8aew - Revision history

OCA at 09:26, 17 October 2024

2024-10-17T09:26:24Z

← Older revision		Revision as of 09:26, 17 October 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aew OCA], [https://pdbe.org/8aew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aew RCSB], [https://www.ebi.ac.uk/pdbsum/8aew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aew ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aew OCA], [https://pdbe.org/8aew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aew RCSB], [https://www.ebi.ac.uk/pdbsum/8aew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aew ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https~~://~~www~~.~~uniprot~~.~~org~~/~~uniprot~~/~~A9WIU3_CHLAA A9WIU3_CHLAA]~~		== Publication Abstract from PubMed ==
			Malonyl-Coenzyme A Reductase (MCR) in Chloroflexus aurantiacus, a characteristic enzyme of the 3-hydroxypropionate (3-HP) cycle, catalyses the reduction of malonyl-CoA to 3-HP. MCR is a bi-functional enzyme; in the first step, malonyl-CoA is reduced to the free intermediate malonate semialdehyde by the C-terminal region of MCR, and this is further reduced to 3-HP by the N-terminal region of MCR. Here we present the crystal structures of both N-terminal and C-terminal regions of the MCR from C. aurantiacus. A catalytic mechanism is suggested by ligand and substrate bound structures, and structural and kinetic studies of MCR variants. Both MCR structures reveal one catalytic, and one non-catalytic SDR (short chain dehydrogenase/reductase) domain. C-terminal MCR has a lid domain which undergoes a conformational change and controls the reaction. In the proposed mechanism of the C-terminal MCR, the conversion of malonyl-CoA to malonate semialdehyde is based on the reduction of malonyl-CoA by NADPH, followed by the decomposition of the hemithioacetal to produce malonate semialdehyde and coenzyme A. Conserved arginines, Arg734 and Arg773 are proposed to play key roles in the mechanism and conserved Ser719, and Tyr737 are other essential residues forming an oxyanion hole for the substrate intermediates.

			Dynamic lid domain of Chloroflexus aurantiacus Malonyl-CoA reductase controls the reaction.,Kabasakal BV, Cotton CAR, Murray JW Biochimie. 2024 Apr;219:12-20. doi: 10.1016/j.biochi.2023.11.003. Epub 2023 Nov , 10. PMID:37952891<ref>PMID:37952891</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8aew" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:06, 22 November 2023

2023-11-22T11:06:55Z

← Older revision		Revision as of 11:06, 22 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8aew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_J-10-fl Chloroflexus aurantiacus J-10-fl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AEW FirstGlance]. <br>		<table><tr><td colspan='2'>[[8aew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_J-10-fl Chloroflexus aurantiacus J-10-fl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AEW FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aew OCA], [https://pdbe.org/8aew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aew RCSB], [https://www.ebi.ac.uk/pdbsum/8aew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aew ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.72Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aew OCA], [https://pdbe.org/8aew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aew RCSB], [https://www.ebi.ac.uk/pdbsum/8aew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aew ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:26, 7 April 2023

2023-04-07T06:26:17Z

← Older revision		Revision as of 06:26, 7 April 2023
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8aew is ~~ON HOLD until Paper Publication~~		==Malonyl-CoA reductase from Chloroflexus aurantiacus - N-terminal Apo==
			<StructureSection load='8aew' size='340' side='right'caption='[[8aew]], [[Resolution\|resolution]] 2.72Å' scene=''>
	~~Authors~~: ~~Kabasakal~~, B.V., ~~Murray~~, J.W.		== Structural highlights ==
			<table><tr><td colspan='2'>[[8aew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_J-10-fl Chloroflexus aurantiacus J-10-fl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AEW FirstGlance]. <br>
	~~Description~~: ~~Malonyl-CoA reductase from~~ Chloroflexus aurantiacus -N-~~terminal Apo~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aew OCA], [https://pdbe.org/8aew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aew RCSB], [https://www.ebi.ac.uk/pdbsum/8aew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aew ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Kabasakal~~, B.V~~]]		== Function ==
	[[Category: Murray~~, J.W~~]]		[https://www.uniprot.org/uniprot/A9WIU3_CHLAA A9WIU3_CHLAA]
			__TOC__
			</StructureSection>
			[[Category: Chloroflexus aurantiacus J-10-fl]]
			[[Category: Large Structures]]
			[[Category: Kabasakal BV]]
			[[Category: Murray JW]]

OCA at 03:43, 18 August 2022

2022-08-18T03:43:51Z

← Older revision		Revision as of 03:43, 18 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8aew is ON HOLD		The entry 8aew is ON HOLD until Paper Publication

	Authors: Kabasakal, B.V., Murray, J.W.		Authors: Kabasakal, B.V., Murray, J.W.

OCA: Protected "8aew" [edit=sysop:move=sysop]

2022-07-20T04:20:18Z

Protected "8aew" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:20, 20 July 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8aew is ON HOLD Authors: Kabasakal, B.V., Murray, J.W. Description: Malonyl-CoA reductase from Chloroflexus aurantiacus -N-terminal Apo [[Category:...

2022-07-20T04:20:16Z

New page: '''Unreleased structure''' The entry 8aew is ON HOLD Authors: Kabasakal, B.V., Murray, J.W. Description: Malonyl-CoA reductase from Chloroflexus aurantiacus -N-terminal Apo [[Category:...

New page

'''Unreleased structure'''

The entry 8aew is ON HOLD

Authors: Kabasakal, B.V., Murray, J.W.

Description: Malonyl-CoA reductase from Chloroflexus aurantiacus -N-terminal Apo
[[Category: Unreleased Structures]]
[[Category: Kabasakal, B.V]]
[[Category: Murray, J.W]]