8fef - Revision history

OCA at 12:08, 23 October 2024

2024-10-23T12:08:47Z

← Older revision		Revision as of 12:08, 23 October 2024
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	<table><tr><td colspan='2'>[[8fef]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[8fef]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FEF FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.71Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.71Å</td></tr>
	~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>~~
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fef OCA], [https://pdbe.org/8fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fef RCSB], [https://www.ebi.ac.uk/pdbsum/8fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fef OCA], [https://pdbe.org/8fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fef RCSB], [https://www.ebi.ac.uk/pdbsum/8fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0QNR2_MYCS2 A0QNR2_MYCS2]		[https://www.uniprot.org/uniprot/A0QNR2_MYCS2 A0QNR2_MYCS2]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			To replicate inside macrophages and cause tuberculosis, Mycobacterium tuberculosis must scavenge a variety of nutrients from the host(1,2). The mammalian cell entry (MCE) proteins are important virulence factors in M. tuberculosis(1,3), where they are encoded by large gene clusters and have been implicated in the transport of fatty acids(4-7) and cholesterol(1,4,8) across the impermeable mycobacterial cell envelope. Very little is known about how cargos are transported across this barrier, and it remains unclear how the approximately ten proteins encoded by a mycobacterial mce gene cluster assemble to transport cargo across the cell envelope. Here we report the cryo-electron microscopy (cryo-EM) structure of the endogenous Mce1 lipid-import machine of Mycobacterium smegmatis-a non-pathogenic relative of M. tuberculosis. The structure reveals how the proteins of the Mce1 system assemble to form an elongated ABC transporter complex that is long enough to span the cell envelope. The Mce1 complex is dominated by a curved, needle-like domain that appears to be unrelated to previously described protein structures, and creates a protected hydrophobic pathway for lipid transport across the periplasm. Our structural data revealed the presence of a subunit of the Mce1 complex, which we identified using a combination of cryo-EM and AlphaFold2, and name LucB. Our data lead to a structural model for Mce1-mediated lipid import across the mycobacterial cell envelope.

			Structure of an endogenous mycobacterial MCE lipid transporter.,Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC Nature. 2023 Aug;620(7973):445-452. doi: 10.1038/s41586-023-06366-0. Epub 2023 , Jul 26. PMID:37495693<ref>PMID:37495693</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8fef" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:11, 2 August 2023

2023-08-02T11:11:54Z

← Older revision		Revision as of 11:11, 2 August 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8fef]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[8fef]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FEF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.71Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fef OCA], [https://pdbe.org/8fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fef RCSB], [https://www.ebi.ac.uk/pdbsum/8fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fef OCA], [https://pdbe.org/8fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fef RCSB], [https://www.ebi.ac.uk/pdbsum/8fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fef ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:45, 22 February 2023

2023-02-22T12:45:23Z

← Older revision		Revision as of 12:45, 22 February 2023
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8fef is ~~ON HOLD until Paper Publication~~		==Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)==
			<StructureSection load='8fef' size='340' side='right'caption='[[8fef]], [[Resolution\|resolution]] 2.71Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[8fef]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FEF FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fef OCA], [https://pdbe.org/8fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fef RCSB], [https://www.ebi.ac.uk/pdbsum/8fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fef ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0QNR2_MYCS2 A0QNR2_MYCS2]
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Mycolicibacterium smegmatis MC2 155]]
			[[Category: Bhabha G]]
			[[Category: Chen J]]
			[[Category: Ekiert DC]]

OCA at 05:02, 18 January 2023

2023-01-18T05:02:04Z

← Older revision		Revision as of 05:02, 18 January 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8fef is ON HOLD		The entry 8fef is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8fef" [edit=sysop:move=sysop]

2022-12-14T07:45:35Z

Protected "8fef" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:45, 14 December 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8fef is ON HOLD Authors: Description: Category: Unreleased Structures

2022-12-14T07:45:35Z

New page: '''Unreleased structure''' The entry 8fef is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8fef is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]