8dir - Revision history

OCA at 12:02, 23 October 2024

2024-10-23T12:02:43Z

← Older revision		Revision as of 12:02, 23 October 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dir OCA], [https://pdbe.org/8dir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dir RCSB], [https://www.ebi.ac.uk/pdbsum/8dir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dir ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dir OCA], [https://pdbe.org/8dir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dir RCSB], [https://www.ebi.ac.uk/pdbsum/8dir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dir ProSAT]</span></td></tr>
	</table>		</table>
			== Disease ==
			[https://www.uniprot.org/uniprot/IGHG1_HUMAN IGHG1_HUMAN] Defects in IGHG1 are a cause of multiple myeloma (MM) [MIM:[https://omim.org/entry/254500 254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IGHG1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~FCGR1_HUMAN FCGR1_HUMAN~~] High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.<ref>PMID:8611682</ref> <ref>PMID:9881690</ref> <ref>PMID:10397749</ref> <ref>PMID:10514529</ref>		[https://www.uniprot.org/uniprot/IGHG1_HUMAN IGHG1_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:10, 25 October 2023

2023-10-25T10:10:17Z

← Older revision		Revision as of 10:10, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8dir]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DIR FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dir]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DIR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dir OCA], [https://pdbe.org/8dir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dir RCSB], [https://www.ebi.ac.uk/pdbsum/8dir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dir ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dir OCA], [https://pdbe.org/8dir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dir RCSB], [https://www.ebi.ac.uk/pdbsum/8dir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dir ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:54, 10 May 2023

2023-05-10T06:54:53Z

← Older revision		Revision as of 06:54, 10 May 2023
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	~~'''Unreleased structure'''~~

	The ~~entry~~ 8dir is ~~ON HOLD until Paper~~ Publication		==The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R variant==
			<StructureSection load='8dir' size='340' side='right'caption='[[8dir]], [[Resolution\|resolution]] 2.30Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8dir]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DIR FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dir OCA], [https://pdbe.org/8dir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dir RCSB], [https://www.ebi.ac.uk/pdbsum/8dir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dir ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/FCGR1_HUMAN FCGR1_HUMAN] High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.<ref>PMID:8611682</ref> <ref>PMID:9881690</ref> <ref>PMID:10397749</ref> <ref>PMID:10514529</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Understanding the molecular mechanism underlying the hierarchic binding between FcgammaRs and IgG antibodies is critical for therapeutic antibody engineering and FcgammaR functions. The recent determination of crystal structures of FcgammaRI-Fc complexes, however, resulted in two controversial mechanisms for the high affinity receptor binding to IgG. Here, we describe high resolution structures of a bovine FG-loop variant of FcgammaRI in complex with the Fc fragment of IgG(1) crystallized in three different conditions at neutral pH, confirming the characteristic FG loop-Fc interaction is critical to the high affinity immunoglobulin binding. We showed that the FcgammaRI D2-domain FG-loop functioned as a pH-sensing switch for IgG binding. Further live cell imaging of FcgammaRI-mediated internalization of immune complexes showed a pH sensitive temporal-spatial antibody-antigen uptake and release. Taken together, we demonstrate that the structures of FcgammaRI-Fc crystallized at neutral and acidic pH, respectively, represent the high and low affinity binding states of the receptor for IgG uptake and release. These results support a role for FcgammaRI in antigen delivery, highlight the importance of Fc glycan in antibody binding to the high affinity receptor and provide new insights to future antibody engineering.

	~~Authors:~~ Lu, J., Sun, ~~P.D~~.		FcgammaRI FG-loop functions as a pH sensitive switch for IgG binding and release.,Lu J, Spencer M, Zou Z, Traver M, Brzostowski J, Sun PD Front Immunol. 2023 Feb 6;14:1100499. doi: 10.3389/fimmu.2023.1100499. , eCollection 2023. PMID:36814926<ref>PMID:36814926</ref>

	~~Description~~: ~~The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R variant~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased Structures~~]]		</div>
	[[Category: ~~Sun, P.D~~]]		<div class="pdbe-citations 8dir" style="background-color:#fffaf0;"></div>
	[[Category: Lu, J]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Homo sapiens]]
			[[Category: Large Structures]]
			[[Category: Lu J]]
			[[Category: Sun PD]]

OCA at 03:53, 3 August 2022

2022-08-03T03:53:37Z

← Older revision		Revision as of 03:53, 3 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8dir is ON HOLD		The entry 8dir is ON HOLD until Paper Publication

	Authors: Lu, J., Sun, P.D.		Authors: Lu, J., Sun, P.D.

OCA: Protected "8dir" [edit=sysop:move=sysop]

2022-07-06T15:15:41Z

Protected "8dir" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:15, 6 July 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8dir is ON HOLD Authors: Lu, J., Sun, P.D. Description: The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R vari...

2022-07-06T15:15:40Z

New page: '''Unreleased structure''' The entry 8dir is ON HOLD Authors: Lu, J., Sun, P.D. Description: The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R vari...

New page

'''Unreleased structure'''

The entry 8dir is ON HOLD

Authors: Lu, J., Sun, P.D.

Description: The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R variant
[[Category: Unreleased Structures]]
[[Category: Sun, P.D]]
[[Category: Lu, J]]