8a9d - Revision history

OCA at 14:19, 6 November 2024

2024-11-06T14:19:48Z

← Older revision		Revision as of 14:19, 6 November 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The utility of X-ray crystal structures determined under ambient-temperature conditions is becoming increasingly recognized. Such experiments can allow protein dynamics to be characterized and are particularly well suited to challenging protein targets that may form fragile crystals that are difficult to cryo-cool. Room-temperature data collection also enables time-resolved experiments. In contrast to the high-throughput highly automated pipelines for determination of structures at cryogenic temperatures widely available at synchrotron beamlines, room-temperature methodology is less mature. Here, the current status of the fully automated ambient-temperature beamline VMXi at Diamond Light Source is described, and a highly efficient pipeline from protein sample to final multi-crystal data analysis and structure determination is shown. The capability of the pipeline is illustrated using a range of user case studies representing different challenges, and from high and lower symmetry space groups and varied crystal sizes. It is also demonstrated that very rapid structure determination from crystals in situ within crystallization plates is now routine with minimal user intervention.

			Protein-to-structure pipeline for ambient-temperature in situ crystallography at VMXi.,Mikolajek H, Sanchez-Weatherby J, Sandy J, Gildea RJ, Campeotto I, Cheruvara H, Clarke JD, Foster T, Fujii S, Paulsen IT, Shah BS, Hough MA IUCrJ. 2023 Jul 1;10(Pt 4):420-429. doi: 10.1107/S2052252523003810. PMID:37199504<ref>PMID:37199504</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8a9d" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:19, 19 July 2023

2023-07-19T06:19:45Z

← Older revision		Revision as of 06:19, 19 July 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a9d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9D FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a9d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9d OCA], [https://pdbe.org/8a9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9d RCSB], [https://www.ebi.ac.uk/pdbsum/8a9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9d OCA], [https://pdbe.org/8a9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9d RCSB], [https://www.ebi.ac.uk/pdbsum/8a9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>

			==See Also==
			*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:08, 18 May 2023

2023-05-18T07:08:50Z

← Older revision		Revision as of 07:08, 18 May 2023
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	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:06, 8 September 2022

2022-09-08T05:06:30Z

← Older revision		Revision as of 05:06, 8 September 2022
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	==Multicrystal room temperature structure of Lysozyme collected using a double multilayer monochromator.==		==Multicrystal room temperature structure of Lysozyme collected using a double multilayer monochromator.==
	<StructureSection load='8a9d' size='340' side='right'caption='[[8a9d]]' scene=''>		<StructureSection load='8a9d' size='340' side='right'caption='[[8a9d]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9D FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a9d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9D FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9d OCA], [https://pdbe.org/8a9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9d RCSB], [https://www.ebi.ac.uk/pdbsum/8a9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9d ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9d OCA], [https://pdbe.org/8a9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9d RCSB], [https://www.ebi.ac.uk/pdbsum/8a9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9d ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cheruvara H]]		[[Category: Cheruvara H]]

OCA at 06:58, 20 July 2022

2022-07-20T06:58:56Z

← Older revision		Revision as of 06:58, 20 July 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8a9d is ~~ON HOLD~~		==Multicrystal room temperature structure of Lysozyme collected using a double multilayer monochromator.==
			<StructureSection load='8a9d' size='340' side='right'caption='[[8a9d]]' scene=''>
	~~Authors~~: ~~Sandy~~, J., ~~Cheruvara~~, H., ~~Mikolajek~~, H.~~, Sanchez~~-~~Weatherby, J~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9D FirstGlance]. <br>
	~~Description: Multicrystal room temperature structure of Lysozyme collected using a double multilayer monochromator.~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9d OCA], [https://pdbe.org/8a9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9d RCSB], [https://www.ebi.ac.uk/pdbsum/8a9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9d ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Cheruvara, H]]		__TOC__
	[[Category: ~~Sandy, J~~]]		</StructureSection>
	[[Category: Sanchez-Weatherby, J]]		[[Category: Large Structures]]
	[[Category: ~~Mikolajek, H~~]]		[[Category: Cheruvara H]]
			[[Category: Mikolajek H]]
			[[Category: Sanchez-Weatherby J]]
			[[Category: Sandy J]]

OCA: Protected "8a9d" [edit=sysop:move=sysop]

2022-07-06T15:05:28Z

Protected "8a9d" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:05, 6 July 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8a9d is ON HOLD Authors: Sandy, J., Cheruvara, H., Mikolajek, H., Sanchez-Weatherby, J. Description: Multicrystal room temperature structure of Lys...

2022-07-06T15:05:27Z

New page: '''Unreleased structure''' The entry 8a9d is ON HOLD Authors: Sandy, J., Cheruvara, H., Mikolajek, H., Sanchez-Weatherby, J. Description: Multicrystal room temperature structure of Lys...

New page

'''Unreleased structure'''

The entry 8a9d is ON HOLD

Authors: Sandy, J., Cheruvara, H., Mikolajek, H., Sanchez-Weatherby, J.

Description: Multicrystal room temperature structure of Lysozyme collected using a double multilayer monochromator.
[[Category: Unreleased Structures]]
[[Category: Cheruvara, H]]
[[Category: Sandy, J]]
[[Category: Sanchez-Weatherby, J]]
[[Category: Mikolajek, H]]