8b4e - Revision history

OCA at 06:26, 19 June 2024

2024-06-19T06:26:32Z

← Older revision		Revision as of 06:26, 19 June 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/TOXR_VIBCH TOXR_VIBCH] This transcription activator controls cholera toxin, pilus colonization factor and outer membrane protein expression in V.cholerae. It binds to the 5'-TTTTGAT-3' tandemly repeated DNA sequence in the cholera toxin promoter region. ToxS interacts with the C-terminal periplasmic domain of ToxR, stimulating its activity. It activates transcription at the promoters for tcpI and tcpA and this is presumably via ToxT.		[https://www.uniprot.org/uniprot/TOXR_VIBCH TOXR_VIBCH] This transcription activator controls cholera toxin, pilus colonization factor and outer membrane protein expression in V.cholerae. It binds to the 5'-TTTTGAT-3' tandemly repeated DNA sequence in the cholera toxin promoter region. ToxS interacts with the C-terminal periplasmic domain of ToxR, stimulating its activity. It activates transcription at the promoters for tcpI and tcpA and this is presumably via ToxT.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	ToxR, a Vibrio cholerae transmembrane one-component signal transduction factor, lies within a regulatory cascade that results in the expression of ToxT, toxin coregulated pilus, and cholera toxin. While ToxR has been extensively studied for its ability to activate or repress various genes in V. cholerae, here we present the crystal structures of the ToxR cytoplasmic domain bound to DNA at the toxT and ompU promoters. The structures confirm some predicted interactions, yet reveal other unexpected promoter interactions with implications for other potential regulatory roles for ToxR. We show that ToxR is a versatile virulence regulator that recognizes diverse and extensive, eukaryotic-like regulatory DNA sequences, that relies more on DNA structural elements than specific sequences for binding. Using this topological DNA recognition mechanism, ToxR can bind both in tandem and in a twofold inverted-repeat-driven manner. Its regulatory action is based on coordinated multiple binding to promoter regions near the transcription start site, which can remove the repressing H-NS proteins and prepares the DNA for optimal interaction with the RNA polymerase.

	ToxR activates the Vibrio cholerae virulence genes by tethering DNA to the membrane through versatile binding to multiple sites.,Canals A, Pieretti S, Muriel-Masanes M, El Yaman N, Plecha SC, Thomson JJ, Fabrega-Ferrer M, Perez-Luque R, Krukonis ES, Coll M Proc Natl Acad Sci U S A. 2023 Jul 18;120(29):e2304378120. doi: , 10.1073/pnas.2304378120. Epub 2023 Jul 10. PMID:37428913<ref>PMID:37428913</ref>		==See Also==
			*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 8b4e" style="background-color:#fffaf0;"></div>~~
	== ~~References~~ ==
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:24, 9 August 2023

2023-08-09T05:24:02Z

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-The entry 8b4e is ON HOLD  until Paper Publication
+==ToxR bacterial transcriptional regulator bound to 25 bp toxT promoter DNA==
-Authors:
+ToxR activates the Vibrio cholerae virulence genes by tethering DNA to the membrane through versatile binding to multiple sites.,Canals A, Pieretti S, Muriel-Masanes M, El Yaman N, Plecha SC, Thomson JJ, Fabrega-Ferrer M, Perez-Luque R, Krukonis ES, Coll M Proc Natl Acad Sci U S A. 2023 Jul 18;120(29):e2304378120. doi: , 10.1073/pnas.2304378120. Epub 2023 Jul 10. PMID:37428913<ref>PMID:37428913</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>

OCA at 04:48, 18 January 2023

2023-01-18T04:48:58Z

← Older revision		Revision as of 04:48, 18 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8b4e is ON HOLD		The entry 8b4e is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 05:53, 21 December 2022

2022-12-21T05:53:48Z

← Older revision		Revision as of 05:53, 21 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8b4e is ON HOLD ~~until Paper Publication~~		The entry 8b4e is ON HOLD

	Authors:		Authors:

OCA at 05:07, 3 November 2022

2022-11-03T05:07:05Z

← Older revision		Revision as of 05:07, 3 November 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8b4e is ON HOLD		The entry 8b4e is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8b4e" [edit=sysop:move=sysop]

2022-09-28T04:36:45Z

Protected "8b4e" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:36, 28 September 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8b4e is ON HOLD Authors: Description: Category: Unreleased Structures

2022-09-28T04:36:44Z

New page: '''Unreleased structure''' The entry 8b4e is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8b4e is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]