8a8f - Revision history

OCA at 05:17, 12 June 2024

2024-06-12T05:17:06Z

← Older revision		Revision as of 05:17, 12 June 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a8f OCA], [https://pdbe.org/8a8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a8f RCSB], [https://www.ebi.ac.uk/pdbsum/8a8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a8f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a8f OCA], [https://pdbe.org/8a8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a8f RCSB], [https://www.ebi.ac.uk/pdbsum/8a8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a8f ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	[https://www.uniprot.org/uniprot/PP12_YEAST PP12_YEAST] Involved in control of glycogen metabolism, meiosis, translation, chromosome segregation, cell polarity and G2/M cell cycle progression. PP1 may act in opposition to the IPL1 protein kinase in regulating chromosome segregation by ~~dephosphorylating H3S10ph. The BUD14-GLC7 complex~~ is ~~necessary~~ to ~~regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex. Component of~~ the cleavage and polyadenylation factor (CPF) ~~complex~~, which ~~plays a key role in polyadenylation-dependent~~ pre-~~mRNA~~ 3'-~~end~~ formation ~~and cooperates~~ with ~~cleavage~~ factors including the ~~CFIA complex~~ and ~~NAB4~~/~~CFIB~~. ~~Component~~ of the ~~APT complex, which may be involved in polyadenylation~~-~~independent transcript 3'~~-~~end formation.~~		== Publication Abstract from PubMed ==
			Transcription termination by RNA polymerase II (RNA Pol II) is linked to RNA 3' end processing by the cleavage and polyadenylation factor (CPF or CPSF). CPF contains endonuclease, poly(A) polymerase, and protein phosphatase activities, which cleave and polyadenylate pre-mRNAs and dephosphorylate RNA Pol II to control transcription. Exactly how the RNA 3' end processing machinery is coupled to transcription remains unclear. Here, we combine in vitro reconstitution, structural studies, and genome-wide analyses to show that yeast CPF physically and functionally interacts with RNA Pol II. Surprisingly, CPF-mediated dephosphorylation promotes the formation of an RNA Pol II stalk-to-stalk homodimer in vitro. This dimer is compatible with transcription but not with the binding of transcription elongation factors. Disruption of the dimerization interface in cells causes transcription defects, including altered RNA Pol II abundance on protein-coding genes, tRNA genes, and intergenic regions. We hypothesize that RNA Pol II dimerization may provide a mechanistic basis for the allosteric model of transcription termination.

			A direct interaction between CPF and RNA Pol II links RNA 3' end processing to transcription.,Carminati M, Rodriguez-Molina JB, Manav MC, Bellini D, Passmore LA Mol Cell. 2023 Dec 21;83(24):4461-4478.e13. doi: 10.1016/j.molcel.2023.11.004. , Epub 2023 Nov 28. PMID:38029752<ref>PMID:38029752</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8a8f" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:50, 29 November 2023

2023-11-29T13:50:35Z

← Older revision		Revision as of 13:50, 29 November 2023
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	~~'''Unreleased structure'''~~

	The ~~entry 8a8f~~ is ~~ON HOLD~~ ~~until Paper Publication~~		==Crystal structure of Glc7 phosphatase in complex with the regulatory region of Ref2==
			<StructureSection load='8a8f' size='340' side='right'caption='[[8a8f]], [[Resolution\|resolution]] 1.85Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[8a8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A8F FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a8f OCA], [https://pdbe.org/8a8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a8f RCSB], [https://www.ebi.ac.uk/pdbsum/8a8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a8f ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PP12_YEAST PP12_YEAST] Involved in control of glycogen metabolism, meiosis, translation, chromosome segregation, cell polarity and G2/M cell cycle progression. PP1 may act in opposition to the IPL1 protein kinase in regulating chromosome segregation by dephosphorylating H3S10ph. The BUD14-GLC7 complex is necessary to regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex. Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation.
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Saccharomyces cerevisiae]]
			[[Category: Bellini D]]
			[[Category: Carminati M]]
			[[Category: Manav CM]]
			[[Category: Passmore LA]]

OCA at 03:45, 3 August 2022

2022-08-03T03:45:29Z

← Older revision		Revision as of 03:45, 3 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a8f is ON HOLD		The entry 8a8f is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8a8f" [edit=sysop:move=sysop]

2022-06-29T06:23:34Z

Protected "8a8f" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:23, 29 June 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8a8f is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-29T06:23:33Z

New page: '''Unreleased structure''' The entry 8a8f is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8a8f is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]