8a93 - Revision history

OCA at 06:44, 24 July 2024

2024-07-24T06:44:12Z

← Older revision		Revision as of 06:44, 24 July 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a93]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A93 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a93]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A93 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.05Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a93 OCA], [https://pdbe.org/8a93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a93 RCSB], [https://www.ebi.ac.uk/pdbsum/8a93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a93 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a93 OCA], [https://pdbe.org/8a93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a93 RCSB], [https://www.ebi.ac.uk/pdbsum/8a93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a93 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q5SLM9_THET8 Q5SLM9_THET8] The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.[HAMAP-Rule:MF_00365]		[https://www.uniprot.org/uniprot/Q5SLM9_THET8 Q5SLM9_THET8] The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.[HAMAP-Rule:MF_00365]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			In bacteria, one type of homologous-recombination-based DNA-repair pathway involves RecFOR proteins that bind at the junction between single-stranded (ss) and double-stranded (ds) DNA. They facilitate the replacement of SSB protein, which initially covers ssDNA, with RecA, which mediates the search for homologous sequences. However, the molecular mechanism of RecFOR cooperation remains largely unknown. We used Thermus thermophilus proteins to study this system. Here, we present a cryo-electron microscopy structure of the RecF-dsDNA complex, and another reconstruction that shows how RecF interacts with two different regions of the tetrameric RecR ring. Lower-resolution reconstructions of the RecR-RecO subcomplex and the RecFOR-DNA assembly explain how RecO is positioned to interact with ssDNA and SSB, which is proposed to lock the complex on a ssDNA-dsDNA junction. Our results integrate the biochemical data available for the RecFOR system and provide a framework for its complete understanding.

			Mechanism of RecF-RecO-RecR cooperation in bacterial homologous recombination.,Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M Nat Struct Mol Biol. 2023 May;30(5):650-660. doi: 10.1038/s41594-023-00967-z. , Epub 2023 Apr 20. PMID:37081315<ref>PMID:37081315</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8a93" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:03, 26 April 2023

2023-04-26T17:03:06Z

@@ Line 1: / Line 1: @@
-The entry 8a93 is ON HOLD  until Paper Publication
+==Complex of RecF-RecR-DNA from Thermus thermophilus.==
+<StructureSection load='8a93' size='340' side='right'caption='[[8a93]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8a93]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A93 FirstGlance]. <br>
-Description:
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a93 OCA], [https://pdbe.org/8a93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a93 RCSB], [https://www.ebi.ac.uk/pdbsum/8a93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a93 ProSAT]</span></td></tr>

OCA at 09:04, 1 February 2023

2023-02-01T09:04:04Z

← Older revision		Revision as of 09:04, 1 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a93 is ON HOLD		The entry 8a93 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:47, 25 January 2023

2023-01-25T04:47:32Z

← Older revision		Revision as of 04:47, 25 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a93 is ON HOLD ~~until Paper Publication~~		The entry 8a93 is ON HOLD

	Authors:		Authors:

OCA at 03:41, 25 August 2022

2022-08-25T03:41:41Z

← Older revision		Revision as of 03:41, 25 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8a93 is ON HOLD		The entry 8a93 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8a93" [edit=sysop:move=sysop]

2022-07-06T15:04:57Z

Protected "8a93" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:04, 6 July 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8a93 is ON HOLD Authors: Description: Category: Unreleased Structures

2022-07-06T15:04:56Z

New page: '''Unreleased structure''' The entry 8a93 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8a93 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]