8dk2 - Revision history

OCA at 05:22, 12 June 2024

2024-06-12T05:22:07Z

← Older revision		Revision as of 05:22, 12 June 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8dk2]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PA14 Pseudomonas aeruginosa PA14] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DK2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DK2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dk2]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PA14 Pseudomonas aeruginosa PA14] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DK2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DK2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dk2 OCA], [https://pdbe.org/8dk2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dk2 RCSB], [https://www.ebi.ac.uk/pdbsum/8dk2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dk2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dk2 OCA], [https://pdbe.org/8dk2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dk2 RCSB], [https://www.ebi.ac.uk/pdbsum/8dk2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dk2 ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https~~://~~www~~.~~uniprot~~.~~org~~/~~uniprot~~/~~A0A0H2ZJP9_PSEAB A0A0H2ZJP9_PSEAB]~~		== Publication Abstract from PubMed ==
			Self versus non-self discrimination is a key element of innate and adaptive immunity across life. In bacteria, CRISPR-Cas and restriction-modification systems recognize non-self nucleic acids through their sequence and their methylation state, respectively. Here, we show that the Wadjet defense system recognizes DNA topology to protect its host against plasmid transformation. By combining cryoelectron microscopy with cross-linking mass spectrometry, we show that Wadjet forms a complex similar to the bacterial condensin complex MukBEF, with a novel nuclease subunit similar to a type II DNA topoisomerase. Wadjet specifically cleaves closed-circular DNA in a reaction requiring ATP hydrolysis by the structural maintenance of chromosome (SMC) ATPase subunit JetC, suggesting that the complex could use DNA loop extrusion to sense its substrate's topology, then specifically activate the nuclease subunit JetD to cleave plasmid DNA. Overall, our data reveal how bacteria have co-opted a DNA maintenance machine to specifically recognize and destroy foreign DNAs through topology sensing.

			The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.,Deep A, Gu Y, Gao YQ, Ego KM, Herzik MA Jr, Zhou H, Corbett KD Mol Cell. 2022 Nov 3;82(21):4145-4159.e7. doi: 10.1016/j.molcel.2022.09.008. Epub , 2022 Oct 6. PMID:36206765<ref>PMID:36206765</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8dk2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:36, 3 November 2022

2022-11-03T07:36:48Z

@@ Line 1: / Line 1: @@
-The entry 8dk2 is ON HOLD  until Paper Publication
+==CryoEM structure of Pseudomonas aeruginosa PA14 JetABC in an unclamped state trapped in ATP dependent dimeric form==
+<StructureSection load='8dk2' size='340' side='right'caption='[[8dk2]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8dk2]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PA14 Pseudomonas aeruginosa PA14] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DK2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DK2 FirstGlance]. <br>
-Description:
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dk2 OCA], [https://pdbe.org/8dk2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dk2 RCSB], [https://www.ebi.ac.uk/pdbsum/8dk2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dk2 ProSAT]</span></td></tr>

OCA at 16:43, 27 July 2022

2022-07-27T16:43:01Z

← Older revision		Revision as of 16:43, 27 July 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8dk2 is ON HOLD		The entry 8dk2 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8dk2" [edit=sysop:move=sysop]

2022-07-13T04:16:47Z

Protected "8dk2" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:16, 13 July 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8dk2 is ON HOLD Authors: Description: Category: Unreleased Structures

2022-07-13T04:16:46Z

New page: '''Unreleased structure''' The entry 8dk2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8dk2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]