8be2 - Revision history

OCA at 08:22, 14 August 2024

2024-08-14T08:22:43Z

← Older revision		Revision as of 08:22, 14 August 2024
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	<table><tr><td colspan='2'>[[8be2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BE2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8be2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BE2 FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.897935Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.897935Å</td></tr>
	~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8be2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8be2 OCA], [https://pdbe.org/8be2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8be2 RCSB], [https://www.ebi.ac.uk/pdbsum/8be2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8be2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8be2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8be2 OCA], [https://pdbe.org/8be2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8be2 RCSB], [https://www.ebi.ac.uk/pdbsum/8be2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8be2 ProSAT]</span></td></tr>
	</table>		</table>
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SOS1_HUMAN SOS1_HUMAN] Promotes the exchange of Ras-bound GDP by GTP.		[https://www.uniprot.org/uniprot/SOS1_HUMAN SOS1_HUMAN] Promotes the exchange of Ras-bound GDP by GTP.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Protein-protein interactions (PPIs) are central in cell metabolism but research tools for the structural and functional characterization of these PPIs are often missing. Here we introduce broadly applicable immunization (Cross-link PPIs and immunize llamas, ChILL) and selection strategies (Display and co-selection, DisCO) for the discovery of diverse nanobodies that either stabilize or disrupt PPIs in a single experiment. We apply ChILL and DisCO to identify competitive, connective, or fully allosteric nanobodies that inhibit or facilitate the formation of the SOS1*RAS complex and modulate the nucleotide exchange rate on this pivotal GTPase in vitro as well as RAS signalling in cellulo. One of these connective nanobodies fills a cavity that was previously identified as the binding pocket for a series of therapeutic lead compounds. The long complementarity-determining region (CDR3) that penetrates this binding pocket serves as pharmacophore for extending the repertoire of potential leads.

			Allosteric nanobodies to study the interactions between SOS1 and RAS.,Fischer B, Uchanski T, Sheryazdanova A, Gonzalez S, Volkov AN, Brosens E, Zogg T, Kalichuk V, Ballet S, Versees W, Sablina AA, Pardon E, Wohlkonig A, Steyaert J Nat Commun. 2024 Jul 23;15(1):6214. doi: 10.1038/s41467-024-50349-2. PMID:39043660<ref>PMID:39043660</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8be2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Son of sevenless 3D structures\|Son of sevenless 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:02, 1 November 2023

2023-11-01T13:02:40Z

@@ Line 1: / Line 1: @@
-The entry 8be2 is ON HOLD  until Paper Publication
+==Crystal structure of SOS1-Nanobody77==
+<StructureSection load='8be2' size='340' side='right'caption='[[8be2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8be2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BE2 FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.897935&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

OCA: Protected "8be2" [edit=sysop:move=sysop]

2022-11-03T05:09:04Z

Protected "8be2" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:09, 3 November 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8be2 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2022-11-03T05:09:03Z

New page: '''Unreleased structure''' The entry 8be2 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8be2 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]