8ea1 - Revision history

OCA at 10:19, 25 October 2023

2023-10-25T10:19:50Z

← Older revision		Revision as of 10:19, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8ea1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pueraria_montana_var._lobata Pueraria montana var. lobata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EA1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ea1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pueraria_montana_var._lobata Pueraria montana var. lobata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EA1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.29Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ea1 OCA], [https://pdbe.org/8ea1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ea1 RCSB], [https://www.ebi.ac.uk/pdbsum/8ea1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ea1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ea1 OCA], [https://pdbe.org/8ea1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ea1 RCSB], [https://www.ebi.ac.uk/pdbsum/8ea1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ea1 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:47, 30 November 2022

2022-11-30T10:47:09Z

← Older revision		Revision as of 10:47, 30 November 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8ea1 is ~~ON HOLD until Paper~~ Publication		==Structure of kudzu 2-hydroxyisoflavanone dehydratase in complex with P-NITROPHENOL==
			<StructureSection load='8ea1' size='340' side='right'caption='[[8ea1]], [[Resolution\|resolution]] 2.29Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8ea1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pueraria_montana_var._lobata Pueraria montana var. lobata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EA1 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ea1 OCA], [https://pdbe.org/8ea1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ea1 RCSB], [https://www.ebi.ac.uk/pdbsum/8ea1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ea1 ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/E9M5G1_PUEML E9M5G1_PUEML]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Isoflavonoids play important roles in plant defense and also exhibit a range of mammalian health-promoting activities. Their biosynthesis is initiated by two enzymes with unusual catalytic activities; 2-hydroxyisoflavanone synthase (2-HIS), a membrane-bound cytochrome P450 catalyzing a coupled aryl-ring migration and hydroxylation, and 2-hydroxyisoflavanone dehydratase (2-HID), a member of a large carboxylesterase family that paradoxically catalyzes dehydration of 2-hydroxyisoflavanones to isoflavone. Here we report the crystal structures of 2-HIS from Medicago truncatula and 2-HID from Pueraria lobata. The 2-HIS structure reveals a unique cytochrome P450 conformation and heme and substrate binding mode that facilitate the coupled aryl-ring migration and hydroxylation reactions. The 2-HID structure reveals the active site architecture and putative catalytic residues for the dual dehydratase and carboxylesterase activities. Mutagenesis studies revealed key residues involved in substrate binding and specificity. Understanding the structural basis of isoflavone biosynthesis will facilitate the engineering of new bioactive isoflavonoids.

	~~Authors:~~ Pan, H., ~~Wang~~, X.		The protein conformational basis of isoflavone biosynthesis.,Wang X, Pan H, Sagurthi S, Paris V, Zhuo C, Dixon RA Commun Biol. 2022 Nov 15;5(1):1249. doi: 10.1038/s42003-022-04222-x. PMID:36376429<ref>PMID:36376429</ref>

	~~Description: Structure~~ of ~~kudzu 2~~-~~hydroxyisoflavanone dehydratase in complex with P~~-~~NITROPHENOL~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: Pan, H]]		<div class="pdbe-citations 8ea1" style="background-color:#fffaf0;"></div>
	[[Category: Wang, X]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Pueraria montana var. lobata]]
			[[Category: Pan H]]
			[[Category: Wang X]]

OCA: Protected "8ea1" [edit=sysop:move=sysop]

2022-09-07T15:34:54Z

Protected "8ea1" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:34, 7 September 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8ea1 is ON HOLD until Paper Publication Authors: Pan, H., Wang, X. Description: Structure of kudzu 2-hydroxyisoflavanone dehydratase in complex wit...

2022-09-07T15:34:53Z

New page: '''Unreleased structure''' The entry 8ea1 is ON HOLD until Paper Publication Authors: Pan, H., Wang, X. Description: Structure of kudzu 2-hydroxyisoflavanone dehydratase in complex wit...

New page

'''Unreleased structure'''

The entry 8ea1 is ON HOLD until Paper Publication

Authors: Pan, H., Wang, X.

Description: Structure of kudzu 2-hydroxyisoflavanone dehydratase in complex with P-NITROPHENOL
[[Category: Unreleased Structures]]
[[Category: Pan, H]]
[[Category: Wang, X]]