8ast - Revision history

OCA at 14:21, 6 November 2024

2024-11-06T14:21:38Z

← Older revision		Revision as of 14:21, 6 November 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ast FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ast OCA], [https://pdbe.org/8ast PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ast RCSB], [https://www.ebi.ac.uk/pdbsum/8ast PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ast ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ast FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ast OCA], [https://pdbe.org/8ast PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ast RCSB], [https://www.ebi.ac.uk/pdbsum/8ast PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ast ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https~~://~~www~~.~~uniprot~~.~~org~~/~~uniprot~~/~~A0A0N1ABZ3_9HYPH A0A0N1ABZ3_9HYPH]~~		== Publication Abstract from PubMed ==
			The dimeric avidin family has been expanded in recent years to include many new members. All of them lack the intermonomeric Trp that plays a critical role in biotin-binding. Nevertheless, these new members of the avidins maintain the high affinity towards biotin. Additionally, all of the dimeric avidins share a very unique property: namely, the cylindrical oligomerization in the crystal structure. The newest member described here, agroavidin from the agrobacterium, Rhizobium sp. AAP43, shares their important structural features. However, the affinity of agroavidin towards biotin is lower than all other members of the avidin family, due to the presence of phenylalanine instead of a conserved tyrosine in the biotin-binding site. Mutating this phenylalanine into tyrosine regenerated the high affinity, which emphasizes the importance of this particular tyrosine residue. Another unique feature that distinguishes agroavidin from the other dimeric avidins is that it does not produce oligomers in its crystal structure. In order to understand the factors that promote oligomerization in dimeric avidins, we exchanged the C-terminal region of agroavidin with that of hoefavidin that produced octamers. This exchange resulted in a decamer rather than an octamer. This unusual outcome demonstrates the impact of the C-terminal region on the ability to produce oligomers. The decameric assembly of agroavidin expands the avidin-biotin toolbox even further and could well pave the path into new biotin-based technologies. Moreover, uncovering the factors that induce dimeric avidins into oligomeric assemblies may aid in better understanding the general molecular determinants that promote oligomerization.

			Self-assembly of a dimeric avidin into unique higher-order oligomers.,Bana J, Warwar J, Bayer EA, Livnah O FEBS J. 2023 Jul;290(14):3563-3579. doi: 10.1111/febs.16764. Epub 2023 Mar 10. PMID:36853192<ref>PMID:36853192</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8ast" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:13, 7 February 2024

2024-02-07T08:13:32Z

@@ Line 9: / Line 9: @@
 == Function ==
 [https://www.uniprot.org/uniprot/A0A0N1ABZ3_9HYPH A0A0N1ABZ3_9HYPH]
 __TOC__
 </StructureSection>

OCA at 11:10, 2 August 2023

2023-08-02T11:10:25Z

← Older revision		Revision as of 11:10, 2 August 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8ast]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._AAP43 Rhizobium sp. AAP43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AST FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ast]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._AAP43 Rhizobium sp. AAP43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AST FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ast FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ast OCA], [https://pdbe.org/8ast PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ast RCSB], [https://www.ebi.ac.uk/pdbsum/8ast PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ast ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ast FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ast OCA], [https://pdbe.org/8ast PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ast RCSB], [https://www.ebi.ac.uk/pdbsum/8ast PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ast ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 20:29, 12 April 2023

2023-04-12T20:29:53Z

← Older revision		Revision as of 20:29, 12 April 2023
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	The ~~entry~~ 8ast is ~~ON HOLD until Paper~~ Publication		==The Crystal structure of short F46Y agroavidin - apo==
			<StructureSection load='8ast' size='340' side='right'caption='[[8ast]], [[Resolution\|resolution]] 1.55Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8ast]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._AAP43 Rhizobium sp. AAP43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AST FirstGlance]. <br>
			</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ast FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ast OCA], [https://pdbe.org/8ast PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ast RCSB], [https://www.ebi.ac.uk/pdbsum/8ast PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ast ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A0N1ABZ3_9HYPH A0A0N1ABZ3_9HYPH]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The dimeric avidin family has been expanded in recent years to include many new members. All of them lack the intermonomeric Trp that plays a critical role in biotin-binding. Nevertheless, these new members of the avidins maintain the high affinity towards biotin. Additionally, all of the dimeric avidins share a very unique property: namely, the cylindrical oligomerization in the crystal structure. The newest member described here, agroavidin from the agrobacterium, Rhizobium sp. AAP43, shares their important structural features. However, the affinity of agroavidin towards biotin is lower than all other members of the avidin family, due to the presence of phenylalanine instead of a conserved tyrosine in the biotin-binding site. Mutating this phenylalanine into tyrosine regenerated the high affinity, which emphasizes the importance of this particular tyrosine residue. Another unique feature that distinguishes agroavidin from the other dimeric avidins is that it does not produce oligomers in its crystal structure. In order to understand the factors that promote oligomerization in dimeric avidins, we exchanged the C-terminal region of agroavidin with that of hoefavidin that produced octamers. This exchange resulted in a decamer rather than an octamer. This unusual outcome demonstrates the impact of the C-terminal region on the ability to produce oligomers. The decameric assembly of agroavidin expands the avidin-biotin toolbox even further and could well pave the path into new biotin-based technologies. Moreover, uncovering the factors that induce dimeric avidins into oligomeric assemblies may aid in better understanding the general molecular determinants that promote oligomerization.

	~~Authors: Livnah, O~~., Bana, J.		Self-assembly of a dimeric avidin into unique higher-order oligomers.,Bana J, Warwar J, Bayer EA, Livnah O FEBS J. 2023 Feb 28. doi: 10.1111/febs.16764. PMID:36853192<ref>PMID:36853192</ref>

	~~Description: The Crystal structure~~ of ~~short F46Y agroavidin~~ -~~apo~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: ~~Livnah, O~~]]		<div class="pdbe-citations 8ast" style="background-color:#fffaf0;"></div>
	[[Category: Bana, J]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Rhizobium sp. AAP43]]
			[[Category: Bana J]]
			[[Category: Livnah O]]

OCA at 04:31, 28 September 2022

2022-09-28T04:31:18Z

← Older revision		Revision as of 04:31, 28 September 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8ast is ON HOLD		The entry 8ast is ON HOLD until Paper Publication

	Authors: Livnah, O., Bana, J.		Authors: Livnah, O., Bana, J.
Line 7:		Line 7:
	Description: The Crystal structure of short F46Y agroavidin -apo		Description: The Crystal structure of short F46Y agroavidin -apo
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Livnah, O]]
	[[Category: Bana, J]]		[[Category: Bana, J]]
	~~[[Category: Livnah, O]]~~

OCA: Protected "8ast" [edit=sysop:move=sysop]

2022-08-31T05:26:06Z

Protected "8ast" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:26, 31 August 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8ast is ON HOLD Authors: Livnah, O., Bana, J. Description: The Crystal structure of short F46Y agroavidin -apo Category: Unreleased Structures ...

2022-08-31T05:26:05Z

New page: '''Unreleased structure''' The entry 8ast is ON HOLD Authors: Livnah, O., Bana, J. Description: The Crystal structure of short F46Y agroavidin -apo Category: Unreleased Structures ...

New page

'''Unreleased structure'''

The entry 8ast is ON HOLD

Authors: Livnah, O., Bana, J.

Description: The Crystal structure of short F46Y agroavidin -apo
[[Category: Unreleased Structures]]
[[Category: Bana, J]]
[[Category: Livnah, O]]