8ea4 - Revision history

OCA at 06:33, 19 June 2024

2024-06-19T06:33:42Z

← Older revision		Revision as of 06:33, 19 June 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8ea4]] is a 29 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EA4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EA4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ea4]] is a 29 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EA4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EA4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ea4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ea4 OCA], [https://pdbe.org/8ea4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ea4 RCSB], [https://www.ebi.ac.uk/pdbsum/8ea4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ea4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ea4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ea4 OCA], [https://pdbe.org/8ea4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ea4 RCSB], [https://www.ebi.ac.uk/pdbsum/8ea4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ea4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A8J0PCL3_9CYAN A0A8J0PCL3_9CYAN]		[https://www.uniprot.org/uniprot/A0A8J0PCL3_9CYAN A0A8J0PCL3_9CYAN]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			CRISPR-associated transposons (CAST) are programmable mobile genetic elements that insert large DNA cargos using an RNA-guided mechanism(1-3). CAST elements contain multiple conserved proteins: a CRISPR effector (Cas12k or Cascade), a AAA+ regulator (TnsC), a transposase (TnsA-TnsB) and a target-site-associated factor (TniQ). These components are thought to cooperatively integrate DNA via formation of a multisubunit transposition integration complex (transpososome). Here we reconstituted the approximately 1 MDa type V-K CAST transpososome from Scytonema hofmannii (ShCAST) and determined its structure using single-particle cryo-electon microscopy. The architecture of this transpososome reveals modular association between the components. Cas12k forms a complex with ribosomal subunit S15 and TniQ, stabilizing formation of a full R-loop. TnsC has dedicated interaction interfaces with TniQ and TnsB. Of note, we observe TnsC-TnsB interactions at the C-terminal face of TnsC, which contribute to the stimulation of ATPase activity. Although the TnsC oligomeric assembly deviates slightly from the helical configuration found in isolation, the TnsC-bound target DNA conformation differs markedly in the transpososome. As a consequence, TnsC makes new protein-DNA interactions throughout the transpososome that are important for transposition activity. Finally, we identify two distinct transpososome populations that differ in their DNA contacts near TniQ. This suggests that associations with the CRISPR effector can be flexible. This ShCAST transpososome structure enhances our understanding of CAST transposition systems and suggests ways to improve CAST transposition for precision genome-editing applications.

			Structures of the holo CRISPR RNA-guided transposon integration complex.,Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, Kellogg EH Nature. 2023 Jan;613(7945):775-782. doi: 10.1038/s41586-022-05573-5. Epub 2022 , Nov 28. PMID:36442503<ref>PMID:36442503</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8ea4" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:47, 30 November 2022

2022-11-30T10:47:15Z

← Older revision		Revision as of 10:47, 30 November 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8ea4 ~~is ON HOLD until Paper Publication~~		==V-K CAST Transpososome from Scytonema hofmanni, minor configuration==
			<StructureSection load='8ea4' size='340' side='right'caption='[[8ea4]], [[Resolution\|resolution]] 3.00Å' scene=''>
	~~Authors~~: ~~Rizo~~, A.R., ~~Park~~, J.-U., ~~Tsai~~, A.W., ~~Kellogg, E~~.K.		== Structural highlights ==
			<table><tr><td colspan='2'>[[8ea4]] is a 29 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EA4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EA4 FirstGlance]. <br>
	~~Description~~: ~~V-K CAST Transpososome from Scytonema hofmanni, minor configuration~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ea4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ea4 OCA], [https://pdbe.org/8ea4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ea4 RCSB], [https://www.ebi.ac.uk/pdbsum/8ea4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ea4 ProSAT]</span></td></tr>
	[[Category: ~~Kellogg, E.K~~]]		</table>
	[[Category: ~~Rizo, A.R~~]]		== Function ==
	[[Category: Park, J.-U]]		[https://www.uniprot.org/uniprot/A0A8J0PCL3_9CYAN A0A8J0PCL3_9CYAN]
	[[Category: Tsai~~, A.W~~]]		__TOC__
			</StructureSection>
			[[Category: Escherichia coli]]
			[[Category: Large Structures]]
			[[Category: Scytonema hofmannii]]
			[[Category: Synthetic construct]]
			[[Category: Kellogg EK]]
			[[Category: Park J-U]]
			[[Category: Rizo AR]]
			[[Category: Tsai AW]]

OCA at 05:14, 3 November 2022

2022-11-03T05:14:44Z

← Older revision		Revision as of 05:14, 3 November 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8ea4 is ON HOLD		The entry 8ea4 is ON HOLD until Paper Publication

	Authors: Rizo, A.R., Park, J.-U., Tsai, A.W., Kellogg, E.K.		Authors: Rizo, A.R., Park, J.-U., Tsai, A.W., Kellogg, E.K.
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	Description: V-K CAST Transpososome from Scytonema hofmanni, minor configuration		Description: V-K CAST Transpososome from Scytonema hofmanni, minor configuration
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	~~[[Category: Tsai, A.W]]~~
	[[Category: Kellogg, E.K]]		[[Category: Kellogg, E.K]]
	[[Category: Rizo, A.R]]		[[Category: Rizo, A.R]]
	[[Category: Park, J.-U]]		[[Category: Park, J.-U]]
			[[Category: Tsai, A.W]]

OCA at 04:41, 28 September 2022

2022-09-28T04:41:52Z

← Older revision		Revision as of 04:41, 28 September 2022
Line 5:		Line 5:
	Authors: Rizo, A.R., Park, J.-U., Tsai, A.W., Kellogg, E.K.		Authors: Rizo, A.R., Park, J.-U., Tsai, A.W., Kellogg, E.K.

	Description: ~~13_TnsC ShCAST~~ Transpososome		Description: V-K CAST Transpososome from Scytonema hofmanni, minor configuration
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Tsai, A.W]]
	[[Category: Kellogg, E.K]]		[[Category: Kellogg, E.K]]
	[[Category: Rizo, A.R]]		[[Category: Rizo, A.R]]
	[[Category: Park, J.-U]]		[[Category: Park, J.-U]]
	~~[[Category: Tsai, A.W]]~~

OCA: Protected "8ea4" [edit=sysop:move=sysop]

2022-09-07T15:35:07Z

Protected "8ea4" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:35, 7 September 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8ea4 is ON HOLD Authors: Rizo, A.R., Park, J.-U., Tsai, A.W., Kellogg, E.K. Description: 13_TnsC ShCAST Transpososome [[Category: Unreleased Struct...

2022-09-07T15:35:06Z

New page: '''Unreleased structure''' The entry 8ea4 is ON HOLD Authors: Rizo, A.R., Park, J.-U., Tsai, A.W., Kellogg, E.K. Description: 13_TnsC ShCAST Transpososome [[Category: Unreleased Struct...

New page

'''Unreleased structure'''

The entry 8ea4 is ON HOLD

Authors: Rizo, A.R., Park, J.-U., Tsai, A.W., Kellogg, E.K.

Description: 13_TnsC ShCAST Transpososome
[[Category: Unreleased Structures]]
[[Category: Kellogg, E.K]]
[[Category: Rizo, A.R]]
[[Category: Park, J.-U]]
[[Category: Tsai, A.W]]