8dfl - Revision history

OCA at 09:33, 17 October 2024

2024-10-17T09:33:30Z

← Older revision		Revision as of 09:33, 17 October 2024
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfl OCA], [https://pdbe.org/8dfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfl RCSB], [https://www.ebi.ac.uk/pdbsum/8dfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfl OCA], [https://pdbe.org/8dfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfl RCSB], [https://www.ebi.ac.uk/pdbsum/8dfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfl ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https://www~~.~~uniprot~~.~~org/uniprot/KCNA3_HUMAN KCNA3_HUMAN] Mediates~~ the voltage-~~dependent potassium ion permeability~~ of ~~excitable membranes. Assuming opened or closed conformations in response~~ to the ~~voltage difference across~~ the ~~membrane~~, the ~~protein forms a~~ potassium~~-selective~~ channel ~~through which potassium ions may pass in accordance~~ with ~~their electrochemical gradient~~.~~[https~~:~~//www~~.~~uniprot~~.~~org~~/~~uniprot/GFP_AEQVI GFP_AEQVI] Energy~~-~~transfer acceptor~~. ~~Its role is to transduce~~ the ~~blue chemiluminescence~~ of ~~the protein aequorin into green fluorescent light by energy transfer~~. ~~Fluoresces in vivo upon receiving energy from the Ca(2+)~~-~~activated photoprotein aequorin.~~		== Publication Abstract from PubMed ==
			The Kv1.3 potassium channel is expressed abundantly on activated T cells and mediates the cellular immune response. This role has made the channel a target for therapeutic immunomodulation to block its activity and suppress T cell activation. Here, we report structures of human Kv1.3 alone, with a nanobody inhibitor, and with an antibody-toxin fusion blocker. Rather than block the channel directly, four copies of the nanobody bind the tetramer's voltage sensing domains and the pore domain to induce an inactive pore conformation. In contrast, the antibody-toxin fusion docks its toxin domain at the extracellular mouth of the channel to insert a critical lysine into the pore. The lysine stabilizes an active conformation of the pore yet blocks ion permeation. This study visualizes Kv1.3 pore dynamics, defines two distinct mechanisms to suppress Kv1.3 channel activity with exogenous inhibitors, and provides a framework to aid development of emerging T cell immunotherapies.

			Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators.,Selvakumar P, Fernandez-Marino AI, Khanra N, He C, Paquette AJ, Wang B, Huang R, Smider VV, Rice WJ, Swartz KJ, Meyerson JR Nat Commun. 2022 Jul 4;13(1):3854. doi: 10.1038/s41467-022-31285-5. PMID:35788586<ref>PMID:35788586</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8dfl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]		*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]		*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:58, 1 May 2024

2024-05-01T07:58:22Z

← Older revision		Revision as of 07:58, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8dfl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DFL FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dfl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DFL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.25Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfl OCA], [https://pdbe.org/8dfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfl RCSB], [https://www.ebi.ac.uk/pdbsum/8dfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfl OCA], [https://pdbe.org/8dfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfl RCSB], [https://www.ebi.ac.uk/pdbsum/8dfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/KCNA3_HUMAN KCNA3_HUMAN]] Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.[[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.		[https://www.uniprot.org/uniprot/KCNA3_HUMAN KCNA3_HUMAN] Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

			==See Also==
			*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
			*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:00, 8 September 2022

2022-09-08T06:00:01Z

← Older revision		Revision as of 06:00, 8 September 2022
Line 1:		Line 1:

	====		==Structure of human Kv1.3 with A0194009G09 nanobodies (alternate conformation)==
	<StructureSection load='8dfl' size='340' side='right'caption='[[8dfl]]' scene=''>		<StructureSection load='8dfl' size='340' side='right'caption='[[8dfl]], [[Resolution\|resolution]] 3.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dfl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DFL FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfl OCA], [https://pdbe.org/8dfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfl RCSB], [https://www.ebi.ac.uk/pdbsum/8dfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfl ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfl OCA], [https://pdbe.org/8dfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfl RCSB], [https://www.ebi.ac.uk/pdbsum/8dfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfl ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/KCNA3_HUMAN KCNA3_HUMAN]] Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.[[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Aequorea victoria]]
			[[Category: Homo sapiens]]
			[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Meyerson JR]]
			[[Category: Selvakumar P]]

OCA at 05:20, 13 July 2022

2022-07-13T05:20:51Z

← Older revision		Revision as of 05:20, 13 July 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 8dfl is ~~ON HOLD~~		====
			<StructureSection load='8dfl' size='340' side='right'caption='[[8dfl]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfl OCA], [https://pdbe.org/8dfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfl RCSB], [https://www.ebi.ac.uk/pdbsum/8dfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfl ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA: Protected "8dfl" [edit=sysop:move=sysop]

2022-06-29T06:33:05Z

Protected "8dfl" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:33, 29 June 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8dfl is ON HOLD Authors: Description: Category: Unreleased Structures

2022-06-29T06:33:04Z

New page: '''Unreleased structure''' The entry 8dfl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8dfl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]