8dds - Revision history

OCA at 05:21, 12 June 2024

2024-06-12T05:21:18Z

← Older revision		Revision as of 05:21, 12 June 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8dds]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DDS FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dds]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DDS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PH:1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE'>3PH</scene>, <scene name='pdbligand=9Z9:(3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en'>9Z9</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.5Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PH:1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE'>3PH</scene>, <scene name='pdbligand=9Z9:(3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en'>9Z9</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dds OCA], [https://pdbe.org/8dds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dds RCSB], [https://www.ebi.ac.uk/pdbsum/8dds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dds ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dds OCA], [https://pdbe.org/8dds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dds RCSB], [https://www.ebi.ac.uk/pdbsum/8dds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dds ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https~~://~~www~~.~~uniprot~~.~~org~~/~~uniprot~~/~~Q5F4S7_MOUSE Q5F4S7_MOUSE]~~		== Publication Abstract from PubMed ==
			G-protein coupled receptors (GPCRs) govern the physiological response to stimuli by modulating the activity of downstream effectors, including ion channels. TRPM3 is an ion channel inhibited by GPCRs through direct interaction with G protein (Gbetagamma) released upon their activation. This GPCR-TRPM3 signaling pathway contributes to the analgesic effect of morphine. Here, we characterized Gbetagamma inhibition of TRPM3 using electrophysiology and single particle cryo-electron microscopy (cryo-EM). From electrophysiology, we obtained a half inhibition constant (IC50) of approximately 240 nM. Using cryo-EM, we determined structures of mouse TRPM3 expressed in human cells with and without Gbetagamma and with and without PIP(2), a lipid required for TRPM3 activity, at resolutions of 2.7-4.7 A. Gbetagamma-TRPM3 interfaces vary depending on PIP(2) occupancy; however, in all cases, Gbetagamma appears loosely attached to TRPM3. The IC50 in electrophysiology experiments raises the possibility that additional unknown factors may stabilize the TRPM3-Gbetagamma complex.

			Structural and functional analyses of a GPCR-inhibited ion channel TRPM3.,Zhao C, MacKinnon R Neuron. 2023 Jan 4;111(1):81-91.e7. doi: 10.1016/j.neuron.2022.10.002. Epub 2022 , Oct 24. PMID:36283409<ref>PMID:36283409</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8dds" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:25, 3 November 2022

2022-11-03T07:25:08Z

← Older revision		Revision as of 07:25, 3 November 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8dds is ~~ON HOLD~~		==cryo-EM structure of TRPM3 ion channel in the presence of PIP2, state1==
			<StructureSection load='8dds' size='340' side='right'caption='[[8dds]], [[Resolution\|resolution]] 3.50Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[8dds]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DDS FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PH:1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE'>3PH</scene>, <scene name='pdbligand=9Z9:(3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en'>9Z9</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dds OCA], [https://pdbe.org/8dds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dds RCSB], [https://www.ebi.ac.uk/pdbsum/8dds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dds ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q5F4S7_MOUSE Q5F4S7_MOUSE]
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Mus musculus]]
			[[Category: MacKinnon R]]
			[[Category: Zhao C]]

OCA: Protected "8dds" [edit=sysop:move=sysop]

2022-06-29T06:30:02Z

Protected "8dds" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:30, 29 June 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8dds is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-29T06:30:00Z

New page: '''Unreleased structure''' The entry 8dds is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8dds is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]