 07:00, 21 November 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a63]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A63 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a63]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A63 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a63 OCA], [https://pdbe.org/8a63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a63 RCSB], [https://www.ebi.ac.uk/pdbsum/8a63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a63 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a63 OCA], [https://pdbe.org/8a63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a63 RCSB], [https://www.ebi.ac.uk/pdbsum/8a63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a63 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RL28_LISMO RL28_LISMO]		[https://www.uniprot.org/uniprot/RL28_LISMO RL28_LISMO]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			HflX is a ubiquitous bacterial GTPase that splits and recycles stressed ribosomes. In addition to HflX, Listeria monocytogenes contains a second HflX homolog, HflXr. Unlike HflX, HflXr confers resistance to macrolide and lincosamide antibiotics by an experimentally unexplored mechanism. Here, we have determined cryo-EM structures of L. monocytogenes HflXr-50S and HflX-50S complexes as well as L. monocytogenes 70S ribosomes in the presence and absence of the lincosamide lincomycin. While the overall geometry of HflXr on the 50S subunit is similar to that of HflX, a loop within the N-terminal domain of HflXr, which is two amino acids longer than in HflX, reaches deeper into the peptidyltransferase center. Moreover, unlike HflX, the binding of HflXr induces conformational changes within adjacent rRNA nucleotides that would be incompatible with drug binding. These findings suggest that HflXr confers resistance using an allosteric ribosome protection mechanism, rather than by simply splitting and recycling antibiotic-stalled ribosomes.

			Structural basis for HflXr-mediated antibiotic resistance in Listeria monocytogenes.,Koller TO, Turnbull KJ, Vaitkevicius K, Crowe-McAuliffe C, Roghanian M, Bulvas O, Nakamoto JA, Kurata T, Julius C, Atkinson GC, Johansson J, Hauryliuk V, Wilson DN Nucleic Acids Res. 2022 Oct 28;50(19):11285-11300. doi: 10.1093/nar/gkac934. PMID:36300626<ref>PMID:36300626</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8a63" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:23, 3 November 2022

2022-11-03T07:23:20Z

← Older revision		Revision as of 07:23, 3 November 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 8a63 is ~~ON HOLD until 2024~~-06-16		==Cryo-EM structure of Listeria monocytogenes 50S ribosomal subunit.==
			<StructureSection load='8a63' size='340' side='right'caption='[[8a63]], [[Resolution\|resolution]] 3.10Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[8a63]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A63 FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a63 OCA], [https://pdbe.org/8a63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a63 RCSB], [https://www.ebi.ac.uk/pdbsum/8a63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a63 ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/RL28_LISMO RL28_LISMO]
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Listeria monocytogenes EGD-e]]
			[[Category: Crowe-McAuliffe C]]
			[[Category: Koller TO]]
			[[Category: Wilson DN]]

OCA at 03:45, 3 August 2022

2022-08-03T03:45:06Z

← Older revision		Revision as of 03:45, 3 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a63 is ON HOLD		The entry 8a63 is ON HOLD until 2024-06-16

	Authors:		Authors:

OCA: Protected "8a63" [edit=sysop:move=sysop]

2022-06-22T07:22:22Z

Protected "8a63" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:22, 22 June 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8a63 is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-22T07:22:21Z

New page: '''Unreleased structure''' The entry 8a63 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8a63 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]