8ga3 - Revision history

OCA at 09:38, 17 October 2024

2024-10-17T09:38:43Z

← Older revision		Revision as of 09:38, 17 October 2024
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	==CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Turn==		==CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Turn==
	<StructureSection load='8ga3' size='340' side='right'caption='[[8ga3]]' scene=''>		<StructureSection load='8ga3' size='340' side='right'caption='[[8ga3]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GA3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ga3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GA3 FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ga3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ga3 OCA], [https://pdbe.org/8ga3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ga3 RCSB], [https://www.ebi.ac.uk/pdbsum/8ga3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ga3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ga3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ga3 OCA], [https://pdbe.org/8ga3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ga3 RCSB], [https://www.ebi.ac.uk/pdbsum/8ga3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ga3 ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			CLCs are dimeric chloride channels and anion/proton exchangers that regulate processes such as muscle contraction and endo-lysosome acidification. Common gating controls their activity; its closure simultaneously silences both protomers, and its opening allows them to independently transport ions. Mutations affecting common gating in human CLCs cause dominant genetic disorders. The structural rearrangements underlying common gating are unknown. Here, using single-particle cryo-electron microscopy, we show that the prototypical Escherichia coli CLC-ec1 undergoes large-scale rearrangements in activating conditions. The slow, pH-dependent remodeling of the dimer interface leads to the concerted opening of the intracellular H(+) pathways and is required for transport. The more frequent formation of short water wires in the open H(+) pathway enables Cl(-) pore openings. Mutations at disease-causing sites favor CLC-ec1 activation and accelerate common gate opening in the human CLC-7 exchanger. We suggest that the pH activation mechanism of CLC-ec1 is related to the common gating of CLC-7.

			Structural basis of pH-dependent activation in a CLC transporter.,Fortea E, Lee S, Chadda R, Argyros Y, Sandal P, Mahoney-Kruszka R, Ciftci HD, Falzone ME, Huysmans G, Robertson JL, Boudker O, Accardi A Nat Struct Mol Biol. 2024 Apr;31(4):644-656. doi: 10.1038/s41594-023-01210-5. , Epub 2024 Jan 26. PMID:38279055<ref>PMID:38279055</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 8ga3" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Fortea, E, Lee, S,~~ Argyos, Y, Chadda, R, Ciftci, D, Huysmans, G, Robertson, JL~~, Boudker, O, Accardi, A~~]]		[[Category: Accardi A]]
			[[Category: Argyos Y]]
			[[Category: Boudker O]]
			[[Category: Chadda R]]
			[[Category: Ciftci D]]
			[[Category: Fortea E]]
			[[Category: Huysmans G]]
			[[Category: Lee S]]
			[[Category: Robertson JL]]

OCA at 06:19, 7 February 2024

2024-02-07T06:19:49Z

← Older revision		Revision as of 06:19, 7 February 2024
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	~~'''Unreleased structure'''~~

	~~The entry 8ga3 is ON HOLD until Paper Publication~~		==CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Turn==
			<StructureSection load='8ga3' size='340' side='right'caption='[[8ga3]]' scene=''>
	~~Authors: Fortea, E., Lee, S., Argyos, Y., Chadda, R., Ciftci, D., Huysmans, G., Robertson, J.L., Boudker, O., Accardi, A.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GA3 FirstGlance]. <br>
	~~Description:~~ CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Turn		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy</td></tr>
	[[~~Category: Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ga3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ga3 OCA], [https://pdbe.org/8ga3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ga3 RCSB], [https://www.ebi.ac.uk/pdbsum/8ga3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ga3 ProSAT]</span></td></tr>
	[[~~Category~~: ~~Robertson, J~~.L]]		</table>
	[[~~Category~~: ~~Boudker, O~~]]		__TOC__
	[[~~Category~~: ~~Ciftci~~, D]]		</StructureSection>
	[~~[Category~~: ~~Fortea~~, ~~E]]~~		[[Category: Large Structures]]
	[[~~Category~~: ~~Lee, S]~~]		[[Category: Fortea, E, Lee, S, Argyos, Y, Chadda, R, Ciftci, D, Huysmans, G, Robertson, JL, Boudker, O, Accardi, A]]
	[[Category: ~~Accardi, A~~]]
	[[Category: Argyos, Y]]
	~~[[Category:~~ Huysmans, G]]
	~~[[Category: Chadda~~, R]]

OCA at 04:45, 29 March 2023

2023-03-29T04:45:35Z

← Older revision		Revision as of 04:45, 29 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8ga3 is ON HOLD		The entry 8ga3 is ON HOLD until Paper Publication

	Authors: Fortea, E., Lee, S., Argyos, Y., Chadda, R., Ciftci, D., Huysmans, G., Robertson, J.L., Boudker, O., Accardi, A.		Authors: Fortea, E., Lee, S., Argyos, Y., Chadda, R., Ciftci, D., Huysmans, G., Robertson, J.L., Boudker, O., Accardi, A.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Robertson, J.L]]		[[Category: Robertson, J.L]]
	~~[[Category: Accardi, A]]~~
	[[Category: Boudker, O]]		[[Category: Boudker, O]]
	~~[[Category: Lee, S]]~~
	~~[[Category: Chadda, R]]~~
	[[Category: Ciftci, D]]		[[Category: Ciftci, D]]
	[[Category: Fortea, E]]		[[Category: Fortea, E]]
			[[Category: Lee, S]]
			[[Category: Accardi, A]]
	[[Category: Argyos, Y]]		[[Category: Argyos, Y]]
	[[Category: Huysmans, G]]		[[Category: Huysmans, G]]
			[[Category: Chadda, R]]

OCA: Protected "8ga3" [edit=sysop:move=sysop]

2023-03-02T04:33:17Z

Protected "8ga3" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:33, 2 March 2023
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8ga3 is ON HOLD Authors: Fortea, E., Lee, S., Argyos, Y., Chadda, R., Ciftci, D., Huysmans, G., Robertson, J.L., Boudker, O., Accardi, A. Descripti...

2023-03-02T04:33:16Z

New page: '''Unreleased structure''' The entry 8ga3 is ON HOLD Authors: Fortea, E., Lee, S., Argyos, Y., Chadda, R., Ciftci, D., Huysmans, G., Robertson, J.L., Boudker, O., Accardi, A. Descripti...

New page

'''Unreleased structure'''

The entry 8ga3 is ON HOLD

Authors: Fortea, E., Lee, S., Argyos, Y., Chadda, R., Ciftci, D., Huysmans, G., Robertson, J.L., Boudker, O., Accardi, A.

Description: CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Turn
[[Category: Unreleased Structures]]
[[Category: Robertson, J.L]]
[[Category: Accardi, A]]
[[Category: Boudker, O]]
[[Category: Lee, S]]
[[Category: Chadda, R]]
[[Category: Ciftci, D]]
[[Category: Fortea, E]]
[[Category: Argyos, Y]]
[[Category: Huysmans, G]]